A5061980287- Cellular transport and secretion
- Genetics, Aging, and Longevity in Model Organisms
- Autophagy in Disease and Therapy
- Endoplasmic Reticulum Stress and Disease
- Fungal and yeast genetics research
- Mitochondrial Function and Pathology
- Immune Cell Function and Interaction
- Photosynthetic Processes and Mechanisms
- Retinal Development and Disorders
- Balance, Gait, and Falls Prevention
- Cancer Immunotherapy and Biomarkers
- CAR-T cell therapy research
- Pancreatic function and diabetes
- Microtubule and mitosis dynamics
- Plant responses to water stress
- Pancreatic and Hepatic Oncology Research
- Stroke Rehabilitation and Recovery
- Musculoskeletal pain and rehabilitation
- Hippo pathway signaling and YAP/TAZ
- Inorganic and Organometallic Chemistry
- Renal Diseases and Glomerulopathies
- Retinoids in leukemia and cellular processes
- Garlic and Onion Studies
- Anesthesia and Sedative Agents
- Ginger and Zingiberaceae research
Niigata University
2006-2025
Gunma University
2015-2024
National Institute for Environmental Studies
2022-2024
Niigata University Medical and Dental Hospital
2018-2021
Tokyo Metropolitan Geriatric Hospital
2020
Hiroshima University
2018
Kanagawa Institute of Technology
2018
Tokyo Metropolitan University
2018
Gunma University Hospital
2016
Tokyo University of Agriculture
2015
Maternal inheritance of mitochondrial DNA results from autophagy-dependent clearance paternal mitochondria.
Immunization of BALB/c mice with alum-adsorbed OVA, followed by three bronchoprovocations aerosolized resulted in the development airway hyperresponsiveness (AHR) and allergic inflammation lung accompanied severe infiltration eosinophils into airways. In this murine asthma model, administration monoclonal anti-IL-5 Ab before each Ag challenge markedly inhibited eosinophilia, but treatment did not affect AHR. aerosol challenges OVA following same protocol failed to induce AHR mast...
As an approach to understand the molecular mechanisms of endoplasmic reticulum (ER) protein sorting, we have isolated yeast rer mutants that mislocalize a Sec12-Mfalpha1p fusion from ER later compartments secretory pathway (S. Nishikawa and A. Nakano, Proc. Natl. Acad. Sci. USA 90:8179-8183, 1993). The temperature-sensitive rer2 mutant mislocalizes different types membrane proteins, suggesting RER2 is involved in correct localization proteins general. shows several other characteristic...
Rer1p, a yeast Golgi membrane protein, is required for the retrieval of set endoplasmic reticulum (ER) proteins. We present first evidence that Rer1p directly interacts with transmembrane domain (TMD) Sec12p which contains signal. A green fluorescent protein (GFP) fusion rapidly cycles between and ER. Either lesion coatomer or deletion COOH-terminal tail causes its mislocalization to vacuole. The in vitro complex containing alpha gamma subunits. These findings not only give proof novel type...
Yeast Sec12p is a type II transmembrane protein in the ER, which essential for formation of transport vesicles. From biochemical and morphological lines evidence, we have proposed that localized to ER by two mechanisms: static retention dynamic retrieval from early Golgi compartment. We also shown Rer1p, membrane Golgi, required correct localization Sec12p. In present study, performed systematic analysis determine signals corresponding these mechanisms. Both domain (TMD) NH2-terminal...
Caveolin is the major protein component required for formation of caveolae on plasma membrane. Here we show that trafficking Caenorhabditis elegans caveolin-1 (CAV-1) dynamically regulated during development germ line and embryo. In oocytes a CAV-1-green fluorescent (GFP) fusion found membrane in large vesicles (CAV-1 bodies). After ovulation fertilization CAV-1 bodies fuse with manner reminiscent cortical granule exocytosis as described other species. Fusion appears to be by advancing cell...
Maternal inheritance of mitochondrial DNA (mtDNA) is generally observed in many eukaryotes. Sperm-derived paternal mitochondria and their mtDNA enter the oocyte cytoplasm upon fertilization then normally disappear during early embryogenesis. However, mechanism underlying this clearance has remained largely unknown. Recently, we showed that autophagy required for elimination Caenorhabditis elegans embryos. Shortly after fertilization, autophagosomes are induced locally around penetrated sperm...
Lipoproteins regulate the overall lipid homeostasis in animals. However, molecular mechanisms underlying lipoprotein trafficking remain poorly understood. Here, we show that SFT-4, a Caenorhabditis elegans homologue of yeast Erv29p, is essential for endoplasmic reticulum (ER) export yolk protein VIT-2, which synthesized as complex. SFT-4 loss strongly inhibits ER exit proteins and certain soluble cargo intestinal cells. predominantly localizes at sites (ERES) physically interacts with VIT-2...
Rer1p, a Golgi membrane protein, is required for the correct localization of an endoplasmic reticulum (ER) Sec12p, by retrieval mechanism from cis -Golgi to ER. To test whether or not role Rer1p common multiple ER proteins, we examined two other Sec71p and Sec63p, in wild-type rer1 mutant yeast cells, using their fusions with α-mating factor precursor (Mfα1p). Although Sec63p have completely different topology Mfα1p fusion proteins were also mislocalized trans mutant. Overexpression these...
The yeast Golgi membrane protein Rer1p is required for the retrieval of various endoplasmic reticulum (ER) proteins such as Sec12p and Sec71p to ER. We demonstrate here that transmembrane domain (TMD) Sec71p, a type-III protein, contains an ER localization signal, which physical recognition by Rer1p. Sec71TMD-GFP fusion efficiently retrieved structural feature this TMD signal turns out be spatial location polar residues flanking highly hydrophobic core sequence but not whole length TMD. On...
We previously identified a novel type of caveolin-enriched secretory vesicle in Caenorhabditis elegans oocytes. These vesicles undergo synchronous fusion with the plasma membrane immediately after fertilization, suggesting that they could be cortical granules have been described diverse animal species. Here, we report these are indeed granules, delivering essential chondroitin proteoglycans and mucin-like glycoproteins to early embryonic extracellular matrices (ECMs). Furthermore, found...
Clathrin is a coat protein involved in vesicle budding from several membrane-bound compartments within the cell. Here we present an analysis of temperature-sensitive (ts) mutant clathrin heavy chain (CHC) multicellular animal. As expected Caenorhabditis elegans chc-1(b1025ts) animals are defective receptor-mediated endocytosis and arrest development soon after being shifted to restrictive temperature. Steady-state levels these mutants reduced by more than 95% at all temperatures. Hub...
Intestinal epithelial cells have unique apical membrane structures, known as microvilli, that contain bundles of actin microfilaments. In this study, we report Caenorhabditis elegans cytosolic chaperonin containing TCP-1 (CCT) is essential for proper formation microvilli in intestinal cells. cct-5(RNAi) animals, a substantial amount lost from the area, forming large aggregates cytoplasm, and deformed into abnormal, bubble-like structures. The length decreased these animals. However, overall...
[Purpose] To evaluate the association between foot sole two-point discrimination and dynamic standing balance. [Participants Methods] This cross-sectional, observational study included 50 healthy adults. Participants were made to stand on a firm or foam surface with eyes open closed, center of pressure length was measured for static balance limits stability Two-point muscle strength assessed using test toe grip strength, respectively. We then analyzed sensory-motor assessment [Results]...
In recent clinical practice, driver gene mutations have been tested using multiplex PCR or next-generation sequencing (NGS), which help determine treatment strategies for non-small cell lung cancer (NSCLC). We developed a new analysis system, the Mutation Investigator Next-era Sequencer (MINtS), NGS, allows detection of even in cytology specimens with low tumor content. Due to its high sensitivity, MINtS has potential detect that are pathologically negative cancer. present study, we examined...