A5031030047- Plant Molecular Biology Research
- Photosynthetic Processes and Mechanisms
- Light effects on plants
- Plant Reproductive Biology
- Plant tissue culture and regeneration
- Plant nutrient uptake and metabolism
- Advanced Fluorescence Microscopy Techniques
- Luminescence and Fluorescent Materials
- Crystallization and Solubility Studies
- X-ray Diffraction in Crystallography
- Microtubule and mitosis dynamics
- Molecular Sensors and Ion Detection
- Mitochondrial Function and Pathology
- Plant and Biological Electrophysiology Studies
- Click Chemistry and Applications
- Fern and Epiphyte Biology
- Genetics, Aging, and Longevity in Model Organisms
- Radical Photochemical Reactions
- RNA Interference and Gene Delivery
- Plant Genetic and Mutation Studies
- Genomics and Chromatin Dynamics
- Nanoplatforms for cancer theranostics
- Plant Parasitism and Resistance
- Plant Diversity and Evolution
- Slime Mold and Myxomycetes Research
Nagoya University
2016-2025
National Institute for Basic Biology
2003-2023
Japan Science and Technology Agency
2003-2017
Konan University
2013
Osaka University
2011
Denka (Japan)
2009
Tokyo University of Agriculture and Technology
2004
Tokyo Metropolitan University
1999-2003
Okazaki Institute for Integrative Bioscience
2003
Kobe Shoin Women's University
1981
Vascular plants appeared ~410 million years ago, then diverged into several lineages of which only two survive: the euphyllophytes (ferns and seed plants) lycophytes. We report here genome sequence lycophyte Selaginella moellendorffii (Selaginella), first nonseed vascular plant reported. By comparing gene content in evolutionarily diverse taxa, we found that transition from a gametophyte- to sporophyte-dominated life cycle required far fewer new genes than flowering plant, whereas secondary...
Imaging techniques for visualizing and analyzing precise morphology gene expression patterns are essential understanding biological processes during development in all organisms. With the aid of chemical screening, we developed a clearing method using solutions, termed ClearSee, deep imaging plant tissues. ClearSee rapidly diminishes chlorophyll autofluorescence while maintaining fluorescent protein stability. By adjusting refractive index mismatch, whole-organ whole-plant can be performed...
Germination signals illuminated The noxious weed Striga can take down an entire crop. Fields in Africa are particularly susceptible to the devastation it cause. seeds germinate response faint traces of hormone strigolactone released by its targets. Tsuchiya et al. designed a mimic that, when cleaved receptor, generates fluorescent end product. This photogenic lit up upon germination and led identification receptor. Abolishing activity this receptor could be effective defensive strategy....
Significance Stimulated emission depletion (STED) microscopy is one of the most appealing tools to visualize nanoscale cellular structures and dynamics in living cells. However, its practical utility significantly limited by rapid photobleaching fluorescent dyes under ultrastrong laser. In this context, superphotostable probes, which enable repeated recording STED images, are crucial chemical extend application live-cell imaging. Herein, we report a developed probe, MitoPB Yellow, has...
Abstract Electron‐donating aryl groups were attached to electron‐accepting benzophosphole skeletons. Among several derivatives thus prepared, one oxide was particularly interesting, as it retained high fluorescence quantum yields even in polar and protic solvents. This phosphole‐based compound exhibited a drastic color change of its spectrum function the solvent polarity, while absorption spectra remained virtually unchanged. Capitalizing on these features, this used stain adipocytes, which...
Plant grafting is conducted for fruit and vegetable propagation, whereby a piece of living tissue attached to another through cell-cell adhesion. However, graft compatibility limits combinations closely related species, the mechanism poorly understood. We found that Nicotiana capable adhesion with diverse range angiosperms. Comparative transcriptomic analyses on indicated subclade β-1,4-glucanases secreted into extracellular region facilitates cell wall reconstruction near interface....
Significance In animals and plants, the zygote divides unequally, daughter cells inherit different developmental fates to form a proper embryo along body axis. The cytological events leading polarization have remained unknown in flowering plants. Here, we report that two essential components of cytoskeleton, microtubules actin filaments, are both disorganized on fertilization then, arranged transverse ring directional cell elongation longitudinal arrays underlying polar nuclear migration,...
As stimulated emission depletion (STED) microscopy can provide structural details of cells with an optical resolution beyond the diffraction limit, it has become indispensable tool in cell biology. However, intense STED laser beam usually causes rapid photobleaching employed fluorescent dyes, which significantly limits utility from a practical perspective. Herein we report new design super-photostable dye, PhoxBright 430 (PB430), comprising fully ring-fused π-conjugated skeleton...
Many differentiated plant cells can dedifferentiate into stem cells, reflecting the remarkable developmental plasticity of plants. In moss Physcomitrella patens, at wound margin detached leaves become reprogrammed cells. Here, we report that two paralogous P. patens WUSCHEL-related homeobox 13-like (PpWOX13L) genes, homologs cell regulators in flowering plants, are transiently upregulated and required for initiation growth during formation. Concordantly, Δppwox13l deletion mutants fail to...
Various fluorescence microscopy techniques require bright NIR-emitting fluorophores with high chemical and photostability. Now, the significant performance improvement of phosphorus-substituted rhodamine dyes (PORs) upon substitution at 9-position a 2,6-dimethoxyphenyl group is reported. The thus obtained dye PREX 710 was used to stain mitochondria in living cells, which allowed long-term three-color imaging vis-NIR range. Moreover, longevity allows tracking dye-labeled biomolecule by...
Abstract The development of stimulated emission depletion (STED) microscopy represented a major breakthrough in cellular and molecular biology. However, the intense laser beams required for both excitation STED usually provoke rapid photobleaching fluorescent probes, which significantly limits performance practical utility microscopy. We herein developed photoresistant dye C‐Naphox as tool imaging. With using either λ =405 or 488 nm protic solvents, exhibited an red/orange fluorescence...
Neurite growth requires two guanine nucleotide-binding protein polymers of tubulins and septins. However, whether how those cytoskeletal systems are coordinated was unknown. Here we show that the acute knockdown or knockout pivotal septin subunit SEPT7 from cerebrocortical neurons impairs their interhemispheric cerebrospinal axon projections dendritogenesis in perinatal mice, when microtubules severely hyperacetylated. The resulting hyperstabilization retardation demonstrated vitro....
Abstract Nanographene, a small piece of graphene, has attracted unprecedented interest across diverse scientific disciplines particularly in organic electronics. The biological applications nanographenes, such as bioimaging, cancer therapies and drug delivery, provide significant opportunities for breakthroughs the field. However, intrinsic aggregation behavior low solubility which stem from their flat structures, hamper development bioapplications. Herein, we report water‐soluble warped...
Phospha-fluorescein (POF), a phosphine oxide-containing analogue of fluorescein, was synthesized and its photophysical properties were examined. Compared with fluorescein sila-fluorescein, POF displayed significantly red-shifted absorption fluorescence as well superior photobleaching resistance, while retaining the pH-responsive characteristics dyes.
A series of tetracationic quadrupolar chromophores containing three-coordinate boron π-acceptors linked by different π-bridges, namely 4,4'-biphenyl, 2,7-pyrene, 2,7-fluorene, 3,6-carbazole and 5,5'-di(thien-2-yl)-3,6-diketopyrrolopyrrole, were synthesized. While their neutral precursors 1-5 displayed highly solvatochromic fluorescence, the water-soluble target molecules 1M-5M, did not, but emission colour could be tuned from blue to pink changing π-bridge. Compound 5M, diketopyrrolopyrrole...
Abstract Metabolic distribution of fatty acid to organelles is an essential biological process for energy homeostasis as well the maintenance membrane integrity, and metabolic pathways are strictly regulated in response environmental stimuli. Herein, we report a fluorescent probe, which bears azapyrene dye that changes its absorption emission features depending on microenvironment polarity organelle into it transported. Owing sensitivity this dye, metabolically incorporated probe non-polar...
Plasma membrane damage (PMD) occurs in all cell types due to environmental perturbation and cell-autonomous activities. However, cellular outcomes of PMD remain largely unknown except for recovery or death. In this study, using budding yeast normal human fibroblasts, we found that senescence-stable cycle arrest contributing organismal aging-is the long-term outcome PMD. Our genetic screening unexpectedly identified a close association between response replicative lifespan regulations....
Abstract Phototropin is the blue-light receptor that mediates phototropism, chloroplast movement, and stomatal opening in Arabidopsis. Blue red light induce movement moss Physcomitrella patens. To study photoreceptors for P. patens, four phototropin genes (PHOTA1, PHOTA2, PHOTB1, PHOTB2) were isolated by screening cDNA libraries. These classified into two groups (PHOTA PHOTB) on basis of their deduced amino acid sequences. Then disruptants generated homologous recombination used analysis...
During regeneration, differentiated plant cells can be reprogrammed to produce stem cells, a process that requires coordination of cell cycle reactivation with acquisition other cellular characteristics. However, the factors coordinate two functions during reprogramming have not been determined. Here, we report link between and new cell-type characteristics through activity cyclin-dependent kinase A (CDKA) in moss Physcomitrella patens. Excised gametophore leaf P. patens are readily...
Abstract Microtubules form arrays with parallel and antiparallel bundles function in various cellular processes, including subcellular transport cell division. The phragmoplasts, plant-unique microtubule arrays, are mostly unexplored potentially offer new insights. Here, we report that the Physcomitrella patens kinesins KINID1a KINID1b (for kinesin for interdigitated microtubules 1a 1b), which specific to land plants orthologous Arabidopsis thaliana PAKRP2, novel factors indispensable...
Inducible transgene expression provides a useful tool to analyze gene function. The moss Physcomitrella patens is model basal land plant with well-developed research tools, including high efficiency of targeting and substantial genomics resources. However, current systems for controlled remain limited. Here we report the development an estrogen receptor mediated inducible system, based on system used in flowering plants. After identifying appropriate promoters drive chimeric transducer,...
Abstract Bright fluorescent molecules with long fluorescence lifetimes are important for the development of lifetime‐based imaging techniques. Herein, a molecular design is described simultaneously attaining lifetime ( τ ) and high brightness Φ F × ɛ in system that features macrocyclic dimerization π‐conjugated skeletons flexible linkers. An alkylene‐linked dimer bis(thienylethynyl)anthracene was found to show excimer emission ≈19 ns) solution, while maintaining brightness. A comparison...