A5083723977- Legionella and Acanthamoeba research
- Cellular transport and secretion
- Endoplasmic Reticulum Stress and Disease
- Autophagy in Disease and Therapy
- Biomedical Research and Pathophysiology
- Microtubule and mitosis dynamics
- Heme Oxygenase-1 and Carbon Monoxide
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Lipid Membrane Structure and Behavior
- Mitochondrial Function and Pathology
- Lipid metabolism and biosynthesis
- Erythrocyte Function and Pathophysiology
- Vibrio bacteria research studies
- Photosynthetic Processes and Mechanisms
- Ubiquitin and proteasome pathways
- Biochemical and Structural Characterization
- Calcium signaling and nucleotide metabolism
- Retinal Development and Disorders
- Neonatal Health and Biochemistry
- Plant biochemistry and biosynthesis
- Protist diversity and phylogeny
- Poxvirus research and outbreaks
- Signaling Pathways in Disease
- ATP Synthase and ATPases Research
- Cell Image Analysis Techniques
Tokyo University of Pharmacy and Life Sciences
2015-2025
Gifu University
2023
Yale University
2009-2018
National Institute of Technology, Tokyo College
2018
Life University
2017
In 2008, we published the first set of guidelines for standardizing research in autophagy. Since then, this topic has received increasing attention, and many scientists have entered field. Our knowledge base relevant new technologies also been expanding. Thus, it is important to formulate on a regular basis updated monitoring autophagy different organisms. Despite numerous reviews, there continues be confusion regarding acceptable methods evaluate autophagy, especially multicellular...
During infection of macrophages, the pathogenic bacterium Legionella pneumophila secretes effector proteins that induce conversion plasma membrane-derived vacuole into an endoplasmic reticulum (ER)-like replicative vacuole. These ER-like vacuoles are ultimately fused with ER, where pathogen replicates. Here we show L. Lpg1137 is a serine protease targets mitochondria and their associated membranes. binds to cleaves syntaxin 17, soluble N-ethylmaleimide-sensitive factor attachment protein...
The microtubule-associated protein MAP1B has been implicated in axonal growth and brain development. We found that is highly expressed the most aggressive deadliest breast cancer subtype, triple-negative (TNBC), but not other subtypes. Expression of was to be correlated with poor prognosis. Depletion TNBC cells impairs cell migration invasion concomitant a defect tumorigenesis. interacts key components for invadopodia formation, cortactin, Tks5, latter which PtdIns(3,4)P2-binding scaffold...
Biogenesis of a specialized organelle that supports intracellular replication Legionella pneumophila involves the fusion secretory vesicles exiting endoplasmic reticulum (ER) with phagosomes containing this bacterial pathogen. Here, we investigated host plasma membrane SNARE proteins to determine whether they play role in trafficking vacuoles L. pneumophila. Depletion syntaxins by RNA interference resulted delayed acquisition resident ER protein calnexin and enhanced retention Rab1 on...
The Dot/Icm system of the intracellular pathogen Legionella pneumophila has capacity to deliver over 270 effector proteins into host cells during infection. Important questions remain as spatial and temporal mechanisms used regulate such a large array virulence determinants after they have been delivered cells. Here we investigated several L. that contain conserved phosphatidylinositol-4-phosphate (PI4P)-binding domain first described in DrrA (SidM). This PI4P binding was essential for...
Vesicle-associated membrane protein-associated protein (VAP) is an endoplasmic reticulum (ER)-resident integral that controls a nonvesicular mode of ceramide and cholesterol transfer from the ER to Golgi complex by interacting with oxysterol-binding (OSBP), respectively. We report VAP its proteins are required for processing secretion pancreatic adenocarcinoma up-regulated factor, whose transport trans-Golgi network (TGN) cell surface mediated carriers called "carriers surface" (CARTS). In...
Legionella pneumophila is an intracellular bacterial pathogen that modulates membrane trafficking to survive and proliferate within host cells. After phagocytosis, the L. pneumophila–containing vacuole evades endocytic pathway by excluding GTPase Rab5, a crucial regulator of phagosomal maturation. In this study, we show evolutionarily conserved lysine residue K134 Rab5 undergoes ubiquitination during infection. This modification depends on Lpg2525, F-box protein from acts as component...
RINT-1 was first identified as a Rad50-interacting protein that participates in radiation-induced G2/M checkpoint control. We have recently reported RINT-1, together with the dynamitin-interacting ZW10 and others, is associated syntaxin 18, an endoplasmic reticulum (ER)-localized SNARE involved membrane trafficking between ER Golgi. To address role of trafficking, we examined effects overexpression knockdown on Golgi morphology transport from ER. Overexpression N-terminal region which...
In response to cholesterol deprivation, SCAP escorts SREBP transcription factors from the endoplasmic reticulum Golgi complex for their proteolytic activation, leading gene expression synthesis and uptake. Here, we show that in cholesterol-fed cells, ER-localized interacts through Sac1 phosphatidylinositol 4-phosphate (PI4P) phosphatase with a VAP–OSBP complex, which mediates counter-transport of ER PI4P at ER–Golgi membrane contact sites (MCSs). knockdown inhibited turnover PI4P, perhaps...
Legionella pneumophila ( L . ) is a gram-negative bacterium that replicates in compartment resembles the host endoplasmic reticulum (ER). To create its replicative niche, manipulates membrane traffic and fusion machineries. Bacterial proteins called effectors are translocated into cytosol play crucial role these processes. In an early stage of infection, subverts ER-derived vesicles (ERDVs) by manipulating GTPase Rab1 to facilitate remodeling -containing vacuole (LCV). Subsequently, LCV...
The presence of subdomains in the endoplasmic reticulum (ER) enables this organelle to perform a variety functions, yet mechanisms underlying their organization are poorly understood. In present study, we show that syntaxin 18, SNAP (soluble NSF attachment protein) receptor localized ER, is important for two ER subdomains, smooth/rough membranes and exit sites. Knockdown 18 caused global change membrane architecture, leading segregation smooth rough ER. Furthermore, sites was markedly...
Lipid droplets (LDs) are ubiquitous organelles that contain neutral lipids and surrounded by a phospholipid monolayer. How proteins specifically localize to the monolayer of LD surface has been matter extensive investigations. In present study, we show syntaxin 17 (Stx17), soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) whose expression in liver is regulated diet, participates biogenesis regulating distribution acyl-CoA synthetase (ACSL)3, key enzyme for...
The intracellular bacterial pathogen Legionella pneumophila uses many effector proteins delivered by the type IV secretion system (T4SS) to hijack early secretory pathway establish its replicative niche, known as Legionella-containing vacuole (LCV). On LCV biogenesis, endoplasmic reticulum (ER) vesicular soluble N-ethylmaleimide-sensitive factor attachment protein receptors (v-SNARE) Sec22b is recruited phagosome and forms non-canonical pairings with target membrane SNAREs (t-SNAREs) from...
The SNARE protein syntaxin 5 exists as long (42 kDa) and short (35 isoforms. form is principally localized in the Golgi complex, whereas resides not only but also endoplasmic reticulum (ER). Although Golgi-localized has been extensively investigated, little known about form. In present study, we demonstrate that of functions to shape ER. We found overexpression induces rearrangement co-alignment ER membrane with microtubules, pattern which quite similar observed cells overexpressing...
Metastasis is a major cause of cancer-related death. Membrane type 1–matrix metalloproteinase (MT1-MMP) critical protease for local invasion and metastasis. MT1-MMP synthesized in the endoplasmic reticulum (ER) transported vesicles to invadopodia, specialized subdomains plasma membrane, through secretory endocytic recycling pathways. The molecular mechanism underlying intracellular transport has been extensively studied, but not fully understood. We show that diverts SNARE Bet1 from its...
Pathogens subvert host defense systems including autophagy and apoptosis for their survival proliferation. Legionella pneumophila is a Gram-negative bacterium that grows in alveolar macrophages causes severe pneumonia. Early during infection secretes effector proteins convert the plasma membrane-derived vacuole containing into an endoplasmic reticulum (ER)-like replicative vacuole. These vacuoles ultimately fuse with ER, where pathogen replicates. Recently, we showed one of effectors,...
During the initial stage of infection, Legionella pneumophila secretes effectors that promote fusion endoplasmic reticulum (ER)–derived vesicles with Legionella-containing vacuole (LCV). This leads to a remodeling plasma membrane (PM)–derived LCV into specialized ER-like compartment supports bacterial replication. Although effector DrrA has been shown activate small GTPase Rab1, it remains unclear how promotes tethering host LCV. Here, we show Sec5, Sec15, and perhaps Sec6, which are...