A5037191167- Plant Molecular Biology Research
- Plant nutrient uptake and metabolism
- Plant Reproductive Biology
- Plant Stress Responses and Tolerance
- Plant Micronutrient Interactions and Effects
- Plant tissue culture and regeneration
- Genomics, phytochemicals, and oxidative stress
- Parathyroid Disorders and Treatments
- Legume Nitrogen Fixing Symbiosis
- Photosynthetic Processes and Mechanisms
- Autophagy in Disease and Therapy
- Pharmacogenetics and Drug Metabolism
- Plant Gene Expression Analysis
- Transgenic Plants and Applications
- Endoplasmic Reticulum Stress and Disease
- Light effects on plants
- Hormonal Regulation and Hypertension
- Plant-Microbe Interactions and Immunity
- Aluminum toxicity and tolerance in plants and animals
- Iron oxide chemistry and applications
- Moringa oleifera research and applications
- Drug Transport and Resistance Mechanisms
- Plant Virus Research Studies
- Metabolomics and Mass Spectrometry Studies
- Plant biochemistry and biosynthesis
Leibniz Institute of Plant Biochemistry
2014-2024
Martin Luther University Halle-Wittenberg
2001-2024
University of California, Davis
2007-2024
University of California, Riverside
2024
Leibniz Association
2011-2021
Signal Processing (United States)
2020
University of Tennessee at Knoxville
2018
Plant (United States)
2005
U.S. Vegetable Laboratory
2000
Plant Gene Expression Center
1992-1998
In 2008, we published the first set of guidelines for standardizing research in autophagy. Since then, this topic has received increasing attention, and many scientists have entered field. Our knowledge base relevant new technologies also been expanding. Thus, it is important to formulate on a regular basis updated monitoring autophagy different organisms. Despite numerous reviews, there continues be confusion regarding acceptable methods evaluate autophagy, especially multicellular...
The plant hormone IAA (or auxin) is central to the control of growth and development. Processes governed by auxin in concert with other regulators include development vascular tissues, formation lateral adventitious roots, apical dominance, tropic responses (Went Thimann, 1937). At level cellular physiology, profoundly affects turgor, elongation, division, cell differentiation, major driving shaping forces morphogenesis oncogenesis. molecular mechanisms action are still unknown, although it...
The plant growth hormone indoleacetic acid (IAA) transcriptionally activates gene expression in plants. Some of the genes whose is induced by IAA encode a family proteins pea (PS-IAA4 and PS-IAA6) Arabidopsis (IAA1 IAA2) that contain putative nuclear localization signals direct beta-glucuronidase reporter protein into nucleus. Pulse-chase immunoprecipitation experiments have defined t1/2 PS-IAA4 PS-IAA6 to be 8 6 min, respectively. Their most prominent feature presence beta alpha motif...
Summary An improved protocol is reported to isolate and transiently transform mesophyll protoplasts of Arabidopsis thaliana. Transfected leaf support high levels expression the bacterial reporter gene coding for β‐glucuronidase (GUS), under control cauliflower mosaic virus (CaMV) 35S promoter. Transient GUS activity was monitored spectrophotometrically reached a maximum between 18 48 h after polyethylene glycol (PEG)‐mediated DNA uptake. Histochemical staining revealed reproducible...
Abstract Environmental cues profoundly modulate cell proliferation and elongation to inform direct plant growth development. External phosphate (Pi) limitation inhibits primary root in many species. However, the underlying Pi sensory mechanisms are unknown. Here we genetically uncouple two sensing pathways apex of Arabidopsis thaliana . First, rapid inhibition transition zone is controlled by transcription factor STOP1, its target, ALMT1 , encoding a malate channel, ferroxidase LPR1, which...
Eukaryotes have evolved various quality control mechanisms to promote proteostasis in the endoplasmic reticulum (ER). Selective removal of certain ER domains via autophagy (termed as ER-phagy) has emerged a major mechanism. However, degree which ER-phagy is employed by other branches ER-quality remains largely elusive. Here, we identify cytosolic protein, C53, that specifically recruited autophagosomes during ER-stress, both plant and mammalian cells. C53 interacts with ATG8 distinct binding...
Phosphate (Pi) plays a central role as reactant and effector molecule in plant cell metabolism. However, Pi is the least accessible macronutrient many ecosystems its low availability often limits growth. Plants have evolved an array of molecular morphological adaptations to cope with limitation, which include dramatic changes gene expression root development facilitate acquisition recycling. Although physiological responses starvation been increasingly studied understood, initial events that...
Summary Glucosinolates are a class of secondary metabolites with important roles in plant defense and human nutrition. Here, we characterize putative UDP‐glucose:thiohydroximate S ‐glucosyltransferase, UGT74B1, to determine its role the Arabidopsis glucosinolate pathway. Biochemical analyses demonstrate that recombinant UGT74B1 specifically glucosylates thiohydroximate functional group. Low K m values for phenylacetothiohydroximic acid (approximately 6 μ ) UDP‐glucose 50 strongly suggest...
Inadequate availability of inorganic phosphate (Pi) in the rhizosphere is a common challenge to plants, which activate metabolic and developmental responses maximize Pi acquisition. The sensory mechanisms that monitor environmental status regulate root growth via altered meristem activity are unknown. Here, we show PHOSPHATE DEFICIENCY RESPONSE 2 (PDR2) encodes single P(5)-type ATPase Arabidopsis thaliana. PDR2 functions endoplasmic reticulum (ER) required for proper expression SCARECROW...
Summary Plants have evolved complex strategies to maintain phosphate (Pi) homeostasis and maximize Pi acquisition when the macronutrient is limiting. Adjustment of root system architecture via changes in meristem initiation activity integral acclimation process. However, mechanisms that monitor external status interpret nutritional signal remain be elucidated. Here, we present evidence deficiency response , pdr2 mutation disrupts local sensing. The sensitivity amplitude metabolic...
Abstract Auxin/indole-3-acetic acid (Aux/IAA) genes encode short-lived transcription factors that are induced as a primary response to the plant growth hormone IAA or auxin. Gain-of-function mutations in Arabidopsis genes,SHY2/IAA3, AXR3/IAA17, andAXR2/IAA7 cause pleiotropic phenotypes consistent with enhanced auxin responses, possibly by increasing Aux/IAA protein stability. Semidominant shy2-1D,shy2-2, axr3-1, and axr2-1induce ectopic light responses dark-grown seedlings. Because genetic...
1-Aminocyclopropane-1-carboxylic acid (ACC) synthase is the key regulatory enzyme in biosynthetic pathway of plant hormone ethylene. The encoded by a divergent multigene family Arabidopsis thaliana, comprising at least five genes, ACS1-5 (Liang, X., Abel, S., Keller, J. A., Shen, N. F., and Theologis, A.(1992) Proc. Natl. Acad. Sci. U. S. A. 89, 11046-11050). In etiolated seedlings, ACS4 specifically induced indoleacetic (IAA). response to IAA rapid (within 25 min) insensitive protein...
Summary Glucosinolates are a class of secondary metabolites with important roles in plant defense and human nutrition. To uncover regulatory mechanisms glucosinolate production, we screened Arabidopsis thaliana T‐DNA activation‐tagged lines identified high‐glucosinolate mutant caused by overexpression IQD1 ( At3g09710 ). A series gain‐ loss‐of‐function alleles different accessions correlates increased decreased levels, respectively. encodes novel protein that contains putative nuclear...
Genomic sequences encoding five divergent 1-aminocyclopropane-1-carboxylic acid (ACC) synthase polypeptides (ACC1, ACC2, ACC3, ACC4, and ACC5) have been isolated from Arabidopsis thaliana by using heterologous cDNAs PCR fragments amplified genomic DNA with degenerate oligonucleotide primers. Each gene is located on a different chromosome in the genome. The genes are differentially expressed during development response to environmental stimuli. Protein-synthesis inhibition derepresses...
Calcium (Ca2+) signaling and dynamic reorganization of the cytoskeleton are essential processes for coordination control plant cell shape growth. Calmodulin (CaM) closely related calmodulin-like (CML) polypeptides principal sensors Ca2+ signals. CaM/CMLs decode relay information encrypted by second messenger via differential interactions with a wide spectrum targets to modulate their diverse biochemical activities. The plant-specific IQ67 DOMAIN (IQD) family emerged as possibly largest class...
Calcium (Ca(2+)) is a key second messenger in eukaryotes and regulates diverse cellular processes, most notably via calmodulin (CaM). In Arabidopsis thaliana, IQD1 (IQ67 domain 1) the founding member of IQD family putative CaM targets. The 33 predicted proteins share conserved 67 amino acids that characterized by unique arrangement multiple recruitment motifs, including so-called IQ motifs. Whereas has been implicated regulation defense metabolism, biochemical functions remain to be...
Plants have evolved two major strategies to cope with phosphate (Pi) limitation. The systemic response, mainly comprising increased Pi uptake and metabolic adjustments for more efficient use, the local enabling plants explore Pi-rich soil patches by reorganization of root system architecture. Unlike previous reports, this study focused on exudation controlled response deficiency. To approach this, a hydroponic separating responses was developed. Arabidopsis thaliana genotypes exhibiting...
Abstract Background Calcium signaling plays a prominent role in plants for coordinating wide range of developmental processes and responses to environmental cues. Stimulus-specific generation intracellular calcium transients, decoding signatures, transformation the signal into cellular are integral modules transduction process. Several hundred proteins with functions circuits have been identified, number downstream targets sensors is expected increase. We previously identified novel,...
Suspension-cultured cells of tomato (Lycopersicon esculentum) start to secrete an RNA-degrading enzyme activity during transition from logarithmic stationary growth phase. Using affinity chromatography on agarose-5-(4-aminophenyl-phosphoryl) uridine 3'(2') monophosphate as a powerful and final enrichment step, the was purified homogeneity characterized ribonuclease I (RNase I) according following data: (a) it has M(r) 22,000 (sodium dodecyl sulfate-polyacrylamide gel electrophoresis),...