A5016776458- Neuroscience and Neuropharmacology Research
- Wnt/β-catenin signaling in development and cancer
- Ion channel regulation and function
- Axon Guidance and Neuronal Signaling
- Neuroscience and Neural Engineering
- Hippo pathway signaling and YAP/TAZ
- Genetics and Neurodevelopmental Disorders
- Nerve injury and regeneration
- Neurogenesis and neuroplasticity mechanisms
- Neuroinflammation and Neurodegeneration Mechanisms
- Advanced Fluorescence Microscopy Techniques
- Nicotinic Acetylcholine Receptors Study
- Autophagy in Disease and Therapy
- Digital Holography and Microscopy
- Cancer-related gene regulation
- Cardiac electrophysiology and arrhythmias
- Cell Image Analysis Techniques
- Memory and Neural Mechanisms
- Complement system in diseases
- Alzheimer's disease research and treatments
- Autism Spectrum Disorder Research
- Hemoglobinopathies and Related Disorders
- Congenital heart defects research
- Bone Metabolism and Diseases
- Mitochondrial Function and Pathology
University of Nebraska Medical Center
2012-2023
Nebraska Medical Center
2012-2023
Howard University
2020-2021
Meyer (China)
2015-2017
Institute of Developmental Physiology
2012-2015
University of Nebraska at Omaha
2015
University of California, San Francisco
2008-2014
University of Iowa
1998-2007
Howard Hughes Medical Institute
1998-2007
University of Wisconsin–Madison
2001
In 2008, we published the first set of guidelines for standardizing research in autophagy. Since then, this topic has received increasing attention, and many scientists have entered field. Our knowledge base relevant new technologies also been expanding. Thus, it is important to formulate on a regular basis updated monitoring autophagy different organisms. Despite numerous reviews, there continues be confusion regarding acceptable methods evaluate autophagy, especially multicellular...
Our findings suggest that morphine dysregulates synaptic balance in the hippocampus, a key center for learning and memory, via novel signaling pathway involving reactive oxygen species (ROS), endoplasmic reticulum (ER) stress, autophagy. We demonstrate this study exposure of to hippocampal neurons leads reduction excitatory synapse densities with concomitant enhancement inhibitory activation μ opioid receptor. Furthermore, these effects are mediated by up-regulation intracellular ROS from...
Dendrites are key integrators of synaptic information in neurons and play vital roles neuronal plasticity. Hence, it is necessary that dendrite arborization precisely controlled coordinated with activity to ensure appropriate functional neural network integrity. In the past several years, has become increasingly clear cell intrinsic extrinsic mechanisms contribute dendritic arborization. this review, we will discuss some molecular regulate morphogenesis, particularly cortical hippocampal...
The maintenance of spine and synapse number during development is critical for neuronal circuit formation function. Here we show that delta-catenin, a component the cadherin-catenin cell adhesion complex, regulates morphogenesis development. Genetic ablation or acute knockdown delta-catenin leads to increases in density, accompanied by decrease tetrodotoxin induced plasticity. Our results indicate may mediate conversion activity-dependent signals morphological functional role regulating...
Abstract We present a new approach for retrieving halo-free phase contrast microscopy (hfPC) images by upgrading the conventional PC microscope with an external interferometric module, which generates sufficient data reversing halo artifact. Acquiring four independent intensity images, our first measures haloed maps of sample. solve sample transmission function using physical model image formation under partial spatial coherence. Using this transmission, we can numerically generate...
The ability of neurons to maintain spine architecture and modulate it in response synaptic activity is a crucial component the cellular machinery that underlies information storage pyramidal hippocampus. Here we show critical role for δ-catenin, cadherin-catenin cell adhesion complex, regulating head width length loss Ctnnd2, gene encoding has been associated with intellectual disability observed cri du chat syndrome, suggesting functional roles δ-catenin are vital neuronal integrity higher...
δ-catenin is expressed in excitatory synapses and functions as an anchor for the glutamatergic AMPA receptor (AMPAR) GluA2 subunit postsynaptic density. The glycine 34 to serine (G34S) mutation gene has been found autism spectrum disorder (ASD) patients results loss of at synapses, which presumed underlie ASD pathogenesis humans. However, how G34S causes induce remains unclear. Here, using neuroblastoma cells, we identify that increases glycogen synthase kinase 3β (GSK3β)-dependent...
The LAP [leucine-rich and postsynaptic density-95/Discs large/zona occludens-1 (PDZ)] protein erbin δ-catenin, a component of the cadherin–catenin cell adhesion complex, are highly expressed in neurons associate through PDZ-mediated interaction, but have incompletely characterized neuronal functions. We show that short hairpin RNA-mediated knockdown or genetic ablation δ-catenin severely impaired dendritic morphogenesis hippocampal neurons. Simultaneous loss does not enhance severity this...
In neurons, dendrites form the major sites of information receipt and integration. It is thus vital that, during development, dendritic arbor adequately formed to enable proper neural circuit formation function. While several known processes shape arbor, little those that govern dendrite branching versus extension. Here, we report a new mechanism instructing branch extend. it, glutamate signaling activates mGluR5 receptors promote Ckd5-mediated phosphorylation C-terminal PDZ-binding motif...
Voltage-gated calcium channels mediate excitationcontraction coupling in the skeletal muscle. Their molecular composition, similar to neuronal channels, includes pore-forming α<sub>1</sub> and auxiliary α<sub>2</sub>δ, β, γ subunits. The subunits are least characterized, their subunit interactions unclear. physiological importance of is emphasized by epileptic stargazer mice that lack γ<sub>2</sub>. In this study, we examined basis interaction between γ<sub>1</sub> channel. Our data show...
To investigate the molecular basis of voltage sensor that triggers excitation–contraction (EC) coupling, four-domain pore subunit dihydropyridine receptor (DHPR) was cut in cytoplasmic linker between domains II and III. cDNAs for I-II domain (α1S 1–670) III-IV 701-1873) were expressed dysgenic α1S-null myotubes. Coexpression two fragments resulted complete recovery DHPR intramembrane charge movement voltage-evoked Ca 2+ transients. When separately, EC coupling not recovered. However,...
In order to fully understand brain connectivity and elucidate the mechanisms involved in central nervous system disease, field of neuroscience depends on quantitative studies neuronal structure function. Cell morphology neurite (axonal dendritic) arborization are typically studied by immunohistochemical fluorescence techniques. However, dry mass content intracellular transport rates have largely been under-investigated given inherent difficulties their measurement. Here, spatial light...
Optimal growth as well branching of axons and dendrites is critical for the nervous system function. Neuritic length, arborization, rate determine innervation properties neurons define each cell's computational capability. Thus, to investigate function, we need develop methods instrumentation techniques capable quantifying various aspects neural network formation: neuron process extension, retraction, stability, branching. During last three decades, fluorescence microscopy has yielded...
Alzheimer's disease (AD) is a neurodegenerative syndrome classically depicted by the parenchymal accumulation of extracellular amyloid beta plaques. However, recent findings suggest intraneuronal (iAβ1–42) precedes deposition. Furthermore, pathologic increase in iAβ1–42 has been implicated dysregulation cellular mechanisms critically important axonal transport. Owing to neuronal cell polarity, retrograde and anterograde transport are essential trafficking mechanism necessary convey membrane...
Neuronal voltage-dependent Ca 2+ channels have been isolated previously and shown to contain a primary α 1 pore-forming subunit as well auxiliary 2 δ β subunits, in addition an uncharacterized 95 kDa protein. In the present study, using multiple approaches, we extensively characterized molecular structure of Separation P/Q- N-type neuronal showed that protein is associated exclusively with ω-Conotoxin MVIIC receptor P/Q-type channels. Analysis purified synaptic plasma membranes channels, 1A...