A5036663290- Genetics, Aging, and Longevity in Model Organisms
- Circadian rhythm and melatonin
- Mitochondrial Function and Pathology
- Birth, Development, and Health
- Autophagy in Disease and Therapy
- Fish biology, ecology, and behavior
- Diet and metabolism studies
- Adipose Tissue and Metabolism
- Endoplasmic Reticulum Stress and Disease
- RNA Research and Splicing
- Hormonal Regulation and Hypertension
- CRISPR and Genetic Engineering
- Spaceflight effects on biology
- Fish Ecology and Management Studies
- RNA modifications and cancer
- Reproductive Biology and Fertility
- Dietary Effects on Health
- MicroRNA in disease regulation
- Telomeres, Telomerase, and Senescence
- Reproductive biology and impacts on aquatic species
- Neuroendocrine regulation and behavior
- Pancreatic function and diabetes
- Frailty in Older Adults
- Physiological and biochemical adaptations
- Aquaculture Nutrition and Growth
University of Cologne
2015-2024
Cologne Excellence Cluster on Cellular Stress Responses in Aging Associated Diseases
2015-2024
Max Planck Institute for Biology of Ageing
2015-2024
The University of Adelaide
2024
National Hospital for Neurology and Neurosurgery
2024
University College London
2006-2024
Evanston Hospital
2022
Baylor College of Medicine
2007-2016
Max Planck Society
2000-2014
The University of Texas Southwestern Medical Center
2009
In 2008, we published the first set of guidelines for standardizing research in autophagy. Since then, this topic has received increasing attention, and many scientists have entered field. Our knowledge base relevant new technologies also been expanding. Thus, it is important to formulate on a regular basis updated monitoring autophagy different organisms. Despite numerous reviews, there continues be confusion regarding acceptable methods evaluate autophagy, especially multicellular...
PMR1, a Ca(2+)-adenosine triphosphatase (ATPase) homologue in the yeast Saccharomyces cerevisiae localizes to novel Golgi-like organelle. Consistent with Golgi localization, bulk of PMR1 comigrates markers subcellular fractionation experiments, and staining by indirect immunofluorescence reveals punctate pattern resembling yeast. However, shows only partial colocalization known markers, KEX2 SEC7, double-label experiments. The effect on function is indicated pleiotropic defects various...
The daf-12 gene acts at the convergence of pathways regulating larval diapause, developmental age, and adult longevity in Caenorhabditis elegans. It encodes a nuclear receptor most closely related to two C. elegans receptors, NHR-8 NHR-48, Drosophila DHR96, vertebrate vitamin D pregnane-X receptors. has three predicted protein isoforms, which contain DNA- ligand-binding domains, one contains domain only. Mutations cluster but correspond distinct phenotypic classes. DAF-12 is expressed widely...
The National Institute on Aging Interventions Testing Program (ITP) evaluates agents hypothesized to increase healthy lifespan in genetically heterogeneous mice. Each compound is tested parallel at three sites, and all results are published. We report the effects of lifelong treatment mice with four not previously tested: Protandim, fish oil, ursodeoxycholic acid (UDCA) metformin – latter without rapamycin, two drugs examined: 17-α-estradiol nordihydroguaiaretic (NDGA), doses greater less...
Abstract Animal lifespan is regulated by conserved metabolic signalling pathways and specific transcription factors, but whether these affect common downstream mechanisms remains largely elusive. Here we show that NCL-1/TRIM2/Brat tumour suppressor extends limits nucleolar size in the major C. elegans longevity pathways, as part of a convergent mechanism focused on nucleolus. Long-lived animals representing distinct exhibit small nucleoli, decreased expression rRNA, ribosomal proteins,...
Abstract Autophagy, an evolutionarily conserved cytoplasmic degradation system, has been implicated as a convergent mechanism in various longevity pathways. Autophagic activity decreases with age several organisms, but the underlying is unclear. Here, we show that expression of Rubicon, negative regulator autophagy, increases aged worm, fly and mouse tissues at transcript and/or protein levels, suggesting age-dependent increase Rubicon impairs autophagy over time, thereby curtails animal...
Small cell size preserves the function of hematopoietic stem cells (HSCs); HSC enlargement during aging causes their dysfunction.
Physiological homeostasis becomes compromised during ageing, as a result of impairment cellular processes, including transcription and RNA splicing1-4. However, the molecular mechanisms leading to loss transcriptional fidelity are so far elusive, ways preventing it. Here we profiled analysed genome-wide, ageing-related changes in processes across different organisms: nematodes, fruitflies, mice, rats humans. The average elongation speed (RNA polymerase II speed) increased with age all five...
ABSTRACT From egg through adult, C. elegans has six life stages including an option for dauer formation and diapause at larval stage L3 in adverse environments. Somatic cells throughout the organism make consistent choices advance unison, suggesting a mechanism of coordinate regulation these transitions. Earlier studies showed that daf-12, which encodes nuclear receptor (W. Yeh, 1991, Doctoral Thesis. University Missouri-Columbia), regulates formation; epistasis experiments placed daf-12...
Broad aspects of Caenorhabditis elegans life history, including larval developmental timing, arrest at the dauer diapause, and longevity, are regulated by nuclear receptor DAF-12. Endogenous DAF-12 ligands 3-keto bile acid-like steroids, called dafachronic acids, which rescue defects hormone-deficient mutants, such as daf-9/cytochrome P450 daf-36/Rieske oxygenase, activate Here we examined effect acid on pathways controlling lifespan. Dafachronic supplementation shortened lifespan long-lived...
In response to the environment, nematode C. elegans must choose between arrest at a long-lived alternate third larval stage, dauer diapause, or reproductive development. This decision may ultimately be mediated by daf-9, cytochrome P450 related steroidogenic hydroxylases and its cognate nuclear receptor daf-12, implying organism-wide coordination lipophilic hormones. Accordingly, here we show that daf-9(+) works cell non-autonomously bypass promote gonadal outgrowth. Among daf-9-expressing...
In response to small-molecule signals such as retinoids or steroids, nuclear receptors activate gene expression regulate development in different tissues. MicroRNAs turn off target within cells by binding complementary regions messenger RNA transcripts, and they have been broadly implicated disease. Here we show that the Caenorhabditis elegans receptor DAF-12 its steroidal ligand directly promoters of let-7 microRNA family members down-regulate hbl-1 , which drives progression epidermal stem...