Studies of gene function in non-model animals have been limited by the approaches available for eliminating function. The CRISPR/Cas9 ( C: lustered R: egularly I: nterspaced S: hort P: alindromic epeats/ RISPR AS: sociated) system has recently become a powerful tool targeted genome editing. Here, we report use to disrupt selected genes, including nanos2, nanos3, dmrt1, and foxl2, with efficiencies as high 95%. In addition, mutations dmrt1 foxl2 induced were efficiently transmitted through...

It is well accepted that Forkhead box protein L2 (Foxl2) and aromatase (Cyp19a1; the enzyme responsible for estrogen synthesis) are critical ovarian development in vertebrates. Knockouts of Foxl2 Cyp19a1 goat, mouse, zebrafish have revealed similar but not identical functions across species. Functional analyses these two genes other animals needed to elucidate their conserved roles vertebrate sexual development. In this study, we established foxl2 cyp19a1a mutant lines Nile tilapia. Both...

SUMMARY Gonadal soma‐derived factor ( gsdf ) is critical for testicular differentiation in teleosts, yet detailed analysis of Gsdf on lacking. In the present study, we knocked out tilapia using CRISPR/Cas9. F0 gsdf‐ deficient XY fish with high mutation rate (≥58%) developed as intersex, ovotestes 90 days after hatching (dah), and become completely sex‐reversed ovaries at 180 240 dah. Those individuals a low (<58%) +/− males normal testes. F2 −/− fish, gonads first expressed Dmrt1, which...

Multidrug resistance (MDR) has become a major impediment to successful treatment for liver cancer patients, and one of the common reasons MDR is activation ABCB1 gene, leading over-expression P-glycoprotein (P-gp), which conferred cells be resistant broad range anticancer drugs. MicroRNAs (miRNAs) are class short, non-coding RNA moleculars that can regulate gene expression at post-transcriptional level. In current study, aim explore whether miRNA participates in regulation mediated by ABCB1....

Continuous plasma separation will be greatly helpful for dynamic metabolite monitoring in kinetics research and drug development. In this work, we proposed a continuous on-chip method based on the natural aggregating sedimentation behavior of red blood cells at low shear rate. approach, glass capillary was first used to realize quick obvious delamination from plasma. A novel "dual-elbow" connector designed change direction delamination. The finally separated by laminar flow bifurcation...

Water deficit and soil salinity lead to lower yield by limiting fruit growth. Investigating the process mechanisms which water salt stress limit growth is important fully exploit biological agronomic saving potential of tomato in saline soils. We set up two irrigation regimes (W0, 90% field capacity as upper limit; W3, 60% limit) (S0, no added; S3, was added at 3 g Kg−1 dry soil) investigate effects on carbon input rate, volume expansion difference between plant stem (water gradient),...

The dmrt6 gene has been isolated from tetrapods and recently a coelacanth, Latimeria chalumnae. Its evolutionary history exact function remain unclear. In the present study, was Perciformes (five cichlids stickleback), Siluriformes (southern catfish), Lepisosteiformes (spotted gar). Syntenic phylogenetic analyses indicated that experienced transposition after divergence of teleosts other bony fish as loci surrounding were conserved among (but completely different in spotted gar), while these...

The experiment was aimed at examining the influence of adding emodin to feeds on growth performance, liver immunity, and resistance against Aeromonas veronii infection among juvenile largemouth basses other potential mechanisms. A total 540 fish (45 ± 0.3 g) were randomly divided into 6 diets, including EM-0, EM-250, EM-500, EM-1000, EM-2000, EM-4000 in which 0, 250, 500, 1000, 2000, 4000 mg kg-1 added. Following a 60-day feeding test, it demonstrated that feed conversion ratio (FCR)...

The mitotic kinesin superfamily protein KIF14 is essential for cytokinesis and chromosome segregation increased expression related to a variety of human cancers. In this study, we investigate in association with clinical variables the role during tumorigenesis. We found that overexpressed most primary hepatocellular carcinoma (HCC) tissues compared adjacent normal liver overexpression associated tumor grade (P = 0.002), stage 0.013) poor survival < 0.001). Downregulation decreased capacity...

Endogenous viral elements play important roles in eukaryotic evolution by giving rise to genetic novelties. Herpesviruses are a large family of DNA viruses, most which do not have the ability endogenize into host genomes. Recently, we identified novel type endogenous herpesvirus, named "Teratorn", from medaka (Oryzias latipes) genome, herpesvirus is fused with piggyBac-like transposon, forming mobile element. Teratorn unique that retains its genes intact and has acquired endogenized...

Type 2 diabetes mellitus (T2DM) is a long-term metabolic disorder. It characterized by hyperglycemia, insulin resistance (IR), and relative impairment in secretion. IR plays major role the pathogenesis of T2DM. Many previous studies have investigated relationship between estrogen, androgen, obesity, but few focused on sex hormones, abnormal lipid metabolism, IR. The goal for present study was to identify association with hormone, metabolism type diabetes, impaired glucose tolerance (IGT)...

Abstract Background This study aimed to assess the different impacts of aerobic and resistance exercise intervention on pre-diabetes its possible influencing factor (obesity) identify which mode was better for control their blood glucose levels. Methods Single-blind randomized controlled trial. Participants were recruited from Southwest Hospital between February 2016 May 2017 randomly divided into three groups using stratified randomization: (A), (R), (C). The effects each group analyzed,...

PurposeThe Adhesion G protein receptor E5 (ADGRE5) gene is involved in a wide range of biological functions human tumors; however, its specific molecular mechanism and significance the analysis tumors have not yet been determined. Here, we provide comprehensive genomic architecture ADGRE5 tumor immune microenvironment clinical relevance across broad solid tumors.MethodsIn this study, used publicly available bioinformatics databases, with primary focus on The Cancer Genome Atlas (TCGA)...

We report an approach for fabricating a tunable wettability surface by electroless gold plating on poly(dimethylsiloxane) (PDMS). A two-layer structured film with tight layer and loose can be obtained the of PDMS chip when is immersed in solution at 30 °C 4 h. Its rapidly switched between superhydrophilicity superhydrophobicity plasma heat treatments without any self-assembled monolayer, even changed from gecko-foot-hair-like character to lotus-leaf-like character. Benefiting various...

Abstract Xylem water transport from the parent plant plays a crucial role in fruit growth, development, and determination of quality. Attempts have been made to partition hydraulic resistance pathway over course but no consensus has reached. Furthermore, issue not addressed context changing status under deficit conditions. In this study, we conducted rigorous investigation into developmental changes that occur properties tomato fruits their pedicels well-irrigated conditions, based on...

Prediction of crop yield under water and salt stress is essential for guiding deficit irrigation in saline soil. The responses tomato plants to stress, their interactions have profound effect on yield. Traditional crop-water-salt production functions do not fully account the physiological response all stresses, especially interaction soil salinity deficit. We developed a static function based traditional function, combined with between specific effects salinity, statistical model. This...

The involvement of estrogen in male fertility has been well established mammals. However, less is known about the role fish reproduction. Our recent study revealed that Cyp19a1a deficiency had no effect on fish. In this study, expression Cyp19a1b, but not Cyp19a1a, was detected by immunohistochemistry Leydig cells tilapia testes. cyp19a1b mutation resulted a significant decrease concentration 17β-estradiol serum and sterility XY fish, as offspring were obtained when crossed with control XX...

Abstract Aims/Introduction To estimate the prevalence, and patient clinical demographic profile, as well risk factors associated with obstructive sleep apnea syndrome (OSAS) in hospitalized patients type 2 diabetes mellitus Beijing, China. Materials Methods Hospitalized adult were consecutively screened invited for an overnight polysomnography from four hospitals China, May 2016 to February 2017. We used American Academy of Sleep Medicine 2012 recording techniques scoring criteria identify...

Abstract Background MicroRNAs (miRNAs) are endogenous small non-coding RNAs (21–25 nucleotides) that act as essential components of several biological processes. Golden-back crucian carp (GBCrC, Carassius auratus ) is a naturally mutant species has two distinct body skin color types (golden and greenish-grey), making it an excellent model for research on the genetic basis pigmentation. Here, we performed RNA (sRNA) analysis different colors via Illumina sequencing. Results A total 679 known...

Cancer cells exhibit elevated levels of glucose uptake and may obtain pre-formed, diet-derived fatty acids from the bloodstream to boost their rapid growth; they also use nucleic acid microenvironment. The study processing by cancer will help improve understanding metabolism cancer. DNA is commonly packaged into a viral or lipid particle be transferred cells; this process called transfection in laboratory. are known for having gene mutations evolving ability endocytosis. Their DNAs might...