A5014660760- Muscle metabolism and nutrition
- Adipose Tissue and Metabolism
- Muscle Physiology and Disorders
- Mitochondrial Function and Pathology
- Genetics, Aging, and Longevity in Model Organisms
- High Altitude and Hypoxia
- Exercise and Physiological Responses
- Cardiac Ischemia and Reperfusion
- Diet and metabolism studies
- Nutrition and Health in Aging
- Cardiovascular Effects of Exercise
- Cardiovascular and exercise physiology
- Neuroscience of respiration and sleep
- Coenzyme Q10 studies and effects
- Metabolism, Diabetes, and Cancer
- Heart Rate Variability and Autonomic Control
- Genetics and Physical Performance
- Heat shock proteins research
- Genomics, phytochemicals, and oxidative stress
- Hemoglobin structure and function
- Vitamin C and Antioxidants Research
- Sports Performance and Training
- Cardiac Arrest and Resuscitation
- Cancer, Hypoxia, and Metabolism
- Biochemical Acid Research Studies
Colorado State University
2016-2025
Cedars-Sinai Medical Center
2024
Dr. John T. Macdonald Foundation
2017
University of Miami
2017
University of Florida
1998-2010
University of Colorado Health
2009
University of Colorado Denver
2009
Defense Advanced Research Projects Agency
2009
University of Colorado Hospital
2009
Appalachian State University
2006
The National Institute on Aging Interventions Testing Program (ITP) evaluates agents hypothesized to increase healthy lifespan in genetically heterogeneous mice. Each compound is tested parallel at three sites, and all results are published. We report the effects of lifelong treatment mice with four not previously tested: Protandim, fish oil, ursodeoxycholic acid (UDCA) metformin – latter without rapamycin, two drugs examined: 17-α-estradiol nordihydroguaiaretic (NDGA), doses greater less...
Metformin and exercise independently improve insulin sensitivity decrease the risk of diabetes. was also recently proposed as a potential therapy to slow aging. However, recent evidence indicates that adding metformin antagonizes exercise-induced improvement in cardiorespiratory fitness. The purpose this study test hypothesis diminishes fitness after aerobic training (AET) by inhibiting skeletal muscle mitochondrial respiration protein synthesis older adults (62 ± 1 years). In double-blinded...
Experimental studies examining the effects of regular exercise on cardiac responses to ischemia and reperfusion (I/R) are limited. Therefore, these experiments examined endurance training myocardial biochemical physiological during in vivo I/R. Female Sprague-Dawley rats (4 mo old) were randomly assigned either a sedentary control group or an group. After 10-wk program, animals anesthetized mechanically ventilated, chest was opened by thoracotomy. Coronary occlusion achieved ligature around...
These experiments examined the independent effects of short-term exercise and heat stress on myocardial responses during in vivo ischemia-reperfusion (I/R). Female Sprague-Dawley rats (4 mo old) were randomly assigned to one four experimental groups: 1) control, 2) 3 consecutive days treadmill [60 min/day at 60–70% maximal O 2 uptake (V˙o max )], 3) 5 (60 60–70%V˙o ), 4) whole body (15 min 42°C). Twenty-four hours after or exercise, animals anesthetized mechanically ventilated, chest was...
Improved endurance exercise performance in adult humans after sprint interval training (SIT) has been attributed to mitochondrial biogenesis. However, muscle protein synthesis (MPS) and biogenesis during SIT have not measured, nor sex-specific differences. We hypothesized that males females would similar rates of MPS, biogenesis, individual proteins SIT. Deuterium oxide (D2O) was orally administered 21 adults [11 male, 10 female; mean age, 23±1 yr; body mass index (BMI), 22.8±0.6 kg/m(2);...
Exercise provides protection against myocardial ischemia-reperfusion (IR) injury. Understanding the mechanisms of this may lead to new interventions for prevention and/or treatment heart disease. Although presently these are not well understood, reports suggest that manganese superoxide dismutase (MnSOD) and calpain be critical mediators protection. We hypothesized an exercise-induced increase in MnSOD would provide cardioprotection by attenuating IR-induced oxidative modification...
Summary It is proposed that caloric restriction (CR) increases mitochondrial biogenesis. However, it not clear why CR an energetically costly biosynthetic process. We hypothesized 40% would decrease protein synthesis and be regulated by translational rather than transcriptional mechanisms. assessed cumulative over 6 weeks its regulation in the liver, heart, skeletal muscle of young (6 month), middle (12 old (24 month) male B6D2F1 mice were lifelong or ad lib (AL) controls. Mitochondrial was...
Consuming protein following exercise has been shown to stimulate synthesis acutely in skeletal muscle and recommended prevent sarcopenia. It is not known, however, whether acute stimulation persists long term or includes cell division. We asked here consuming during aerobic training increases long-term DNA rates of adult humans. Sixteen previously untrained participants (50 ± 8 yr) consumed either a carbohydrate drink each session 6 wk treadmill training. A younger group provided...
Abstract Background Successful strategies to halt or reverse sarcopenia require a basic understanding of the factors that cause muscle loss with age. Acute periods in older individuals have an incomplete recovery mass and strength, thus accelerating sarcopenic progression. The purpose current study was further understand mechanisms underlying failure old animals completely recover function after period hindlimb unloading. Methods Hindlimb unloading used induce atrophy Fischer 344–Brown...
Abstract The causes of the decline in skeletal muscle mass and function with age, known as sarcopenia, are poorly understood. Nutrition (calorie restriction) interventions impact many cellular processes increase lifespan preserve age. As we previously observed an life span aging mice on a ketogenic diet (KD), aimed to investigate effect KD maintenance age potential molecular mechanisms this action. Twelve‐month‐old were assigned isocaloric control or until 16 26 months at which time was...
Mice bred in 2017 and entered into the C2017 cohort were tested for possible lifespan benefits of (R/S)-1,3-butanediol (BD), captopril (Capt), leucine (Leu), Nrf2-activating botanical mixture PB125, sulindac, syringaresinol, or combination rapamycin acarbose started at 9 16 months age (RaAc9, RaAc16). In male mice, Rapa Aca led to a longer than either two prior cohorts mice treated with only, suggesting that this drug was more potent its components used alone. females, receiving both drugs...
We examined the effects of 3 days exercise in a cold environment on expression left ventricular (LV) heat shock proteins (HSPs) and contractile performance during vivo ischemia-reperfusion (I/R). Sprague-Dawley rats were divided into following three groups ( n = 12/group): 1) control, 2) (60 min/day) at 4°C (E-Cold), 3) 25°C (E-Warm). Left anterior descending coronary occlusion was maintained for 20 min, followed by 30 min reperfusion. Compared with control group, both E-Cold E-Warm higher P...
Endurance exercise provides cardioprotection against ischemia-reperfusion (I/R) injury. Exercise-induced is associated with increases in cytoprotective proteins, including heat shock protein 72 (HSP72) and antioxidant enzyme activity. On the basis of reported half-life these putative cardioprotective we hypothesized that exercise-induced I/R injury would be lost within days after cessation exercise. To test this, male rats (4 mo) were randomly assigned to one five experimental groups: 1)....
Chronic inhibition of the protein synthesis regulator mTORC1 through rapamycin extends life span in mice, with longer extension females than males. Whether treatment inhibits or whether it does so differently between sexes has not been examined. UM-HET3 mice were fed a control rapamycin-supplemented (Rap) diet for 12 weeks. Protein mixed, cytosolic (cyto), and mitochondrial (mito) fractions DNA signaling determined skeletal muscle, heart, liver. In both sexes, mito was maintained muscle from...
The measurement of mitochondrial biogenesis is important in the determination aging and disease processes assessment countermeasurements to them. We argue that frequently used assessments cell signaling, protein mRNA, expression or enzyme activity, density are not measurements can lead conclusions about biogenesis. Instead, we propose only synthesis indicative Clarification this issue will hopefully result further differentiation for morphological functional changes mitochondria.
Here, we have described and validated a strategy for monitoring skeletal muscle protein synthesis rates in rodents humans over days or weeks from blood samples. We based this approach on label incorporation into proteins that are synthesized specifically escape the circulation. Heavy water labeling combined with sensitive tandem mass spectrometric analysis allowed integrated of tissue across proteome to be measured several weeks. Fractional rate (FSR) plasma creatine kinase M-type (CK-M)...
Control of protein homeostasis is fundamental to the health and longevity all organisms. Because rate synthesis by ribosomes a central control point in this process, regulation, maintenance ribosome function could have amplified importance overall regulatory circuit. Indeed, ribosomal defects are commonly associated with loss homeostasis, aging, disease (1-4), whereas improved implying optimal function, resistance increased lifespan (5-7). To maintain high-quality population within cell,...
Purpose: It is well established that high-load resistance exercise (HLRE) can stimulate myofibrillar accretion. Additionally, recent studies suggest HLRE also mitochondrial biogenesis and respiratory function. However, in several clinical situations, the use of with high loading may not constitute a viable approach. Low-load blood flow restricted (BFRRE) has emerged as time-effective low-load alternative to unknown if BFRRE If so, could provide feasible strategy muscle metabolic health....
Muscle fibre cross sectional area is enhanced with massage in the form of cyclic compressive loading during regrowth after atrophy. Massage enhances protein synthesis myofibrillar and cytosolic, but not mitochondrial fraction, muscle regrowth. Focal adhesion kinase activation satellite cell number are elevated muscles undergoing DNA synthesis, contralateral non-massaged limb. a potential anabolic intervention