A5063607135- Fermentation and Sensory Analysis
- Fungal and yeast genetics research
- Cell death mechanisms and regulation
- Endoplasmic Reticulum Stress and Disease
- Mitochondrial Function and Pathology
- Horticultural and Viticultural Research
- Infant Nutrition and Health
- Autophagy in Disease and Therapy
- Microbial Metabolic Engineering and Bioproduction
- Sphingolipid Metabolism and Signaling
- ATP Synthase and ATPases Research
- DNA Repair Mechanisms
- Lipid Membrane Structure and Behavior
- Plant-Microbe Interactions and Immunity
- Pediatric Hepatobiliary Diseases and Treatments
- Photosynthetic Processes and Mechanisms
- Food Quality and Safety Studies
- Biofuel production and bioconversion
- Cellular transport and secretion
- Fungal Biology and Applications
- Signaling Pathways in Disease
- Genetics, Aging, and Longevity in Model Organisms
- RNA Interference and Gene Delivery
- Peptidase Inhibition and Analysis
- Enzyme Catalysis and Immobilization
University of Minho
2015-2024
Clinical Academic Center of Braga
2016
In-Q-Tel
2016
Instituto Gulbenkian de Ciência
1990
Recent evidence has revealed the occurrence of an apoptotic phenotype in Saccharomyces cerevisiae that is inducible with oxidative stress. Here, exposure S. to 20–200 mM acetic acid for 200 min at pH 3·0 resulted cell death. Yeast mortality induced by 120–200 was not inhibited cycloheximide and accompanied ultrastructural alterations typical necrosis. In contrast, associated death 20–80 included: (i) cycloheximide-inhibitable chromatin condensation along nuclear envelope; (ii)...
Apoptosis-inducing factor (AIF), a key regulator of cell death, is essential for normal mammalian development and participates in pathological apoptosis. The proapoptotic nature AIF its mode action are controversial. Here, we show that the yeast homologue Ynr074cp controls Similar to AIF, located mitochondria translocates nucleus cells response apoptotic stimuli. Purified degrades nuclei plasmid DNA. YNR074C disruption rescues from oxygen stress delays age-induced Conversely, overexpression...
Evidence is presented that mitochondria are implicated in the previously described programmed cell death (PCD) process induced by acetic acid Saccharomyces cerevisiae. In yeast cells undergoing a PCD acid, translocation of cytochrome c (CytC) to cytosol and reactive oxygen species production, two events known be proapoptotic mammals, were observed. Associated with these events, reduction consumption mitochondrial membrane potential was found. Enzymatic assays showed activity complexbc 1...
During the last years, several reports described an apoptosis-like programmed cell death process in yeast response to different environmental aggressions. Here, evidence is presented that hyperosmotic stress caused by high glucose or sorbitol concentrations culture medium induces Saccharomyces cerevisiae a accompanied morphological and biochemical indicators of apoptotic death, namely chromatin condensation along nuclear envelope, mitochondrial swelling reduction cristae number, production...
Colorectal carcinoma (CRC) is one of the most common causes cancer-related mortality. Short-chain fatty acids secreted by dietary propionibacteria from intestine, such as acetate, induce apoptosis in CRC cells and may therefore be relevant prevention therapy. We previously reported that acetic acid-induced Saccharomyces cerevisiae involves partial vacuole permeabilization release Pep4p, yeast cathepsin D (CatD), which has a protective role this process. In cancer cells, lysosomes have...
In yeast the use of rhodamine 123 (Rh123) has been restricted to evaluation mitochondrial respiratory function including discrimination between respiratory-competent and -deficient cells. This study describes optimization validation a low-concentration Rh123 staining protocol for flow-cytometric assessment membrane potential (ΔΨm) changes in whole The optimized was validated by compounds that specifically affect energetics. Epifluorescence microscopy used monitor distribution within cell....
Saccharomyces cerevisiae has been reported to die, under certain conditions, from programmed cell death with apoptotic markers. One of the most important markers is chromosomal DNA fragmentation as indicated by terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL) staining. We found TUNEL staining in S. be a consequence both single- and double-strand breaks, whereas situ ligation specifically stained breaks. Cells treated hydrogen peroxide or acetic acid positively for assay...
Adenine nucleotide translocator (ANT) is a mitochondrial inner membrane protein involved in the ADP/ATP exchange and component of permeability transition pore (PTP). In mammalian apoptosis, PTP can mediate outer permeabilization (MOMP), which suspected to be responsible for release apoptogenic factors, including cytochrome c. Although c yeast apoptosis has previously been reported, it not known how occurs. Herein we used genetics investigate whether depletion proteins putatively MOMP affects...
The inositolphosphosphingolipid phospholipase C (Isc1p) of Saccharomyces cerevisiae belongs to the family neutral sphingomyelinases that generates bioactive sphingolipid ceramide. In this work role Isc1p in oxidative stress resistance and chronological lifespan was investigated. Loss resulted a higher sensitivity hydrogen peroxide associated with an increase markers, namely intracellular oxidation, protein carbonylation, lipid peroxidation. Microarray analysis showed deficiency up-regulated...
We have previously shown that acetic acid activates a mitochondria-dependent death process in Saccharomyces cerevisiae and the ADP/ATP carrier (AAC) is required for mitochondrial outer membrane permeabilization cytochrome c release. Mitochondrial fragmentation degradation also been response to this stimulus. Herein, we show autophagy not active cells undergoing acid-induced apoptosis therefore responsible degradation. Furthermore, found vacuolar protease Pep4p AAC proteins role using yeast...
Abstract Acetate is a short-chain fatty acid secreted by Propionibacteria from the human intestine, known to induce mitochondrial apoptotic death in colorectal cancer (CRC) cells. We previously established that acetate also induces lysosome membrane permeabilization CRC cells, associated with release of lysosomal protease cathepsin D (CatD), which has well-established role cascade. Unexpectedly, we showed CatD an antiapoptotic this process, as pepstatin A (a inhibitor) increased...
Acetic acid is mostly known as a toxic by-product of alcoholic fermentation carried out by Saccharomyces cerevisiae, which it frequently impairs. The more recent finding that acetic triggers apoptotic programmed cell death (PCD) in yeast sparked an interest to develop strategies modulate this process, improve several biotechnological applications, but also for biomedical research. Indeed, acetate can trigger apoptosis cancer cells, suggesting its exploitation anticancer compound. Therefore,...
// Sara Alves 1 , Lisandra Castro Maria Sofia Fernandes 3 Rita Francisco Paula Muriel Priault 2 Susana Rodrigues Chaves Mary Pat Moyer 4 Carla Oliveira Raquel Seruca Manuela Côrte-Real João Sousa 1, * Ana Preto CBMA - Centre of Molecular and Environmental Biology, Department University Minho, Campus de Gualtar, 4710-057 Braga, Portugal CNRS, UMR5095, Bordeaux 2, Bordeaux, France IPATIMUP Institute Pathology Immunology the Porto, Instituto Investigação e...
Prostate cancer and osteosarcoma are the second most common type of affecting men fifth malignancy among adolescents, respectively. The use non-toxic natural or natural-derived products has been one current strategies for therapy, owing to reduced risks induced-chemoresistance development absence secondary effects. In this perspective, lactoferrin (Lf), a protein derived from milk, emerges as promising anticancer agent due its well-recognized cytotoxicity anti-metastatic activity. Here, we...
Yeast cells lacking the metacaspase‐encoding gene YCA1 (Δ yca1 ) were compared with wild‐type (WT) respect to occurrence, nature and time course of acetic‐acid triggered death. We show that Δ undergo programmed cell death (PCD) a rate lower than WT PCD in is caused at least part by caspase activity Yca1p. Since this effect lost, but z‐VAD‐fmk does not prevent both death, occurs via Yca1p non‐caspase route similar characteristics.
The yeast Saccharomyces cerevisiae undergoes a mitochondrial-dependent programmed cell death in response to different stimuli, such as acetic acid, with features similar those of mammalian apoptosis. However, the upstream signaling events this process, including leading mitochondrial membrane permeabilization, are still poorly characterized. Changes sphingolipid metabolism have been linked modulation apoptosis both and cells, ceramides detected mitochondria upon apoptotic stimuli. In study,...
Breast cancer is the most common type of affecting women. Despite good prognosis when detected early, significant challenges remain in treatment metastatic breast cancer. The recruitment vacuolar H+-ATPase (V-H+-ATPase) to plasma membrane, where it mediates acidification tumor microenvironment (TME), a recognized feature involved acquisition phenotype Therefore, inhibitors this pump have emerged as promising anticancer drugs. Lactoferrin (Lf) natural pro-apoptotic iron-binding glycoprotein...