A5066448163- Sulfur Compounds in Biology
- Plant Pathogens and Fungal Diseases
- Nitrogen and Sulfur Effects on Brassica
- Yeasts and Rust Fungi Studies
- Mycorrhizal Fungi and Plant Interactions
- Autophagy in Disease and Therapy
- Advanced Glycation End Products research
- Selenium in Biological Systems
- RNA Research and Splicing
- Cassava research and cyanide
- Lipid metabolism and biosynthesis
- Polyamine Metabolism and Applications
- RNA modifications and cancer
- Cancer-related molecular mechanisms research
- Redox biology and oxidative stress
- Plant Stress Responses and Tolerance
- Plant responses to water stress
- Genomics, phytochemicals, and oxidative stress
- Soybean genetics and cultivation
- Plant-Microbe Interactions and Immunity
- Diet and metabolism studies
- RNA and protein synthesis mechanisms
- Plant Physiology and Cultivation Studies
- Phytase and its Applications
- Plant tissue culture and regeneration
Universidad de Sevilla
2016-2025
Consejo Superior de Investigaciones Científicas
2011-2025
Instituto de Bioquímica Vegetal y Fotosíntesis
2016-2025
Iowa State University
2020-2021
Centro de Investigaciones Científicas Isla de la Cartuja
2013-2014
Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria
2007-2009
Center for International Forestry Research
2006-2009
In 2008, we published the first set of guidelines for standardizing research in autophagy. Since then, this topic has received increasing attention, and many scientists have entered field. Our knowledge base relevant new technologies also been expanding. Thus, it is important to formulate on a regular basis updated monitoring autophagy different organisms. Despite numerous reviews, there continues be confusion regarding acceptable methods evaluate autophagy, especially multicellular...
Hydrogen sulfide is a highly reactive molecule that currently accepted as signaling compound. This important carbon monoxide in mammals and hydrogen peroxide plants, well nitric oxide both eukaryotic systems. Although many studies have been conducted on the physiological effects of sulfide, underlying mechanisms are poorly understood. One proposed involves posttranslational modification protein cysteine residues, process called S-sulfhydration. In this work, modified biotin switch method was...
Hydrogen sulfide-mediated signaling pathways regulate many physiological and pathophysiological processes in mammalian plant systems. The molecular mechanism by which hydrogen sulfide exerts its action involves the post-translational modification of cysteine residues to form a persulfidated thiol motif, process called protein persulfidation. We have developed comparative quantitative proteomic analysis approach for detection endogenous proteins wild-type Arabidopsis L-CYSTEINE DESULFHYDRASE...
Hydrogen sulfide is an important signaling molecule comparable with nitric oxide and hydrogen peroxide in plants. The underlying mechanism of its action unknown, although it has been proposed to be S-sulfhydration. This post-translational modification converts the thiol groups cysteines within proteins persulfides, resulting functional changes proteins. In Arabidopsis thaliana, S-sulfhydrated have identified, including cytosolic isoforms glyceraldehyde-3-phosphate dehydrogenase GapC1 GapC2....
Hydrogen sulfide is a signaling molecule that regulates essential processes in plants, such as autophagy. In Arabidopsis (Arabidopsis thaliana), hydrogen negatively autophagy independently of reactive oxygen species via an unknown mechanism. Comparative and quantitative proteomic analysis was used to detect abscisic acid-triggered persulfidation reveals main role the control mediated by autophagy-related (ATG) Cys protease AtATG4a. This undergoes specific Cys170 part characteristic catalytic...
Significance Endoplasmic reticulum (ER) stress is a condition triggered by adverse environmental conditions, which in plants include extreme heat, pathogen infection, and drought. These stresses can have devastating effect on agricultural productivity, the appropriate responses of to are therefore critical. Autophagy macromolecular degradation pathway that activated response stress, including ER but mechanism it regulated still unclear. Here, we describe modification, persulfidation, key...
Abstract Hydrogen sulfide (H2S) is a signaling molecule that regulates essential plant processes. In this study, the role of H2S during drought was analysed, focusing on underlying mechanism. Pretreatments with before imposing plants substantially improved characteristic stressed phenotypes under and decreased levels typical biochemical stress markers such as anthocyanin, proline, hydrogen peroxide. also regulated drought-responsive genes amino acid metabolism, repressed drought-induced bulk...
Summary Hydrogen sulfide is a signaling molecule in plants that regulates essential biological processes through protein persulfidation. However, little known about sulfide‐mediated regulation relation to photorespiration. Here, we performed label‐free quantitative proteomic analysis and observed high impact on persulfidation levels when grown under nonphotorespiratory conditions were transferred air, with 98.7% of the identified proteins being more persulfidated suppressed Interestingly,...
Abstract The occurrence of fungal grapevine trunk pathogens associated with nurseries and the decline young vines in Spain was determined extensive surveys conducted vineyards plants. presence Phaeomoniella chlamydospora , Phaeoacremonium aleophilum Cylindrocarpon spp., Botryosphaeria obtusa spp. detected all that were carried out. This study provides evidence for these Spanish nurseries. B. isolated very early planting material production process, nevertheless, P. not until rootstock–scion...
Abstract Hydrogen sulfide regulates essential plant processes, including adaptation responses to stress situations, and the best characterized mechanism of action consists post-translational modification persulfidation. In this study, we reveal first persulfidation proteome described in rice 3443 different persulfidated proteins that participate a broad range biological processes metabolic pathways. addition, comparative proteomics revealed specific involved signaling during drought...
Protein persulfidation is a thiol-based oxidative posttranslational modification (oxiPTM) that involves the of susceptible cysteine thiol groups present in peptides and proteins through hydrogen sulfide (H2S), thus affecting their function. Using sweet pepper (Capsicum annuum L.) fruits as model material at different stages ripening (immature green ripe red), endogenous persulfidated (persulfidome) were labeled using dimedone switch method identified liquid chromatography mass spectrometry...
Persulfidation, a posttranslational modification of cysteines to persulfides, is the best characterized molecular mechanism H2S signaling. This study focused on new functions for thioredoxins (TRXs) in plants beyond those thiol disulfide (S-S) exchange, including regulation protein persulfidation as it has been described animal systems. To elucidate impact TRXo1 deficiency pattern Arabidopsis thaliana L. wild type (WT) and two Attrxo1 T-DNA insertion mutants grown under non stress...