A5004309424- Cardiac Ischemia and Reperfusion
- Cardiovascular Function and Risk Factors
- Cardiac Arrest and Resuscitation
- Cardiac electrophysiology and arrhythmias
- Transplantation: Methods and Outcomes
- Anesthesia and Neurotoxicity Research
- Organ Transplantation Techniques and Outcomes
- Adipose Tissue and Metabolism
- Mitochondrial Function and Pathology
- Hyperglycemia and glycemic control in critically ill and hospitalized patients
- Advanced MRI Techniques and Applications
- Advanced NMR Techniques and Applications
- Cardiovascular and exercise physiology
- Cardiac Imaging and Diagnostics
- Cardiovascular Disease and Adiposity
- Mechanical Circulatory Support Devices
- Fuel Cells and Related Materials
- Organ Donation and Transplantation
- Muscle metabolism and nutrition
- Cardiac pacing and defibrillation studies
- Metabolism and Genetic Disorders
- Diabetes Treatment and Management
- Epilepsy research and treatment
- Diabetes Management and Research
- Cardiac Health and Mental Health
Aarhus University Hospital
2013-2024
Aarhus University
2016-2022
Aarhus School of Architecture
2020
Background: Myocardial utilization of 3-hydroxybutyrate (3-OHB) is increased in patients with heart failure and reduced ejection fraction (HFrEF). However, the cardiovascular effects circulating plasma-3-OHB levels these are unknown. Consequently, authors’ aim was to modulate 3-OHB HFrEF evaluate: (1) changes cardiac output (CO); (2) a potential dose-response relationship between CO; (3) impact on myocardial external energy efficiency (MEE) oxygen consumption (MVO 2 ); (4) whether response...
Background The heart can metabolize the microbiota‐derived short‐chain fatty acid butyrate. Butyrate may have beneficial effects in failure, but underlying mechanisms are unknown. We tested hypothesis that butyrate elevates cardiac output by involving direct stimulation of contractility and vasorelaxation rats. Methods Results examined on (1) vivo hemodynamics using parallel echocardiographic invasive blood pressure measurements, (2) isolated perfused hearts Langendorff systems under...
Purpose: It is well established that high-load resistance exercise (HLRE) can stimulate myofibrillar accretion. Additionally, recent studies suggest HLRE also mitochondrial biogenesis and respiratory function. However, in several clinical situations, the use of with high loading may not constitute a viable approach. Low-load blood flow restricted (BFRRE) has emerged as time-effective low-load alternative to unknown if BFRRE If so, could provide feasible strategy muscle metabolic health....
Abstract Normothermic regional perfusion (NRP) allows assessment of therapeutic interventions prior to donation after circulatory death transplantation. Sodium-3-hydroxybutyrate (3-OHB) increases cardiac output in heart failure patients and diminishes ischemia–reperfusion injury, presumably by improving mitochondrial metabolism. We investigated effects 3-OHB on function transplanted hearts organoids. Donor pigs (n = 14) underwent followed NRP. Following static cold storage, were into...
The aim of this study was to determine if hyperpolarized [1,4-
Patients with congestive heart failure (CHF) have impaired functional capacity and inferior quality of life. The clinical manifestations are associated structural impairments in skeletal muscle, emphasizing a need for feasible rehabilitation strategies beyond optimal anticongestive medical treatment. We investigated whether low-load blood flow restricted resistance exercise (BFRRE) or remote ischemic conditioning (RIC) could improve life patients CHF stimulate muscle myofibrillar...
Pre-ischaemic administration of aminooxiacetate (AOA), an inhibitor the malate-aspartate shuttle (MAS), provides cardioprotection against ischaemia-reperfusion injury. The underlying mechanism remains unknown. We examined whether transient inhibition MAS during ischaemia and early reperfusion by AOA treatment could prevent mitochondrial damage at later reperfusion. preserved respiratory capacity with reduced oxidative stress late to same extent as ischaemic preconditioning (IPC). However,...
The mechanisms underlying increased mortality in patients with diabetes and admission hyperglycemia after an acute coronary syndrome may involve reduced capacity for cardioprotection. We investigated the impact of on exogenously activated cardioprotection by ischemic preconditioning (IPC) hearts from rats type 2 mellitus (T2DM) that were endogenously cardioprotected inherent mechanism, involvement myocardial glucose uptake (MGU) O-linked β-N-acetylglucosamine (O-GlcNAc). In isolated,...
The Sodium Glucose Co-Transporter-2 inhibitor, empagliflozin (EMPA), reduces mortality and hospitalisation for heart failure following myocardial infarction irrespective of diabetes status. While the findings suggest an inherent cardioprotective capacity, mechanism remains unknown. We studied infarct size (IS) ex-vivo in isolated hearts exposed to global IR injury in-vivo rats subjected regional ischemia reperfusion (IR) injury, whom we followed left ventricular dysfunction 28 days. compared...
Ischemia reperfusion (IR) injury may be attenuated through succinate dehydrogenase (SDH) inhibition by dimethyl malonate (DiMAL). Whether SDH yields protection in diabetic individuals and translates into human cardiac tissue remain unknown. In isolated perfused hearts from 24 weeks old male Zucker fatty (ZDF) age matched non-diabetic control rats atrial trabeculae patients with without diabetes, we compared infarct size, contractile force recovery mitochondrial function. The cardioprotective...
Hypoglycemia is associated with increased mortality rate in patients diabetes. The underlying mechanisms may involve reduced myocardial tolerance to ischemia and reperfusion (IR) or capacity for ischemic preconditioning (IPC). As IPC glucose uptake (MGU) during reperfusion, cardioprotection linked metabolism possibly by O-linked β-N-acetylglucosamine (O-GlcNAc). We aimed investigate the impact of hypoglycemia hearts from animals diabetes on IR tolerance, efficacy whether modulations MGU...
Remote ischemic conditioning (RIC) protects against sustained myocardial ischemia. Because of overlapping mechanisms, this protection may be altered by glyceryl trinitrate (GTN), which is commonly used in the treatment patients with chronic heart disease. We investigated whether long-term GTN modifies RIC rat myocardium and human endothelium. studied infarct size (IS) hearts subjected to global ischemia-reperfusion (I/R) vitro endothelial function healthy volunteers I/R upper arm. In...
Hepatic and renal energy status prior to transplantation correlates with graft survival. However, effects of brain death (BD) on organ-specific are largely unknown. We studied metabolism, perfusion, oxygen consumption, mitochondrial function in the liver kidneys following BD. BD was induced mechanically-ventilated rats, inflating an epidurally-placed Fogarty-catheter, sham-operated rats as controls. A 9.4T-preclinical MRI system measured hourly availability (BOLD-related R2*) perfusion...
Background Mutations in ATP1A2 gene encoding the Na,K‐ATPase α 2 isoform are associated with familial hemiplegic migraine type 2. Migraine aura is a known risk factor for heart disease. The important cardiac function, but its role disease remains unknown. We hypothesized that susceptibility and aimed to assess underlying mechanism. Methods Results Mice heterozygous 2–associated G301R mutation Atp1a2 (α +/G301R mice) matching wild‐type controls were compared. Reduced expression of increased 1...
Augmented mortality and morbidity following an acute myocardial infarction in patients with diabetes mellitus Type 2 (T2DM) may be caused by increased sensitivity to ischemia reperfusion (IR) injury or altered activation of endogenous cardioprotective pathways modified T2DM per se ischemic preconditioning (IPC). We aimed investigate, whether the duration influences against IR efficacy IPC, how glucose oxidation rate was involved. Male Zucker diabetic fatty rats (homozygote (fa/fa)) at ages...
The measurement of mitochondrial content is essential for bioenergetic research, as it provides a tool to evaluate whether changes in function are strictly due or other mechanisms that influence function. In this perspective, we argue commonly used biomarkers may possess limited utility capturing with physiological intervention. Moreover, they not provide reliable estimates certain pathological situations. Finally, discuss potential solutions overcome issues related the utilization content....
We investigated metabolic changes during brain death (BD) using hyperpolarized magnetic resonance (MR) spectroscopy and ex vivo graft glucose metabolism normothermic isolated perfused kidney (IPK) machine perfusion. BD was induced in mechanically ventilated rats by inflation of an epidurally placed catheter; sham-operated served as controls. Hyperpolarized [1-13 C]pyruvate MR performed to quantify pyruvate the liver kidneys at 3 time points BD, preceded injecting hyperpolarized[1-13...
Background:The Sodium Glucose Transporter 2 (SGLT2)-inhibitor, empagliflozin, reduces death from cardiovascular causes.We hypothesized that the mechanism involved direct protection against Ischemia-Reperfusion (IR) injury and improved post-ischemic mitochondrial function. Methods:We examined infarct size (series I) respiration II) in four groups of isolated perfused hearts male Wistar rats: Sham-operated (Sham group), IR-injured (IR treated with ischemic preconditioning (IPC) by × 5...
The voltage-gated KV7 (KCNQ) potassium channels are activated by ischemia and involved in hypoxic vasodilatation. We investigated the effect of channel modulation on cardiac reperfusion injury its interaction with cardioprotection ischemic preconditioning (IPC). Reverse-transcription polymerase chain reaction revealed expression KV7.1, KV7.4, KV7.5 left anterior descending rat coronary artery all subtypes (KV7.1-KV7.5) right ventricles heart. Isolated hearts were subjected to no-flow global...
Mitochondrial dysfunction has been implicated as a central mechanism in the metabolic myopathy accompanying critical limb ischemia (CLI). However, whether mitochondrial is directly related to lower extremity and structural molecular mechanisms underpinning CLI patients not understood. Here, we aimed study distinctive characteristic of by assessing respiration gastrocnemius muscle from 14 (65.3 ± 7.8 y) 15 matched control (CON) with similar comorbidity risk profile medication regimen but...