- Plant Stress Responses and Tolerance
- Photosynthetic Processes and Mechanisms
- Peroxisome Proliferator-Activated Receptors
- Nitric Oxide and Endothelin Effects
- Plant Micronutrient Interactions and Effects
- Plant nutrient uptake and metabolism
- Plant responses to water stress
- Plant responses to elevated CO2
- Aluminum toxicity and tolerance in plants and animals
- Postharvest Quality and Shelf Life Management
- Sulfur Compounds in Biology
- Adipose Tissue and Metabolism
- Genomics, phytochemicals, and oxidative stress
- Redox biology and oxidative stress
- Potato Plant Research
- Nitrogen and Sulfur Effects on Brassica
- Recycling and utilization of industrial and municipal waste in materials production
- Lipid metabolism and biosynthesis
- Plant Physiology and Cultivation Studies
- Plant Growth Enhancement Techniques
- Polyamine Metabolism and Applications
- Arsenic contamination and mitigation
- Selenium in Biological Systems
- Silicon Effects in Agriculture
- Antioxidant Activity and Oxidative Stress
Estación Experimental del Zaidín
2016-2025
Consejo Superior de Investigaciones Científicas
2016-2025
National Research Council
2007-2022
Institute of Agrifood Research and Technology
2021
University of Edinburgh
2019
Universidad de Jaén
2000-2018
Center of Biotechnogy of Borj Cédria
2016
Unidades Centrales Científico-Técnicas
1998-2014
Instituto de Biología Molecular y Celular de Plantas
1997-2009
National Institute for Basic Biology
2009
ABSTRACT Cadmium is a toxic metal that produces disturbances in plant antioxidant defences giving rise to oxidative stress. The effect of this on H 2 O and ·− production was studied leaves from pea plants growth for weeks with 50 µ m Cd, by histochemistry diaminobenzidine (DAB) nitroblue tetrazolium (NBT), respectively. subcellular localization these reactive oxygen species (ROS) cytochemistry CeCl 3 Mn/DAB staining , respectively, followed electron microscopy observation. In grown CdCl six...
Peroxisomes can be broadly defined as subcellular organelles bounded by a single membrane that contain basic enzymatic constituents catalase and hydrogen peroxide (H2O2)-producing flavin oxidases occur in almost all eukaryotic cells ([Baker Graham, 2002][1]). In recent years, it has
ABSTRACT Growth of pea ( Pisum sativum L.) plants with 50 µ m CdCl 2 for 15 d produced a reduction in the number and length lateral roots, changes structure principal roots affecting xylem vessels. Cadmium induced glutathione (GSH) ascorbate (ASC) contents, catalase (CAT), GSH reductase (GR) guaiacol peroxidase (GPX) activities. CuZn‐superoxide dismutase (SOD) activity was also diminished by Cd treatment, although Mn‐SOD slightly increased. CAT CuZn‐SOD were down‐regulated at transcriptional...
Senescence is a genetically regulated oxidative process that involves general degradation of the cellular structures and enzymes mobilization products to other parts plant. mainly characterized by cessation photosynthesis, disintegration
Abstract The cellular and subcellular localization of endogenous nitric oxide (NO ˙) in leaves from young senescent pea (Pisum sativum) plants was studied. Confocal laser scanning microscopy analysis leaf sections with the fluorescent probe 4,5-diaminofluorescein diacetate revealed that NO ˙ mainly present vascular tissues (xylem phloem). Green fluorescence spots were also detected epidermal cells, palisade spongy mesophyll guard cells. In leaves, generation clearly reduced tissues. At...
The effect in vivo of salt stress on the activated oxygen metabolism mitochondria, was studied leaves from two NaCl‐treated cultivars Pisum sativum L. with different sensitivity to NaCl. In mitochondria NaCl‐sensitive plants, salinity brought about a significant decrease Mn‐SOD (EC 1. 15. 1) Cu, Zn‐SOD I and fumarase 4. 2. 2) activities. Conversely, salt‐tolerant plants NaCl treatment produced an increase mitochondrial activity and, lesser extent, activity. both salt‐treated cultivars,...
Nitrosative stress has become a usual term in the physiology of nitric oxide mammalian systems. However, plants there is much less information on this type stress. Using olive leaves as experimental model, effect salinity potential induction nitrosative was studied. The enzymatic l ‐arginine‐dependent production (NOS activity) measured by ozone chemiluminiscence. specific activity NOS 0.280 nmol NO mg −1 protein min , and dependent ‐arginine, NADPH calcium. Salt (200 mM NaCl) caused an...
ABSTRACT Low temperature is an environmental stress that affects crop production and quality regulates the expression of many genes, level a number proteins metabolites. Using leaves from pepper ( Capsicum annum L.) plants exposed to low (8 °C) for different time periods (1 3 d), several key components metabolism reactive nitrogen oxygen species (RNS ROS, respectively) were analysed. After 24 h exposure at 8 °C, exhibited visible symptoms characterized by flaccidity stems leaves. This was...
Post-translational modifications (PTMs) mediated by nitric oxide (NO)-derived molecules have become a new area of research, as they can modulate the function target proteins. Proteomic data shown that ascorbate peroxidase (APX) is one potential targets PTMs NO-derived molecules. Using recombinant pea cytosolic APX, impact peroxynitrite (ONOO–) and S-nitrosoglutathione (GSNO), which are known to mediate protein nitration S-nitrosylation processes, respectively, was analysed. While inhibits...
Nitric oxide (·NO) is a key signaling molecule in different physiological processes of animals and plants. However, little known about the metabolism endogenous ·NO other reactive nitrogen species (RNS) plants under abiotic stress conditions. Using pea exposed to six conditions (high light intensity, low high temperature, continuous light, dark mechanical wounding), several components RNS including content ·NO, S-nitrosothiols (RSNOs) nitrite plus nitrate, enzyme activities...