A5085058459- Photosynthetic Processes and Mechanisms
- Algal biology and biofuel production
- Autophagy in Disease and Therapy
- Fungal and yeast genetics research
- Lipid metabolism and biosynthesis
- Plant responses to water stress
- Neural Networks and Applications
- Endoplasmic Reticulum Stress and Disease
- PI3K/AKT/mTOR signaling in cancer
- Neurobiology and Insect Physiology Research
- Plant nutrient uptake and metabolism
- Polyamine Metabolism and Applications
- Nitrogen and Sulfur Effects on Brassica
- Plant Molecular Biology Research
- Cassava research and cyanide
- Fungal Plant Pathogen Control
- Phytoplasmas and Hemiptera pathogens
- Photoreceptor and optogenetics research
- Hydrological Forecasting Using AI
- Evolutionary Algorithms and Applications
- CRISPR and Genetic Engineering
- Fuzzy Logic and Control Systems
- Data Management and Algorithms
- Sulfur Compounds in Biology
- Studies on Chitinases and Chitosanases
Instituto de Bioquímica Vegetal y Fotosíntesis
2015-2025
Universidad de Sevilla
2015-2025
Consejo Superior de Investigaciones Científicas
2013-2022
Institut de Biologie Physico-Chimique
2012
Laboratoire de Biologie Moléculaire et Cellulaire des Eucaryotes
2012
Centre National de la Recherche Scientifique
2012
Sorbonne Université
2012
University of Basel
2001-2011
Fundación PROINPA
2010
Universidad de Cantabria
1993-2009
Microalgae are regarded as promising organisms to develop innovative concepts based on their photosynthetic capacity that offers more sustainable production than heterotrophic hosts. However, realize potential green cell factories, a major challenge is make microalgae easier engineer. A approach for rapid and predictable genetic manipulation use standardized synthetic biology tools workflows. To this end we have developed Modular Cloning toolkit the microalga Chlamydomonas reinhardtii. It...
The essential, rapamycin-sensitive TOR kinases regulate a diverse set of cell growth-related readouts in response to nutrients. Thus, the yeast proteins function as nutrient sensors, particular sensors nitrogen and possibly carbon. However, metabolite(s) that acts upstream is unknown. We investigated role glutamine, preferred source key intermediate metabolism, possible regulator TOR. show glutamine synthetase inhibitor l -methionine sulfoximine (MSX) specifically provokes depletion cells....
Autophagy is a catabolic membrane-trafficking process whereby cells recycle cytosolic proteins and organelles under stress conditions or during development. This degradative mediated by autophagy-related (ATG) that have been described in yeast, animals, more recently plants. In this study, we report the molecular characterization of autophagy unicellular green alga Chlamydomonas reinhardtii. We demonstrate ATG8 protein from (CrATG8) functionally conserved may be used as marker. Like yeast...
In Arabidopsis thaliana, DES1 is the only identified l-Cysteine desulfhydrase located in cytosol, and it involved degradation of cysteine concomitant production H2S this cell compartment. Detailed characterization T-DNA insertion mutants des1 -1 des1-2 has provided insight into role sulfide metabolically generated cytosol as a signaling molecule. Mutations L-CYS DESULFHYDRASE 1 (DES1) impede generation strongly affect plant metabolism. Senescence-associated vacuoles are detected mesophyll...
Abstract Plastid protein homeostasis is critical during chloroplast biogenesis and responses to changes in environmental conditions. Proteases molecular chaperones involved plastid quality control are encoded by the nucleus except for catalytic subunit of ClpP, an evolutionarily conserved serine protease. Unlike its Escherichia coli ortholog, this protease essential cell viability. To study function, we used a recently developed system repressible gene expression alga Chlamydomonas...
The vesicle-inducing protein in plastids (VIPP1) was suggested to play a role thylakoid membrane formation via vesicles. As this functional assignment is under debate, we investigated the function of VIPP1 Chlamydomonas reinhardtii. Using immunofluorescence, localized distinct spots within chloroplast. In VIPP1-RNA interference/artificial microRNA cells, consistently observed aberrant, prolamellar body-like structures at origin multiple layers, which appear coincide with immunofluorescent...
Autophagy is a membrane-trafficking process whereby double-membrane vesicles called autophagosomes engulf and deliver intracellular material to the vacuole for degradation. Atg4 cysteine protease with an essential function in autophagosome formation. Mounting evidence suggests that reactive oxygen species may play role control of autophagy could regulate activity but precise mechanisms remain unclear. In this study, we showed activate model yeast Saccharomyces cerevisiae unraveled molecular...
Autophagy is an intracellular catabolic process that allows cells to recycle unneeded or damaged material maintain cellular homeostasis. This highly dynamic characterized by the formation of double-membrane vesicles called autophagosomes, which engulf and deliver cargo vacuole. Flow through autophagy pathway its degradation in vacuole known as autophagic flux, reflects activity. A number assays have been developed determine flux yeasts, mammals, plants, but it has not examined yet algae....
Hydrogen sulfide is a signaling molecule that regulates essential processes in plants, such as autophagy. In Arabidopsis (Arabidopsis thaliana), hydrogen negatively autophagy independently of reactive oxygen species via an unknown mechanism. Comparative and quantitative proteomic analysis was used to detect abscisic acid-triggered persulfidation reveals main role the control mediated by autophagy-related (ATG) Cys protease AtATG4a. This undergoes specific Cys170 part characteristic catalytic...
Tudor staphylococcal nuclease (TSN; also known as Tudor-SN, p100, or SND1) is a multifunctional, evolutionarily conserved regulator of gene expression, exhibiting cytoprotective activity in animals and plants oncogenic mammals. During stress, TSN stably associates with stress granules (SGs), poorly understood process. Here, we show that the model plant Arabidopsis thaliana, an intrinsically disordered protein (IDP) acting scaffold for large pool other IDPs, enriched granule components well...
Abstract The macrolide rapamycin specifically binds the 12-kD FK506-binding protein (FKBP12), and this complex potently inhibits target of (TOR) kinase. identification TOR in Arabidopsis (Arabidopsis thaliana) revealed that is conserved photosynthetic eukaryotes. However, research on signaling plants has been hampered by natural resistance to rapamycin. Here, we report inactivation treatment a organism. We identified characterized FKBP12 homologs unicellular green alga Chlamydomonas...
Target of rapamycin (TOR) kinases control cell growth through two functionally distinct multiprotein complexes. TOR complex 1 (TORC1) controls temporal and is sensitive to rapamycin, whereas 2 (TORC2) resistant regulates spatial growth. Here, we identified orthologues, TbTOR1 TbTOR2, in the protozoan parasite Trypanosoma brucei, as well orthologues well-known TORC1 TORC2 partners, KOG1/raptor AVO3/rictor. TbTOR proteins differ their functions, subcellular localization, sensitivity. by...
All aerobic organisms have developed sophisticated mechanisms to prevent, detect and respond cell damage caused by the unavoidable production of reactive oxygen species (ROS). Plants algae are able synthesize specific pigments in chloroplast called carotenoids prevent photo-oxidative highly by-products photosynthesis. In this study we used unicellular green alga Chlamydomonas reinhardtii demonstrate that defects carotenoid biosynthesis lead activation autophagy, a membrane-trafficking...
The accumulation of unfolded/misfolded proteins in the endoplasmic reticulum (ER) results activation stress responses, such as unfolded protein response or catabolic process autophagy to ultimately recover cellular homeostasis. ER also promotes production reactive oxygen species, which play an important role regulation. However, it remains unknown whether species are involved stress-induced autophagy. In this study, we provide evidence connecting redox imbalance caused by and model...
Microspores are reprogrammed towards embryogenesis by stress. Many microspores die after this stress, limiting the efficiency of microspore embryogenesis. Autophagy is a degradation pathway that plays critical roles in stress response and cell death. In animals, cathepsins have an integral role autophagy degrading autophagic material; less known plants. Plant papain-like C1A cysteine proteases involved many physiological processes, including programmed We analysed involvement death, relation...
Autophagy is a major catabolic pathway by which eukaryotic cells deliver unnecessary or damaged cytoplasmic material to the vacuole for its degradation and recycling in order maintain cellular homeostasis. Control of autophagy has been associated with production reactive oxygen species several organisms, including plants algae, but precise regulatory molecular mechanisms remain unclear. Here, we show that ATG4 protease, an essential protein autophagosome biogenesis, plays central role redox...
Target of rapamycin complex 1 (TORC1) is a central regulator cell growth. It balances anabolic and catabolic processes in response to nutrients, growth factors, energy availability. Nitrogen- carbon-containing metabolites have been shown activate TORC1 yeast, animals, plants. Here, we show that phosphorus (P) regulates signaling the model green alga Chlamydomonas (Chlamydomonas reinhardtii) via LST8, conserved subunit interacts with kinase domain TOR. P starvation results sharp decrease LST8...
Significance Photosynthetic organisms are responsible for the incorporation of inorganic carbon in biosphere through a fundamental process known as fixation. This reaction allows reduction carbon, mostly atmospheric CO 2 , to organic compounds such carbohydrates and amino acids. Despite biological relevance fixation nature, how photosynthetic cells sense availability remains poorly understood. Using model microalga Chlamydomonas reinhardtii we found that assimilation regulates activity...
Reversible protein phosphorylation plays an important role in many cellular processes. However, a simple and reliable method to measure changes the extent of is lacking. Here, we present quantitate occurring response stimulus. The consists three steps: ( i ) enzymatic digestion H 2 16 O or isotopically enriched 18 label individual pools differentially phosphorylated proteins; ii affinity selection phosphopeptides from combined digests by immobilized metal-affinity chromatography; iii...
Hydrogen sulfide is already recognized as an important signaling molecule in mammalian systems, and emerging data suggest that H2S a just nitric oxide (NO) H2O2 plants. Although generated chloroplasts mitochondria, it present predominantly the charged HS- form due to basic pH inside both organelles, thus requiring active transporter, which yet be identified, released. In Arabidopsis, we found cytosolic L-cysteine desulfhydrase DES1 involved degradation of cysteine, therefore responsible for...