A5020021717- Endoplasmic Reticulum Stress and Disease
- Autophagy in Disease and Therapy
- Sphingolipid Metabolism and Signaling
- Cell death mechanisms and regulation
- Mitochondrial Function and Pathology
- Fungal and yeast genetics research
- DNA Repair Mechanisms
- Plant-Microbe Interactions and Immunity
- Horticultural and Viticultural Research
- Fermentation and Sensory Analysis
- Parkinson's Disease Mechanisms and Treatments
- Lipid Membrane Structure and Behavior
- Infant Nutrition and Health
- Toxoplasma gondii Research Studies
- Fungal Plant Pathogen Control
- Cancer-related Molecular Pathways
- RNA Research and Splicing
- Biofuel production and bioconversion
- Signaling Pathways in Disease
- Ubiquitin and proteasome pathways
- Thyroid Cancer Diagnosis and Treatment
- Plant Pathogens and Resistance
- Viral Infectious Diseases and Gene Expression in Insects
- Pesticide Exposure and Toxicity
- Nuclear Structure and Function
University of Minho
2014-2025
Hospital Universitario Fundación Jiménez Díaz
2011
Scripps Research Institute
2008-2010
Howard Hughes Medical Institute
2001
Rockefeller University
2001
Colorectal carcinoma (CRC) is one of the most common causes cancer-related mortality. Short-chain fatty acids secreted by dietary propionibacteria from intestine, such as acetate, induce apoptosis in CRC cells and may therefore be relevant prevention therapy. We previously reported that acetic acid-induced Saccharomyces cerevisiae involves partial vacuole permeabilization release Pep4p, yeast cathepsin D (CatD), which has a protective role this process. In cancer cells, lysosomes have...
Due to increasing demand for high and stable crop production, human populations are highly dependent on pesticide use growing storing food. Environmental monitoring of these agrochemicals is therefore utmost importance, because their collateral effects ecosystem health. Even though most current-use analytical methods achieve low detection limits, they require procedures that too complex costly routine monitoring. As such, there has been an increased interest in biosensors as alternative or...
Cks proteins associate with cyclin-dependent kinases and have therefore been assumed to play a direct role in cell cycle regulation.Mammals two paralogs, Cks1 Cks2, individually deleting the gene encoding either mouse has previously shown not impact viability.In this study we show that simultaneously disrupting CKS1 CKS2 leads embryonic lethality, embryos dying at or before morula stage after only four division cycles.RNA interference (RNAi)-mediated silencing of CKS genes fibroblasts (MEFs)...
We have previously shown that acetic acid activates a mitochondria-dependent death process in Saccharomyces cerevisiae and the ADP/ATP carrier (AAC) is required for mitochondrial outer membrane permeabilization cytochrome c release. Mitochondrial fragmentation degradation also been response to this stimulus. Herein, we show autophagy not active cells undergoing acid-induced apoptosis therefore responsible degradation. Furthermore, found vacuolar protease Pep4p AAC proteins role using yeast...
Abstract Acetate is a short-chain fatty acid secreted by Propionibacteria from the human intestine, known to induce mitochondrial apoptotic death in colorectal cancer (CRC) cells. We previously established that acetate also induces lysosome membrane permeabilization CRC cells, associated with release of lysosomal protease cathepsin D (CatD), which has well-established role cascade. Unexpectedly, we showed CatD an antiapoptotic this process, as pepstatin A (a inhibitor) increased...
Acetic acid is mostly known as a toxic by-product of alcoholic fermentation carried out by Saccharomyces cerevisiae, which it frequently impairs. The more recent finding that acetic triggers apoptotic programmed cell death (PCD) in yeast sparked an interest to develop strategies modulate this process, improve several biotechnological applications, but also for biomedical research. Indeed, acetate can trigger apoptosis cancer cells, suggesting its exploitation anticancer compound. Therefore,...
// Sara Alves 1 , Lisandra Castro Maria Sofia Fernandes 3 Rita Francisco Paula Muriel Priault 2 Susana Rodrigues Chaves Mary Pat Moyer 4 Carla Oliveira Raquel Seruca Manuela Côrte-Real João Sousa 1, * Ana Preto CBMA - Centre of Molecular and Environmental Biology, Department University Minho, Campus de Gualtar, 4710-057 Braga, Portugal CNRS, UMR5095, Bordeaux 2, Bordeaux, France IPATIMUP Institute Pathology Immunology the Porto, Instituto Investigação e...
The yeast Saccharomyces cerevisiae undergoes a mitochondrial-dependent programmed cell death in response to different stimuli, such as acetic acid, with features similar those of mammalian apoptosis. However, the upstream signaling events this process, including leading mitochondrial membrane permeabilization, are still poorly characterized. Changes sphingolipid metabolism have been linked modulation apoptosis both and cells, ceramides detected mitochondria upon apoptotic stimuli. In study,...
Mutations and multiplications in the SNCA gene , encoding alpha-synuclein (aSyn), are associated with familial forms of Parkinson's disease (PD). We report identification a novel missense mutation (NM_000345.4, cDNA 174G>C; protein K58N) PD patient using whole exome sequencing, describe comprehensive molecular cellular analysss effects this mutation. The exhibited typical sporadic early onset benign course. Biophysical studies revealed that K58N substitution causes local structural effects,...
Mitochondrial outer membrane permeabilization is a key event in apoptosis processes leading to the release of lethal factors. We have previously shown that absence ADP/ATP carrier (AAC) proteins (yeast orthologues mammalian ANT proteins) increased resistance yeast cells acetic acid, preventing MOMP and cytochrome c from mitochondria during acid - induced apoptosis. On other hand, deletion POR1 voltage-dependent anion channel VDAC) sensitivity acid. In present work, we aimed further...
Acetic acid triggers apoptotic cell death in Saccharomyces cerevisiae, similar to mammalian apoptosis. To uncover novel regulators of this process, we analyzed whether impairing MAPK signaling affected acetic acid-induced apoptosis and found the mating-pheromone response and, especially, wall integrity pathways were major mediators, especially latter, which characterized further. Screening downstream effectors pathway, namely targets transcription factor Rlm1p, highlighted decreased...
We have previously shown that the yeast Cathepsin D (CatD) Pep4p translocates from vacuole to cytosol during acetic acid‐induced apoptosis and is required for efficient mitochondrial degradation, though its specific role in this process still elusive. Here, we show protective of depends on catalytic activity independent voltage‐dependent anion channel Por1p (which has no degradation) but dependent AAC proteins, adenine nucleotide translocator. Our results demonstrate a differential interplay...
Cks1, Cdk1 (Cdc28), and the proteasome are required for efficient transcriptional induction of GAL1 other genes in Saccharomyces cerevisiae. We show here that one function these proteins is to reduce nucleosome density on chromatin a gene induction-specific manner. The requirement Cks1 can be bypassed if reduced by an alternative pathway, indicating this primary context induction. further Cdk1, 19S subunit recruited binding directly histone H4 amino-terminal tail. However, activity does not...