A5019456840- Organ Transplantation Techniques and Outcomes
- Organ Donation and Transplantation
- Renal Transplantation Outcomes and Treatments
- Liver Disease and Transplantation
- Renal and Vascular Pathologies
- Tissue Engineering and Regenerative Medicine
- Transplantation: Methods and Outcomes
- Mesenchymal stem cell research
- MRI in cancer diagnosis
- Advanced MRI Techniques and Applications
- Ultrasound and Hyperthermia Applications
- Acute Kidney Injury Research
- Dialysis and Renal Disease Management
- Electrospun Nanofibers in Biomedical Applications
- Cardiac Ischemia and Reperfusion
- Blood donation and transfusion practices
- Animal Behavior and Welfare Studies
- Renal and related cancers
- Body Composition Measurement Techniques
- Abdominal vascular conditions and treatments
- Hemoglobin structure and function
- Complement system in diseases
- Xenotransplantation and immune response
- Infectious Aortic and Vascular Conditions
- Veterinary Pharmacology and Anesthesia
University Medical Center Groningen
2016-2025
University of Groningen
2011-2024
European Society for Organ Transplantation
2011-2022
GTx (United States)
2016
Eurotransplant
2008
Static cold storage is generally used to preserve kidney allografts from deceased donors. Hypothermic machine perfusion may improve outcomes after transplantation, but few sufficiently powered prospective studies have addressed this possibility.In international randomized, controlled trial, we randomly assigned one 336 consecutive donors and the other storage. All 672 recipients were followed for 1 year. The primary end point was delayed graft function (requiring dialysis in first week...
In Brief Objective: Hypothermic machine perfusion may improve outcome after transplantation of kidneys donated cardiac death (DCD), but no sufficiently powered prospective studies have been reported. Because organ shortage has led to an increased use DCD kidneys, we aimed compare hypothermic with the current standard static cold storage preservation. Methods: Eighty-two kidney pairs from consecutive, controlled donors 16 years or older were included in this randomized trial Eurotransplant....
Liver transplantation is traditionally performed around the clock to minimize organ ischemic time. However, prospect of prolonging preservation times holds potential streamline logistics and transform liver into a semi-elective procedure, reducing need for nighttime surgeries. Dual hypothermic oxygenated machine perfusion (DHOPE) donor livers 1-2 h mitigates ischemia-reperfusion injury improves transplant outcomes. Preclinical studies have shown that DHOPE can safely extend up 24 h.
Vascular renal resistance (RR) during hypothermic machine perfusion (HMP) is frequently used in kidney graft quality assessment. However, the association between RR and outcome has never been prospectively validated. Prospectively collected values of 302 machine-perfused deceased donor kidneys all types (standard extended criteria donated after cardiac death), transplanted without prior knowledge these values, were studied. In this cohort, we determined delayed function (DGF) 1-year...
Retrospective evidence suggests that lactate dehydrogenase, aspartate aminotransferase, total glutathione-S-transferase (GST), alanine-aminopeptidase, N-acetyl-β-D-glucosaminidase (NAG), and heart-type fatty acid binding protein (H-FABP) measured during kidney machine perfusion (MP) could have predictive value for posttransplant outcome. However, these data may be biased due to organ discard based on biomarker measurements, previous analyses were not adjusted likely confounding factors. No...
Background. Acute rejection after renal transplantation has been shown to be negatively associated with long-term graft survival. Identifying donor factors that are acute in the recipient could help a better understanding of relevant underlying processes lead injury. Complement activation an important mediator transplant related In this study, we analyzed effect systemic complement deceased donors before their kidneys on posttransplant outcome recipient. Methods. Plasma from 232 brain-dead...
Static cold storage (CS) is the most widely used organ preservation method for deceased donor kidney grafts but there increasing evidence that hypothermic machine perfusion (MP) may result in better outcome after transplantation. We performed an economic evaluation of MP versus CS alongside a multicenter RCT investigating short- and long-term cost-effectiveness. Three hundred thirty-six consecutive pairs were included, one which was assigned to CS. The combined short-term results based on...
In Brief Background Hypothermic machine perfusion (HMP) of deceased donor kidneys is associated with better outcome when compared to static cold storage (CS). Nevertheless, there little evidence whether short ischemia time (CIT) also benefit from HMP and can safely extend CIT. Methods We analyzed prospectively collected data the Machine Preservation Trial, an international randomized controlled trial. Seven hundred fifty-two consecutive renal transplants were included: 1 kidney each 376...
Hypothermic machine perfusion (HMP) has become standard care in many center's to preserve kidneys donated after circulatory death (DCD). Despite a significant reduction metabolism at low temperatures, the remaining cellular activity requires oxygen. Because of role and safety oxygen during HMP not been fully clarified, its supply is yet. This study investigates effect administering on renal function porcine DCD model.After 30 minutes warm ischemia, slaughterhouse were preserved for 24 hours...
Normothermic machine perfusion (NMP) of kidneys offers the opportunity to perform active interventions, such as addition mesenchymal stromal cells (MSCs), an isolated organ prior transplantation. The purpose this study was determine whether administering MSCs during NMP is feasible, what effect on and intact are retained in kidney which structures they home. Viable porcine were obtained from a slaughterhouse. Kidneys perfused 7 h at 37 °C. After 1 either 0, 105, 106 or 107 human adipose...
Organ gene therapy represents a promising tool to correct diseases or improve graft survival after transplantation. Polymorphic variation of the major histocompatibility complex (MHC) antigens remains obstacle long-term Previously, we demonstrated that MHC-silenced cells are protected against allogeneic immune responses. We also showed feasibility silence MHC in lung. Here, aimed at genetic engineering kidney toward permanent silencing rat model. constructed sub-normothermic ex vivo...
Renal ex vivo normothermic machine perfusion (NMP) is under development as an assessment tool for high-risk kidney grafts and a means of achieving more physiologically accurate organ preservation. On-going hemolysis has been reported during NMP, this technique relies on red blood cells oxygen delivery. In study, we confirm the occurrence progressive 6-hour NMP. NMP-associated erythrostasis in glomeruli peri-glomerular vascular networks points to interaction between graft. Continuous resulted...
Background.In the Eurotransplant Senior Programme (ESP), kidneys from donors aged ≥65 years are preferentially allocated locally and transplanted into patients on dialysis.The purpose of this study was to analyse whether results transplantation in ESP can be improved by preservation organs hypothermic machine perfusion (MP) compared with simple cold storage (CS).Methods.Overall, 85 deceased heart-beating age were included analysis follow-up until 1 year post-transplant.For each donor, one...
Normothermic machine perfusion (NMP) of injured kidneys offers the opportunity for interventions to metabolically active organs prior transplantation. Mesenchymal stromal cells (MSCs) can exert regenerative and anti-inflammatory effects in ischemia-reperfusion injury. The aims this study were evaluate safety feasibility MSC treatment during NMP using a porcine autotransplantation model, examine potential treatment-associated kidney improvements up 14 days posttransplant. After 75 min warm...
In experimental research, animal welfare should always be of the highest priority. Currently, physical in-person observations are standard. This is time-consuming, and results subjective. Video-based machine learning models for monitoring pigs provide a continuous objective observation method misthriving detection. The aim this study was to develop validate pig tracking technology, using video-based data in model analyze posture activity level living single-pig pens. A research prototype...
Background. Transplantation of older deceased donor kidneys is gaining wide acceptance in most countries. Many previous studies have concluded that advanced age negatively impacts posttransplant outcome, but detailed data on the extent to which a few years increase will influence early graft function and survival are scarce. Methods. We used Organ Procurement Network database (cohort 1994–2006, n=99,860 recipients) evaluate effect results obtain regression models relevant guide clinical...
Pretransplant normothermic machine perfusion (NMP) of donor kidneys offers the unique opportunity to perform active interventions an isolated renal graft before transplantation. There is increasing evidence that mesenchymal stromal cells (MSCs) could have a paracrine/endocrine regenerative effect on ischemia-reperfusion injury. The purpose this study was determine which cytokines are secreted by MSCs during NMP porcine kidney. Viable and autologous whole blood were obtained from...
Acceptance criteria of deceased donor organs have gradually been extended toward suboptimal quality, posing an urgent need for more objective pre-transplant organ assessment. Ex vivo normothermic machine perfusion (NMP) combined with magnetic resonance imaging (MRI) could assist clinicians in deciding whether a kidney is suitable transplantation. Aim this study was to characterize the regional distribution perfusate flow during NMP, better understand how ex assessment protocols should...