A5029658726- Inflammasome and immune disorders
- Fibromyalgia and Chronic Fatigue Syndrome Research
- Tryptophan and brain disorders
- Autophagy in Disease and Therapy
- Coenzyme Q10 studies and effects
- Mitochondrial Function and Pathology
- Endoplasmic Reticulum Stress and Disease
- Cardiovascular, Neuropeptides, and Oxidative Stress Research
- Stress Responses and Cortisol
- Metabolism, Diabetes, and Cancer
- Musculoskeletal pain and rehabilitation
- Lysosomal Storage Disorders Research
- Adipose Tissue and Metabolism
- Biochemical Acid Research Studies
- Calcium signaling and nucleotide metabolism
- Dietary Effects on Health
- Cell death mechanisms and regulation
- Phagocytosis and Immune Regulation
- Endometriosis Research and Treatment
- Nuclear Structure and Function
- Cardiac Health and Mental Health
- Biochemical effects in animals
- Cannabis and Cannabinoid Research
- Advanced battery technologies research
- Muscle Physiology and Disorders
Universidad de Sevilla
2013-2024
Centro Andaluz de Biología del Desarrollo
2014-2017
Universidad Pablo de Olavide
2014-2017
Instituto de Salud Carlos III
2014-2017
Centre for Biomedical Network Research on Rare Diseases
2014-2017
Junta de Andalucía
2014-2017
Centro de Investigación Biomédica en Red
2014-2016
Consejo Superior de Investigaciones Científicas
2013-2016
Instituto de Biomedicina de Sevilla
2015
In 2008, we published the first set of guidelines for standardizing research in autophagy. Since then, this topic has received increasing attention, and many scientists have entered field. Our knowledge base relevant new technologies also been expanding. Thus, it is important to formulate on a regular basis updated monitoring autophagy different organisms. Despite numerous reviews, there continues be confusion regarding acceptable methods evaluate autophagy, especially multicellular...
Abstract While NLRP3‐inflammasome has been implicated in cardiovascular diseases, its role physiological cardiac aging is largely unknown. During aging, many alterations occur the organism, which are associated with progressive impairment of metabolic pathways related to insulin resistance, autophagy dysfunction, and inflammation. Here, we investigated molecular mechanisms through NLRP3 inhibition may attenuate aging. Ablation protected mice from age‐related increased sensitivity, reduced...
Inflammation is a hallmark of aging and negatively affecting female fertility. In this study, we evaluate the role NLRP3 inflammasome in ovarian Age-dependent increased expression ovary was observed WT mice during reproductive aging. High NLRP3, caspase-1, IL-1β also granulosa cells from patients with insufficiency. Ablation improved survival pregnancy rates anti-Müllerian hormone levels autophagy ovaries. Deficiency reduced serum FSH estradiol levels. Consistent these results,...
Abstract Gaucher disease (GD) is caused by mutations in the GBA1 gene, which encodes lysosomal β-glucocerebrosidase. Homozygosity for L444P mutation associated with high risk of neurological manifestations are not improved enzyme replacement therapy. Alternatively, pharmacological chaperones (PCs) capable restoring correct folding and trafficking mutant represent promising alternative therapies.Here, we report on how affects mitochondrial function primary fibroblast derived from GD patients....
Impairment in adenosine monophosphate-activated protein kinase (AMPK) activity and NOD-like receptor family, pyrin domain containing 3 (NLRP3) inflammasome activation are associated with several metabolic inflammatory diseases. In this study, we investigated the role of AMPK/NLRP3 axis molecular mechanism underlying pain perception.Impairment AMPK induced by compound C or sunitinib, two inhibitors, provoked hyperalgesia mice (p<0.001) marked NLRP3 increased serum levels interleukin-1β...
Fibromyalgia (FM) is a chronic pain syndrome with unknown etiology and wide spectrum of symptoms such as allodynia, debilitating fatigue, joint stiffness migraine. Recent studies have shown some evidences demonstrating that oxidative stress associated to clinical in FM fibromyalgia. We examined bioenergetic status blood mononuclear cells (BMCs) its association headache patients. The effects oral coenzyme Q(10) (CoQ(10)) supplementation on biochemical markers improvement were also...
Aims: Fibromyalgia (FM) is a prevalent chronic pain syndrome characterized by generalized hyperalgesia associated with wide spectrum of symptoms such as fatigue and joint stiffness. Diagnosis FM difficult due to the lack reliable diagnostic biomarkers, while treatment largely inadequate. We have investigated role coenzyme Q10 (CoQ10) deficiency mitochondrial dysfunction in inflammasome activation blood cells from patients, vitro vivo CoQ10 models. Results: Mitochondrial was accompanied...
Fibromyalgia (FM) is a complex disorder that affects up to 5% of the general population worldwide. Its pathophysiological mechanisms are difficult identify and current drug therapies demonstrate limited effectiveness. Both mitochondrial dysfunction coenzyme Q10 (CoQ10) deficiency have been implicated in FM pathophysiology. We investigated effect CoQ10 supplementation. carried out randomized, double-blind, placebo-controlled trial evaluate clinical gene expression effects forty days...
The NLRP3-inflammasome complex has emerged as an important component of inflammatory processes in metabolic dysfunction induced by high-caloric diets. In this study, we investigate the molecular mechanisms which NLRP3 inhibition may attenuate diet-induced cardiac injury. Here show damage high sugar diet (HSD), fat (HFD) or sugar/fat (HSFD) over 15 weeks. Genetic ablation protected against autophagy induction and apoptotic control. Furthermore, selective small molecule MCC950 resulted similar...
BACKGROUND: The Andean blackberry (Rubus glaucus Benth) is one of Ecuador’s most iconic berries for which a high anthocyanin content has been described. OBJECTIVE: aim the present study was to determine chemical composition and anti-inflammatory potential from Ecuador, with an emphasis on its effects NLRP3 inflammasome activation autophagy processes. RESULTS: extracts were rich in hydroxycinnamates (coumaric acid derivates), addition quercetin kaempferol as principal flavonols. Cyanidin...
Abstract The NLRP3 inflammasome has emerged as an important regulator of metabolic disorders and age-related diseases in NLRP3-deficient mice. In this article, we determine whether, old mice C57BL6J, the inhibitor MCC950 is able to attenuate syndrome providing health benefits. We report that attenuates hepatic dysfunction aged addition, inhibited Pi3K/AKT/mTOR pathway, enhanced autophagy, activated peroxisome proliferator-activated receptor-α vivo vitro. data suggest mediates protective...
We examined lipid peroxidation (LPO) in blood mononuclear cells (BMCs) and plasma, as a marker of oxidative damage, its association to clinical symptoms Fibromyalgia (FM) patients.We conducted case-control correlational study comparing 65 patients 45 healthy controls. Clinical parameters were evaluated using the Impact Questionnaire (FIQ), visual analogues scales (VAS), Beck Depression Inventory (BDI). Oxidative stress was determined by measuring LPO BMCs plasma.We found increased levels...