A5046393652- Chromosomal and Genetic Variations
- Genomics and Phylogenetic Studies
- CRISPR and Genetic Engineering
- Genetic diversity and population structure
- RNA and protein synthesis mechanisms
- Plant Virus Research Studies
- Animal Genetics and Reproduction
- melanin and skin pigmentation
- Aquaculture disease management and microbiota
- Biochemical Analysis and Sensing Techniques
- Advanced biosensing and bioanalysis techniques
- Genomics and Chromatin Dynamics
- Genomic variations and chromosomal abnormalities
- Primate Behavior and Ecology
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- RNA Interference and Gene Delivery
- Identification and Quantification in Food
- Animal Behavior and Reproduction
- Evolution and Genetic Dynamics
- RNA modifications and cancer
- Congenital heart defects research
- Livestock and Poultry Management
- RNA regulation and disease
- Telemedicine and Telehealth Implementation
- Retinal Development and Disorders
Kyoto University
2015-2024
Kasetsart University
2013-2024
National Institute of Diabetes and Digestive and Kidney Diseases
2023
ProQuest (United States)
2023
Institute of Primate Research
2009-2021
Nagoya University
2001-2013
Zoological Park Organization
2013
Hokkaido University
2013
Daiichi University of Pharmacy
2003
Frontier Science Foundation
2003
Abstract A chimeric white gene (wpch) and other constructs containing the transposable element mariner from Drosophila mauritiana were introduced into germline of melanogaster using transformation mediated by P element. In absence elements, wpch allele is genetically stable in both germ cells somatic cells, indicating that peach (i.e., particular copy inserted allele) inactive. However, presence active Mos1, reverts, owing to excision element, yielding eye-color mosaics a high rate...
Mobile genetic elements (e.g., transposable and viruses) display significant diversity with various life cycles, but how novel emerge remains obscure. Here, we report a giant (180-kb long) transposon, Teratorn, originally identified in the genome of medaka, Oryzias latipes. Teratorn belongs to piggyBac superfamily retains transposition activity. Remarkably, is largely derived from herpesvirus Alloherpesviridae family that could infect fish amphibians. Genomic survey Teratorn-like reveals...
Hybrid sterility, a hallmark of postzygotic isolation, arises from parental genome divergence disrupting meiosis. While chromosomal incompatibility is often implicated, the underlying mechanisms remain unclear. This study investigated meiotic behavior and genome-wide in bighead catfish (C. macrocephalus), North African gariepinus), their sterile male hybrids (important aquaculture). Repetitive DNA analysis using bioinformatics cytogenetics revealed significant satellite (satDNA) families...
Microsatellites are polymorphic and cost-effective. Optimizing reduced microsatellite panels using heuristic algorithms eases budget constraints in genetic diversity population assessments. Microsatellite marker efficiency is strongly associated with its polymorphism quantified as the information content (PIC). Nevertheless, selection cannot rely solely on PIC. In this study, ant colony optimization (ACO) algorithm, a widely recognized method, was adopted to create an enhanced scheme for...
The thermal stress caused by global climate change adversely affects the welfare, productivity, and reproductive performance of farm animals, including chickens, causes substantial economic losses. However, understanding genetic basis indigenous chicken adaptation to high ambient temperatures is limited. Hence, reveal in this study investigated polymorphisms heat shock protein 70 (HSP70) HSP90 genes, known mechanisms cellular defense against local breeds red junglefowls Thailand. result...
The North African catfish ( Clarias gariepinus ) is a significant species in aquaculture, which crucial for ensuring food and nutrition security. Their high adaptability to diverse environments has led an increase the number of farms that are available their production. However, long-term closed breeding adversely affects reproductive performance, leading decrease production efficiency. This possibly caused by inbreeding depression. To investigate root cause this issue, genetic diversity...
Abstract Tol1 is a DNA‐based transposable element first identified from an albino mutant medaka fish. It has been demonstrated to function as efficient gene transfer vector in mammalian cells. We now demonstrate germline transgenesis zebrafish. A construct containing the green fluorescence protein (GFP) reporter inserted between arms was microinjected together with transposase mRNA into fertilized eggs. Sustained GFP expression observed 88% of 1‐month‐old fish, suggesting transposon...
Endogenous viral elements play important roles in eukaryotic evolution by giving rise to genetic novelties. Herpesviruses are a large family of DNA viruses, most which do not have the ability endogenize into host genomes. Recently, we identified novel type endogenous herpesvirus, named "Teratorn", from medaka (Oryzias latipes) genome, herpesvirus is fused with piggyBac-like transposon, forming mobile element. Teratorn unique that retains its genes intact and has acquired endogenized...
Abstract Tol2 is a transposable element of the terminal-inverted-repeat class, residing in genome medaka fish Oryzias latipes. The genus contains more than 10 species for which phylogenetic relationships have previously been estimated. To infer history this we performed genomic Southern blots and PCR analyses species. It was revealed that occurs 2 (O. curvinotus O. latipes) length restriction map structure are identical two cases. Further, sequencing analysis an extremely low level...
Abstract Active and inactive mariner elements from natural laboratory populations of Drosophila simulans were isolated sequenced in order to assess their nucleotide variability compare them with previously the sibling species mauritiana sechellia. The active D. are very similar among themselves (average 99.7% identity), suggesting that level expression different is largely determined by position effects, dosage effects perhaps other factors. Furthermore, exhibit identities 98% or greater...
DNA-based transposable elements, or DNA transposons, transpose in a cut-and-paste fashion, involving excision from the chromosome. If this process affects function of host gene and rate is high, any associated with such an element would clearly be genetically "unstable" state, there are many examples unstable genes various organisms. However, none have hitherto been reported vertebrates. We here document finding mutant medaka fish, Oryzias latipes, useful model animal for vertebrate genetics...
Alpha-satellite DNA (AS) is a main component of primate centromeres, consisting tandemly repeated units ∼170 bp. The AS humans contains sequences organized into higher-order repeat (HOR) structures, in which block multiple forms larger unit and the are tandemly. presence HOR widely thought to be unique hominids (family Hominidae; great apes). Recently, we have identified an HOR-containing siamang, small ape species belonging genus Symphalangus family Hylobatidae. This result supports view...
Abstract Centromeres usually contain large amounts of tandem repeat DNA. Alpha satellite DNA (AS) is the most abundant found in centromeres simian primates. The AS humans contains sequences organized into higher-order (HOR) structures, which are arrays larger units consisting multiple basic units. HOR-carrying also occurs other hominoids, but results reported to date for phylogenetically more remote taxa have been negative. Here we show direct evidence clear HOR structures owl monkey and...
Centromere protein B (CENP-B) is one of the major proteins involved in centromere formation, binding to centromeric repetitive DNA by recognizing a 17 bp motif called CENP-B box. Hominids (humans and great apes) carry large numbers boxes alpha satellite (AS, simian primates). Only negative results have been reported regarding presence box other primate taxa. Consequently, it widely believed that confined, within primates, hominids. We report here common marmoset, New World monkey, contains...
In recent decades, a global acceleration of urbanization has been observed, leading to significant habitat loss and fragmentation. Economic development in eastern Thailand promoted, but this often coincided with unsustainable exploitation natural resources. The expansion urban industrial areas the region puts many organisms at risk extinction owing loss. Plakad-Pa Pak-Tawan-Ok (Betta siamorientalis) is an endemic species found only Thailand. study, genetic diversity population structure 17...
Polycystic kidney disease (PKD) is a common hereditary in humans. Recent studies have shown an increasing number of ciliary genes that are involved the pathogenesis PKD. In this study, Gli-similar3 (glis3) gene was identified as causal medaka pc mutant, model transposon found to be inserted into fourth intron pc/glis3 gene, causing aberrant splicing mRNA and thus putatively truncated protein with defective zinc finger domain. expressed epithelial cells renal tubules ducts pronephros...
Alpha satellite DNA is a repetitive sequence known to be major component of centromeres in primates (order Primates). New World monkeys form one taxon (parvorder Platyrrhini) primates, and their alpha comprise repeat units around 340 bp. In species (Azara's owl monkey Aotus azarae) this taxon, we identified two types consisting 185- 344-bp that designated as OwlAlp1 OwlAlp2, respectively. OwlAlp2 exhibits similarity throughout its entire the other monkeys. The chromosomal locations are...
Two novel repetitive DNA sequences, VSAREP1 and VSAREP2, were isolated from the water monitor lizard (Varanus salvator macromaculatus, Platynota) characterized using molecular cytogenetics. The respective lengths guanine-cytosine (GC) contents of sequences 190bp 57.5% for 185bp 59.7% both elements tandemly arrayed as satellite in genome. VSAREP2 each located at C-positive heterochromatin pericentromeric region chromosome 2q, centromeric 5, 3 pairs microchromosomes. This suggests that genomic...