ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTLaser-Flash Kinetic Analysis of the Fast Electron Transfer from Plastocyanin and Cytochrome c6 to Photosystem I. Experimental Evidence on Evolution Reaction MechanismManuel Hervas, Jose A. Navarro, Antonio Diaz, Herve Bottin, Miguel De la RosaCite this: Biochemistry 1995, 34, 36, 11321–11326Publication Date (Print):September 12, 1995Publication History Published online1 May 2002Published inissue 12 September...

Since the first description of apoptosis four decades ago, great efforts have been made to elucidate, both in vivo and vitro, molecular mechanisms involved its regulation. Although role cytochrome c during is well established, relatively little known about participation signaling pathways due essential respiration. To obtain a better understanding onset apoptosis, we used proteomic approach based on affinity chromatography with as bait this study. In approach, novel interaction partners were...

Programmed cell death is an event displayed by many different organisms along the evolutionary scale. In plants, programmed necessary for development and hypersensitive response to stress or pathogenic infection. A common feature in across translocation of cytochrome c from mitochondria cytosol. To better understand role onset a proteomic approach was developed based on affinity chromatography using Arabidopsis thaliana as bait. Using this approach, ten putative new partners were identified....

Iron limitation is the major factor controlling phytoplankton growth in vast regions of contemporary oceans. In this study, a combination thermoluminescence, chlorophyll fluorescence and P700 absorbance measurements have been used to elucidate effects iron deficiency photosynthetic electron transport marine diatom Phaeodactylum tricornutum. Thermoluminescence was determine on Photosystem II activity. Excitation iron-replete tricornutum cells with single turn-over flashes induced appearance...

The redox and acid/base states midpoint potentials of cytochrome b ‐559 have been determined in oxygen‐evolving photosystem II (PS II) particles at room temperature the pH range from 6.5 to 8.5. At 7.5 fresh PS present about 2/3 their its reduced protonated (non‐auto‐oxidizable) high‐potential form 1/3 oxidized non‐protonated low‐potential form. Potentiometric reductive titration shows that couple is pH‐independent ( E 0 , + 380 mV), whereas above 7.6 > 7.6, 140 but becomes pH‐dependent...

Question: Do solid-walled polyethylene tubes and mesh fabric improve the short-term survival of eight Mediterranean tree shrub species often used in restoration arid environments? Location: We conducted two experimental plantations degraded field sites province Almería (SE Spain), under conditions. Methods: One-year-old seedlings Ceratonia siliqua, Juniperus phoenicea, Olea europaea, Pinus halepensis, pinaster, Quercus coccifera, ilex Tetraclinis articulata were planted either sheltered by...

Abstract Hydrogen sulfide regulates essential plant processes, including adaptation responses to stress situations, and the best characterized mechanism of action consists post-translational modification persulfidation. In this study, we reveal first persulfidation proteome described in rice 3443 different persulfidated proteins that participate a broad range biological processes metabolic pathways. addition, comparative proteomics revealed specific involved signaling during drought...

Laser‐flash kinetic absorption spectroscopy has been used to compare the rate constants for electron transfer from reduced plastocyanin and cytochrome c 552 , obtained green alga Monoraphidium braunii photooxidized P700 (P700 + ) in photosystem I (PSI) particles spinach. Sigmoidal protein concentration dependence observed electron‐transfer are both proteins. In absence of added salts, reduction increases as pH decreases approximately 8 5.5, then pH, 3.5, this effect being more pronounced...

The cyanobacterium Synechocystis sp. PCC 6803 possesses an arsenic resistance operon that encodes, among others, ArsH protein. is a flavin mononucleotide (FMN)-containing protein of unknown function and member the family NADPH-dependent FMN reductases. nature its final electron acceptor role in to remained be clarified. Here we have expressed purified conducted intensive biochemical study. We present kinetic evidence supporting quinone reductase activity for ArsH, with preference quinones...

The aim of this study was to investigate the combined effects temperature and light on photosynthetic parameters lipid accumulation in diatom Phaeodactylum tricornutum, a model organism widely used for studies physiology, ecology, biotechnology. Our results highlight importance interaction between intensity influencing growth rates, pigments active photosystems content, efficiency, production fatty acid composition P. tricornutum. Measurements maximum electron transport rate (rETRmax) rETR...

A comparative thermodynamic analysis of photosystem I (PSI) reduction by plastocyanin (Pc) and cytochrome c6 (Cyt) has been carried out laser-flash absorption spectroscopy in the cyanobacteria Anabaena PCC 7119 Synechocystis 6803 as well spinach. These three organisms have reported to exhibit different reaction mechanisms [Hervás, M., Navarro, J. A., Díaz, Bottin, H., & De la Rosa, M. A. (1995) Biochemistry, 34, 11321−11326]. Whereas activation free energy for overall is mainly enthalpic...

The effect of tyrosine nitration on the physicochemical properties and reactivity human respiratory cytochrome c has been extensively analyzed. A set mutants, each bearing only one out five present in wild-type molecule, constructed order to study whole protein. Replacement tyrosines by phenylalanines does not promote significant changes cytochrome. Nitration promotes a drastic decrease (ca. 350 mV) midpoint redox potential, probably induced both 48 67. also intrinsic all mutant proteins....

Under nitroxidative stress, a minor fraction of cytochrome c can be modified by tyrosine nitration. Here we analyze the specific effect nitration tyrosines 46 and 48 on dual role in cell survival death. Our findings reveal that these two solvent‐exposed residues has negligible rate electron transfer from to oxidase, but impairs ability heme protein activate caspase‐9 assembling non‐functional apoptosome. It seems under cellular stress counteracts apoptosis light small amount protein. We...

In addition to the standard NADPH thioredoxin reductases (NTRs), plants hold a plastidic NTR (NTRC), with module fused at C‐terminus. NTRC is an efficient reductant of 2‐Cys peroxiredoxins (2‐Cys Prxs). The interaction and chloroplastic x Prxs has been confirmed in vivo, by bimolecular fluorescence complementation (BiFC) assays, vitro, isothermal titration calorimetry (ITC) experiments. comparison , interacts Prx higher affinity, both domains contributing significantly this interaction, as...