A5035319682- Chronic Myeloid Leukemia Treatments
- Zebrafish Biomedical Research Applications
- Chronic Lymphocytic Leukemia Research
- Neurogenesis and neuroplasticity mechanisms
- Eosinophilic Disorders and Syndromes
- Acute Myeloid Leukemia Research
- Cancer-related gene regulation
- Epigenetics and DNA Methylation
- Fibroblast Growth Factor Research
- Myeloproliferative Neoplasms: Diagnosis and Treatment
- Bone health and treatments
- RNA Interference and Gene Delivery
- Retinal Development and Disorders
- Bone Metabolism and Diseases
- Advanced biosensing and bioanalysis techniques
- Forest Insect Ecology and Management
- MicroRNA in disease regulation
- Insect and Arachnid Ecology and Behavior
- Lymphoma Diagnosis and Treatment
- Wnt/β-catenin signaling in development and cancer
- Pluripotent Stem Cells Research
- Amyloidosis: Diagnosis, Treatment, Outcomes
- Hemiptera Insect Studies
- Developmental Biology and Gene Regulation
- CRISPR and Genetic Engineering
University of Tsukuba
2005-2023
Nagoya University
2014-2020
Hoshi University
2016
Ebara (Japan)
2016
Kyoto University Hospital
2007-2011
Kyoto University
2007-2011
Shiga University of Medical Science
2007-2010
Kanagawa Cancer Center
2008
Kanagawa Prefectural Hospital Organization
2008
Aichi Cancer Center
1991
Abstract LSD1/KDM1A is a widely conserved lysine-specific demethylase that removes methyl groups from methylated proteins, mainly histone H3. We previously isolated the zebrafish LSD1 gene and demonstrated it required for primitive hematopoiesis. Recently, neuron-specific splicing variant of was found in mammals its specific functions substrate specificities were reported. To our surprise, cDNA, which we analyzed, corresponded to mammals. In this study, investigated structures expression...
Conventionally, comparison among amniotes - birds, mammals, and reptiles has often been approached through analyses of mammals and, for comparison, birds. However, birds are morphologically physiologically derived moreover, some parts their genomes recognized as difficult to sequence and/or assemble thus missing in genome assemblies. Therefore, sequencing the would aid comparative studies on by providing more comprehensive coverage help understand molecular mechanisms underpinning...
Pi class GSTs (glutathione S-transferases) are a member of the vertebrate GST family proteins that catalyse conjugation GSH to electrophilic compounds. The expression genes can be induced by exposure electrophiles. We demonstrated previously transcription factor Nrf 2 (NF-E2 p45-related 2) mediates this induction, not only in mammals, but also fish. In present study, we have isolated genomic region zebrafish containing gstp1 and gstp2. regulatory regions gstp2 been examined GFP (green...
Summary The effect of ABT‐737, a BH3‐mimicking inhibitor for anti‐apoptotic Bcl‐2 and Bcl‐X L , but not Mcl‐1, against Bcr‐Abl‐positive (Bcr‐Abl + ) leukaemic cells was examined. ABT‐737 potently induced apoptosis in Bcr‐Abl chronic myeloid leukaemia (CML) cell lines primary CML samples vitro prolonged the survival mice xenografted with BV173 cells, line. Higher expression proteins reduced killing by each line, there no correlation between sensitivities to specific patterns family among...
PRMT8 directly hydrolyzes phosphatidylcholine, which is important for brain functions.
beta-catenin is the downstream effector of Wnt signaling pathway, and it regulates cell proliferation. overexpression correlates positively with prognosis in several types malignancies. We herein assessed its effects on growth multiple myeloma cells using a xenograft model.We first investigated expression lines obtained from patients. Next, we inhibitory small interfering RNA vivo. Six-week-old male BALB/c nu/nu mice were inoculated s.c. right flank 5 x 10(6) RPMI8226 cells, followed by...
Abstract Liver metastasis is one of the most important prognostic factors in lung cancer patients. However, current therapies are not sufficient. RNA interference provides us a powerful and promising approach for treating human diseases including cancers. Herein, we investigated vitro effects PLK-1 small interfering (siRNA) on cell lines vivo usage siRNA with atelocollagen as drug delivery system murine liver model cancer. was overexpressed cancerous tissues from treatment inhibited growth...
Galectins comprise a family of animal lectins that differ in their affinity for β-galactosides. Galectin-9 (Gal-9) is tandem-repeat-type galectin was recently shown to function as ligand T-cell immunoglobin domain and mucin domain-3 (Tim-3) expressed on terminally differentiated CD4(+) Th1 cells. Gal-9 modulates immune reactions, including the induction apoptosis In this study, we investigated effects murine models acute GVH disease (aGVHD). First, demonstrated recombinant human inhibit MLR...
Multiple myeloma (MM) is a malignant neoplasm of plasma cells. Although new molecular targeting agents against MM have been developed based on the better understanding underlying pathogenesis, still remains an incurable disease. We previously demonstrated that β-catenin, downstream effector in Wnt pathway, potential target using RNA interference vivo experimental mouse model. In this study, we screened library more than 100 000 small-molecule chemical compounds for novel Wnt/β-catenin...
Significance Epigenetic regulation of cell fate determination is one the hottest topics today. In this study, we isolated and characterized a zebrafish mutant in histone Lys-specific demethylase 1 (LSD1/KDM1A), found that LSD1 plays role initiation hematopoietic differentiation hemangioblast, bipotent can give rise to or endothelial progenitors. addition, identified major function hemangioblasts be down-regulation Ets variant 2 (Etv2), critical regulator hemangioblast development. Our...
Abstract The structure of the neural circuitry cerebellum, which functions in some types motor learning and coordination, is generally conserved among vertebrates. However, cerebellar features are species specific. It not clear genes involved forming these species‐specific structures functions. This study uses zebrafish transgenic larvae expressing fluorescent proteins granule cells, Purkinje or other neurons glial cells to isolate each type by fluorescence‐activated cell sorting profile...
Granule cells (GCs) are the major glutamatergic neurons in cerebellum, and GC axon formation is an initial step establishing functional cerebellar circuits. In zebrafish GCs can be classified into rostromedial caudolateral groups, according to locations of their somata corresponding lobes. The axons lobes terminate on crest dorsal hindbrain, as well forming en passant synapses with Purkinje cerebellum. mutant shiomaneki, extend aberrant axons. Positional cloning revealed that shiomaneki...
Gastric inhibitory polypeptide plays a role in glucose and lipid metabolism is associated with obesity insulin resistance. The objective of this study to confirm the anti-obesity effects gastric receptor antagonist, SKL-14959, on diet-induced mice.Diet-induced mice at 20 weeks age were administered or without SKL-14959 for 96 d. Body weight food intake monitored throughout experiment. Mice sacrificed, physiological biochemical markers measured, then histochemical gene expression analyses...
Treatment with Abl tyrosine kinase inhibitors (TKI) drastically improves the prognosis of chronic myelogenous leukemia (CML) patients. However, quiescent CML cells are insensitive to TKI and can lead relapse disease. Thus, research is needed elucidate properties these cells, including their microenvironment, in order effectively target them. Hypoxia known be a common feature solid tumors that contributes therapeutic resistance. Leukemic also able survive proliferate severely hypoxic...