A5077494119- Microbial infections and disease research
- Synthesis and characterization of novel inorganic/organometallic compounds
- Herpesvirus Infections and Treatments
- Mosquito-borne diseases and control
- Radical Photochemical Reactions
- Mitochondrial Function and Pathology
- Airway Management and Intubation Techniques
- Animal Virus Infections Studies
- Genetics, Aging, and Longevity in Model Organisms
- Neurogenesis and neuroplasticity mechanisms
- Synthetic Organic Chemistry Methods
- Ubiquitin and proteasome pathways
- Autophagy in Disease and Therapy
- Aquaculture disease management and microbiota
- Mycotoxins in Agriculture and Food
- Photosynthetic Processes and Mechanisms
- Reproductive tract infections research
- Neuroinflammation and Neurodegeneration Mechanisms
- Genetic and Kidney Cyst Diseases
- Slime Mold and Myxomycetes Research
- Medical Imaging and Pathology Studies
- Sulfur-Based Synthesis Techniques
- Neuroscience and Neuropharmacology Research
- Preterm Birth and Chorioamnionitis
- Williams Syndrome Research
Gunma University
2021-2025
Japanese Red Cross Kumamoto Hospital
2020-2022
Nagoya University
2015-2017
Tokushima University
2010
Tohoku University
2007
Shokei Gakuin University
2007
National Institutes of Health
2005-2007
Iwate University
2004
National Institute of Infectious Diseases
1988-2000
National Institute of Health Sciences
1983-1998
Abstract Electron‐donating aryl groups were attached to electron‐accepting benzophosphole skeletons. Among several derivatives thus prepared, one oxide was particularly interesting, as it retained high fluorescence quantum yields even in polar and protic solvents. This phosphole‐based compound exhibited a drastic color change of its spectrum function the solvent polarity, while absorption spectra remained virtually unchanged. Capitalizing on these features, this used stain adipocytes, which...
Physarum polycephalum is a well-studied microbial eukaryote with unique experimental attributes relative to other model organisms. It has sophisticated life cycle several distinct stages including amoebal, flagellated, and plasmodial cells. unusual in switching between open closed mitosis according specific life-cycle stages. Here we present the analysis of genome this enigmatic important organism compare it closely related species. The littered simple complex repeats coding regions are...
Two hundred fifty strains of Mycoplasma pneumoniae isolated during the past 20 years in Japan were classified into two groups (I and II) based upon different PCR-restriction fragment length polymorphism patterns their P1 cytadhesin genes. Clear shifts between M. observed but did not appear to be correlated with epidemic cycles. Patients' sera showed relatively higher levels antiadhesin antibodies homologous infecting strain.
Abstract Mitochondrial DNA (mtDNA) is organized in nucleoprotein complexes called mitochondrial nucleoids (mt-nucleoids), which are critical units of mtDNA replication and transmission. In humans, several hundreds mt-nucleoids exist a cell. However, how numerous maintained during the cell cycle remains elusive, because synchronization procedures affect replication. Here, we analyzed regulation maintenance cycle, using fluorescent indicator, Fucci2. Live imaging with higher temporal...
Abstract Electron‐donating aryl groups were attached to electron‐accepting benzophosphole skeletons. Among several derivatives thus prepared, one oxide was particularly interesting, as it retained high fluorescence quantum yields even in polar and protic solvents. This phosphole‐based compound exhibited a drastic color change of its spectrum function the solvent polarity, while absorption spectra remained virtually unchanged. Capitalizing on these features, this used stain adipocytes, which...
Mitochondrial DNA (mtDNA) is organized with proteins into mitochondrial nucleoid (mt-nucleoid). The mt-nucleoid a unit for the maintenance and function of mtDNA. regulator chromosome condensation 1-like protein (RCC1L) performs various functions in mitochondria, including translation, but its involvement regulating unknown. Herein, we found that human RCC1L was required to maintain mt-nucleoids Human has three splicing isoforms: RCC1LV1, RCC1LV2, RCC1LV3. Knockout (KO) cells lacking all...
Abstract In many sexually reproducing organisms, oocytes are fundamentally fertilized with one sperm. Caenorhabditis elegans , chitin layer formation after fertilization by the EGG complex is of mechanisms polyspermy block, but other remain unknown. Here, we demonstrate that MARC-3, a membrane-associated RING-CH-type ubiquitin ligase localizes to plasma membrane and cortical puncta in oocytes, involved fast block. During polyspermy, second sperm entry occurs within approximately 10 s...
Abstract Unsymmetrical cyanine dyes, such as thiazole orange, are useful for the detection of nucleic acids with fluorescence because they dramatically enhance upon binding to acids. Herein, we synthesized a series unsymmetrical dyes and evaluated their properties. A systematic structure–property relationship study has revealed that dialkylamino group at 2‐position quinoline in plays critical role enhancement. Four newly designed showed negligible intrinsic free state strong double‐stranded...
Allophagy is responsible for the selective removal of paternally inherited organelles, including mitochondria, in Caenorhabditis elegans embryos, thereby facilitating maternal inheritance mitochondrial DNA. We previously identified two key factors allophagy: an autophagy adaptor allophagy-1 (ALLO-1) and TBK1/IKKε family kinase IKKE-1. However, precise mechanisms by which ALLO-1 IKKE-1 regulate local autophagosome formation remain unclear. In this study, we identify isoforms with different...
Mycoplasma fermentans incognitus has attracted much interest either as a cofactor for the progression of AIDS or pathogenic agent in non-AIDS-related diseases (S.-C. Lo, M. S. Dawson, P. B. Newton III, A. Sonoda, J. W.-K. Shih, W. F. Engler, R. Y.-H. Wang, and D. Wear, Am. Trop. Med. Hyg. 41:364-376, 1989; S.-C. Wong, R.Y.-H H. Alter, 41:601-616, J.W.-K. N.-Y. Yang, C.-Y. Ou, 40:213-226, 1989). In present study, genetic serologic properties strain other strains were compared. Furthermore,...
Mammalian syntaxin 17 (Stx17) has several roles in processes other than membrane fusion, including mitochondrial division, autophagosome formation and lipid droplet expansion. In contrast to conventional syntaxins, Stx17 a long C-terminal hydrophobic region with hairpin-like structure flanked by basic amino acid-enriched tail. Although is one of the six ancient SNAREs present diverse eukaryotic organisms, it been lost multiple lineages during evolution. study, we compared localization...
WBSCR16 (Williams-Beuren Syndrome Chromosomal Region 16) gene is located in a large deletion region of Williams-Beuren syndrome (WBS), which neurodevelopmental disorder. Although the relationship between and WBS remains unclear, it has been reported that member functional module regulates mitochondrial 16S rRNA abundance intra-mitochondrial translation. RCC1 (Regulator Chromosome Condensation 1)-like amino acid sequence repeats but function appears to be different from other superfamily...
It was demonstrated that mycoplasmas, gram-negative bacteria, and certain gram-positive bacteria share a similar protein antigen with molecular weight ranging from 42,000 to 48,000. Western blotting (immunoblotting) an antibody specific 43-kDa membrane of Mycoplasma fermentans showed the existence this in all spp. tested (14 species), Acholeplasma laidlawii (1 strain), (8 species) but only Staphylococcus aureus four species tested. Neither Ureaplasma urealyticum nor mammalian cell cultures...
When wood based panels are nailed and/or bonded to lumber frame, effects of mechanical restraint on the panel hygroscopic linear expansion (LE) sometimes differ at in-plane biaxial directions. Even adverse effect that LE in one direction becomes larger than its free is recognized when other strongly restrained. To give a better understanding for this phenomenon, study was designed investigate "Poisson's effect" by comparing and LEs uniaxially restrained specimen medium density fiberboard...
Because several reports have suggested that bacterial vaginosis causes premature labour and early rupture of the fetal membranes, presence a flora is thought to be risk factor for labour. The present study investigated two patients with delivery intra-uterine Mycoplasma hominis infection. In microbiological studies, Gram’s staining amniotic fluids revealed numerous neutrophils epithelial cells but no micro-organisms. Culture fluid before antibiotic therapy yielded only M. under anaerobic...
Chicken egg yolk broth medium containing 10% (vol/vol) phosphate-buffered saline extract of instead horse serum showed better growth-promoting activity for Mycoplasma pneumoniae than did Chanock and was effective in promoting the growth small numbers M. pneumoniae. Moreover, proved superior to following respects: (i) it consistent among different batches; (ii) could be preserved at 4 degrees C least 2 years; (iii) is inexpensive easy obtain; (iv) contains large amounts lipoproteins,...
法 で ある点 か ら Rapid determination system f 。r viral sequences
新しいCV測定法であるブタノール法を魚油および食品抽出油脂の測定にはじめて応用した.魚油の測定では,ベンゼン法との相関性を認めると共に,ベンゼン法では測定できないPVの高い試料や,酸化初期の微量なCVの増加量を,正確に測定できることが示された.また,食品抽出油脂の測定においては,試料量が少なくても測定でき,サンプルの色が測定値に影響を与えることがなかった.本法は,操作が簡便で精度が高く,かつ測定用溶剤の毒性が低く使用量も少なくてよいことから,食品製造現場などでの測定にも適しており,魚油や食品の酸化劣化度を評価するために有効な方法であると考えられる.
Airway management in children with severe burns is difficult because of airway edema and prolonged duration ventilatory management. There insufficient evidence to suggest that tracheostomy beneficial for children.A male child aged 1 year 4 months was injured when he accidentally fell into a bathtub filled boiling water. Furthermore, 85% the burnt area, including face neck, consisted second-degree burns; hence, oral tracheal intubation resuscitative infusion were required. In this case,...