A5052383206- Autophagy in Disease and Therapy
- Endoplasmic Reticulum Stress and Disease
- Occupational Health and Performance
- Injury Epidemiology and Prevention
- Mitochondrial Function and Pathology
- Toxoplasma gondii Research Studies
- Asthma and respiratory diseases
- Cancer, Hypoxia, and Metabolism
- Toxic Organic Pollutants Impact
- Cell death mechanisms and regulation
- RNA modifications and cancer
- Biological Research and Disease Studies
- Inhalation and Respiratory Drug Delivery
- CRISPR and Genetic Engineering
- Fungal and yeast genetics research
- Heavy Metal Exposure and Toxicity
- Berberine and alkaloids research
- Ubiquitin and proteasome pathways
- Nanocomposite Films for Food Packaging
- Adipose Tissue and Metabolism
- S100 Proteins and Annexins
- Genetics, Aging, and Longevity in Model Organisms
- Calcium signaling and nucleotide metabolism
- Pineapple and bromelain studies
- RNA regulation and disease
Polytechnic Institute of Bragança
2024
University of Minho
2010-2024
i3S - Instituto de Investigação e Inovação em Saúde, Universidade do Porto
2018-2022
University of Beira Interior
2018
Universidade do Porto
2018
In 2008, we published the first set of guidelines for standardizing research in autophagy. Since then, this topic has received increasing attention, and many scientists have entered field. Our knowledge base relevant new technologies also been expanding. Thus, it is important to formulate on a regular basis updated monitoring autophagy different organisms. Despite numerous reviews, there continues be confusion regarding acceptable methods evaluate autophagy, especially multicellular...
Colorectal carcinoma (CRC) is one of the most common causes cancer-related mortality. Short-chain fatty acids secreted by dietary propionibacteria from intestine, such as acetate, induce apoptosis in CRC cells and may therefore be relevant prevention therapy. We previously reported that acetic acid-induced Saccharomyces cerevisiae involves partial vacuole permeabilization release Pep4p, yeast cathepsin D (CatD), which has a protective role this process. In cancer cells, lysosomes have...
We have previously shown that acetic acid activates a mitochondria-dependent death process in Saccharomyces cerevisiae and the ADP/ATP carrier (AAC) is required for mitochondrial outer membrane permeabilization cytochrome c release. Mitochondrial fragmentation degradation also been response to this stimulus. Herein, we show autophagy not active cells undergoing acid-induced apoptosis therefore responsible degradation. Furthermore, found vacuolar protease Pep4p AAC proteins role using yeast...
Abstract Acetate is a short-chain fatty acid secreted by Propionibacteria from the human intestine, known to induce mitochondrial apoptotic death in colorectal cancer (CRC) cells. We previously established that acetate also induces lysosome membrane permeabilization CRC cells, associated with release of lysosomal protease cathepsin D (CatD), which has well-established role cascade. Unexpectedly, we showed CatD an antiapoptotic this process, as pepstatin A (a inhibitor) increased...
Colorectal cancer (CRC) is one of the most common malignancies and a leading cause death worldwide. Most cells display high rates glycolysis with production lactic acid, which then exported to microenvironment by monocarboxylate transporters (MCTs). The main aim this study was evaluate significance MCT expression in comprehensive series primary CRC cases, lymph node hepatic metastasis. Expressions MCT1, MCT4, CD147 GLUT1 were studied human samples CRC, metastasis, immunohistochemistry. All...
// Sara Alves 1 , Lisandra Castro Maria Sofia Fernandes 3 Rita Francisco Paula Muriel Priault 2 Susana Rodrigues Chaves Mary Pat Moyer 4 Carla Oliveira Raquel Seruca Manuela Côrte-Real João Sousa 1, * Ana Preto CBMA - Centre of Molecular and Environmental Biology, Department University Minho, Campus de Gualtar, 4710-057 Braga, Portugal CNRS, UMR5095, Bordeaux 2, Bordeaux, France IPATIMUP Institute Pathology Immunology the Porto, Instituto Investigação e...
Available literature has found an association between firefighting and pathologic pathways leading to cardiorespiratory diseases, which have been linked with exposure polycyclic aromatic hydrocarbons (PAHs). PAHs are highlighted as priority pollutants by the European Human Biomonitoring Initiative in occupational non-occupational contexts.
Human biomonitoring data retrieved from real-life wildland firefighting in Europe and, also, worldwide are scarce. Thus, this study, 176 Portuguese firefighters were biomonitored pre- and post- unsimulated wildfire combating (average:12–13 h; maximum: 55 h) to evaluate the impact on levels of urinary polycyclic aromatic hydrocarbons hydroxylated metabolites (OHPAH; quantified by high-performance liquid chromatography with fluorescence detection) associated short-term health effects...
Colorectal cancer (CRC) is a leading cause of death worldwide. Conventional therapies are available with varying effectiveness. Acetate, short-chain fatty acid produced by human intestinal bacteria, triggers mitochondria-mediated apoptosis preferentially in CRC but not normal colonocytes, which has spurred an interest its use for prevention/therapy. We previously uncovered that acetate-induced mitochondrial-mediated cells significantly enhanced the inhibition lysosomal protease cathepsin D...
The plasma membrane (PM) protects cells from extracellular threats and supports cellular homeostasis. Some pathogens produce pore-forming toxins (PFTs) that disrupt PM integrity by forming transmembrane pores. High PFT concentrations cause massive damage leading to cell death facilitating infection. Sub-lytic doses activate repair mechanisms restore integrity, support survival limit disease. Shedding of vesicles (EVs) has been proposed as a key mechanism eliminate pores integrity. We show...
Polysaccharides are an attractive source for use in dressings due to their plentiful availability, low cost, biodegradability, biocompatibility and multifunctionality. In this work we describe the preparation, characterization evaluation...