A5000575733- Organ Transplantation Techniques and Outcomes
- Organ Donation and Transplantation
- Renal Transplantation Outcomes and Treatments
- Liver Disease and Transplantation
- Transplantation: Methods and Outcomes
- Sulfur Compounds in Biology
- Clinical Nutrition and Gastroenterology
- Renal and Vascular Pathologies
- Anesthesia and Neurotoxicity Research
- Cardiac Ischemia and Reperfusion
- Metabolism and Genetic Disorders
- Neurological Complications and Syndromes
- Liver Disease Diagnosis and Treatment
- Tissue Engineering and Regenerative Medicine
- Acute Kidney Injury Research
- Complement system in diseases
- Mesenchymal stem cell research
- Dialysis and Renal Disease Management
- Pharmacological Effects and Toxicity Studies
- Pancreatic function and diabetes
- Thermal Regulation in Medicine
- Neuroinflammation and Neurodegeneration Mechanisms
- Chronic Kidney Disease and Diabetes
- Folate and B Vitamins Research
- Heme Oxygenase-1 and Carbon Monoxide
University Medical Center Groningen
2016-2025
University of Groningen
2016-2025
European Society for Organ Transplantation
2011-2022
Hanze University of Applied Sciences
2022
Erasmus MC
2017
Erasmus University Rotterdam
2017
Pontificia Universidad Católica de Chile
2016
Dialyse Centrum Groningen
2014
University Medical Centre Mannheim
2014
Heidelberg University
2014
Static cold storage is generally used to preserve kidney allografts from deceased donors. Hypothermic machine perfusion may improve outcomes after transplantation, but few sufficiently powered prospective studies have addressed this possibility.In international randomized, controlled trial, we randomly assigned one 336 consecutive donors and the other storage. All 672 recipients were followed for 1 year. The primary end point was delayed graft function (requiring dialysis in first week...
In Brief Objective: Hypothermic machine perfusion may improve outcome after transplantation of kidneys donated cardiac death (DCD), but no sufficiently powered prospective studies have been reported. Because organ shortage has led to an increased use DCD kidneys, we aimed compare hypothermic with the current standard static cold storage preservation. Methods: Eighty-two kidney pairs from consecutive, controlled donors 16 years or older were included in this randomized trial Eurotransplant....
Hydrogen sulfide (H2S) is an endogenous gasotransmitter with physiologic functions similar to nitric oxide and carbon monoxide. Exogenous treatment H2S can induce a reversible hypometabolic state, which protect organs from ischemia/reperfusion injury, but whether cystathionine γ-lyase (CSE), produces H2S, has protective effects unknown. Here, human renal tissue revealed abundant expression of CSE, localized glomeruli the tubulointerstitium. Compared wild-type mice, CSE knockout mice had...
Unexpected donation after cardiac death (DCD) donors suffer arrest suddenly and are maintained with normothermic extracorporeal membrane oxygenation (NECMO) while consent for is obtained. The objective of this study was to determine whether ex vivo machine perfusion (NMP) improves upon the benefits NECMO in a large-animal model unexpected DCD liver transplant.Donor pigs underwent 90-minute were divided 3 groups. In first, livers preserved immediately cold storage (CS, n = 6). other 2 groups,...
Although normothermic machine perfusion of donor livers may allow assessment graft viability prior to transplantation, there are currently no data on what would be a good parameter viability. To determine whether bile production is suitable biomarker that can used discriminate viable from non-viable we have studied functional performance as well biochemical and histological evidence hepatobiliary injury during ex vivo human livers. After median duration cold storage 6.5 h, twelve extended...
Vascular renal resistance (RR) during hypothermic machine perfusion (HMP) is frequently used in kidney graft quality assessment. However, the association between RR and outcome has never been prospectively validated. Prospectively collected values of 302 machine-perfused deceased donor kidneys all types (standard extended criteria donated after cardiac death), transplanted without prior knowledge these values, were studied. In this cohort, we determined delayed function (DGF) 1-year...
Hydrogen sulfide (H(2)S) can induce a hypometabolic, hibernation-like state in mammals when given subtoxic concentrations. Pharmacologically reducing the demand for oxygen is promising strategy to minimize unavoidable hypoxia-induced injury such as ischemia/reperfusion during renal transplantation. Here we show that H(2)S reduces metabolism vivo, ex and vitro. Furthermore, demonstrate beneficial effects of H(2)S-induced hypometabolism model bilateral using three different treatment...
Increased lung cell apoptosis and necrosis occur in patients with chronic obstructive pulmonary disease (COPD). Mitochondria are crucially involved the regulation of these death processes. Cigarette smoke is main risk factor for development COPD. We hypothesized that cigarette disturbs mitochondrial function, thereby decreasing capacity mitochondria ATP synthesis, leading to cellular necrosis. This hypothesis was tested both human bronchial epithelial cells isolated mitochondria. extract...
Background Livers derived from donation after circulatory death (DCD) are increasingly accepted for transplantation. However, DCD livers suffer additional donor warm ischemia, leading to biliary injury and more complications It is unknown whether oxygenated machine perfusion results in better preservation of epithelium the peribiliary vasculature. We compared hypothermic (HMP) with static cold storage (SCS) a porcine model. Methods After 30 min cardiac arrest, were perfused situ HTK solution...
The mechanism through which oxygenated hypothermic machine perfusion (HMP) improves viability of human extended criteria donor (ECD) livers is not well known. Aim this study was to examine the benefits HMP after static cold storage (SCS).Eighteen ECD that were declined for transplantation underwent ex situ testing using normothermic (37 °C) (NMP) traditional SCS (0 °C-4 7 9 hours. In intervention group (n = 6), 2 hours (at 12 and before NMP. Twelve control NMP without SCS.During HMP, hepatic...
In the last few decades, strategies to improve allograft survival after kidney transplantation have been directed recipient-dependent mechanisms of renal injury. contrast, no such efforts made optimize organ quality in donor. Optimizing deceased donor opens new possibilities outcome.A total 554 biopsies were taken from donation brain death (DBD) and cardiac (DCD) kidneys before donation, cold ischemia reperfusion. Healthy living served as controls. Transcriptomics was performed by whole...
Introduction In the past decades, short-term results after solid organ transplantation have markedly improved. Disappointingly, this has not been accompanied by parallel improvements in long-term outcomes transplantation. To improve graft and recipient outcomes, identification of potentially modifiable risk factors development biomarkers are required. We provide rationale design a large prospective cohort study transplant recipients (TransplantLines). Methods analysis TransplantLines is...
Background and Purpose Hypertension is an important mediator of cardiac damage remodelling. Hydrogen sulfide ( H 2 S ) endogenously produced gasotransmitter with cardioprotective properties. However, it not yet in clinical use. We, therefore, investigated the protective effects sodium thiosulfate STS ), a clinically applicable donor substance, angiotensin II A ng )‐induced hypertensive disease rats. Experimental Approach M ale prague D awley rats were infused (435 kg min −1 or saline...
Adoptive cell transfer of ex vivo expanded regulatory T cells (Tregs) has shown immense potential in animal models auto- and alloimmunity. However, the effective translation such Treg therapies to clinic been slow. Because homeostasis is known require continuous receptor (TCR) ligation exogenous interleukin-2 (IL-2), some investigators have explored use low-dose IL-2 injections increase endogenous responses. Systemic immunotherapy, however, can also lead activation cytotoxic lymphocytes...
Background. After kidney transplantation, decreased graft survival is seen in grafts from brain dead (BD) donors compared with living donors. This might result partly a progressive nonspecific inflammation the graft. In this study, we focused on effects of BD inflammatory response (adhesion molecules, leukocyte invasion, gene expression) and stress-related heat shock proteins human kidney. Research outcomes clinical donor parameters were then linked to outcome data after transplantation....
Recombinant human erythropoietin (rhEPO) is receiving increasing attention as a potential therapy for prevention of injury and restoration function in nonhematopoietic tissues. However, the minimum effective dose required to mimic augment these normal paracrine functions (EPO) some organs (e.g., brain) higher than treatment anemia. Notably, dose-dependent risk adverse effects has been associated with rhEPO administration, especially high-risk groups, including polycythemia–hyperviscosity...
Retrospective evidence suggests that lactate dehydrogenase, aspartate aminotransferase, total glutathione-S-transferase (GST), alanine-aminopeptidase, N-acetyl-β-D-glucosaminidase (NAG), and heart-type fatty acid binding protein (H-FABP) measured during kidney machine perfusion (MP) could have predictive value for posttransplant outcome. However, these data may be biased due to organ discard based on biomarker measurements, previous analyses were not adjusted likely confounding factors. No...