Rianne M. Douwes
A5037448433- Renal Transplantation Outcomes and Treatments
- Pharmacological Effects and Toxicity Studies
- Gut microbiota and health
- Palliative Care and End-of-Life Issues
- Dialysis and Renal Disease Management
- Potassium and Related Disorders
- Organ Transplantation Techniques and Outcomes
- Iron Metabolism and Disorders
- Diet and metabolism studies
- Nutrition and Health in Aging
- Hemoglobinopathies and Related Disorders
- Clinical Nutrition and Gastroenterology
- Hydrogen's biological and therapeutic effects
- Trace Elements in Health
- Folate and B Vitamins Research
- Dysphagia Assessment and Management
- Liver Disease and Transplantation
- Nutritional Studies and Diet
- Organ Donation and Transplantation
- Liver Disease Diagnosis and Treatment
- Transplantation: Methods and Outcomes
- Neurological Complications and Syndromes
- Metabolism and Genetic Disorders
- Kidney Stones and Urolithiasis Treatments
- Cardiovascular Issues in Pregnancy
University of Groningen
2017-2023
University Medical Center Groningen
2017-2023
Introduction In the past decades, short-term results after solid organ transplantation have markedly improved. Disappointingly, this has not been accompanied by parallel improvements in long-term outcomes transplantation. To improve graft and recipient outcomes, identification of potentially modifiable risk factors development biomarkers are required. We provide rationale design a large prospective cohort study transplant recipients (TransplantLines). Methods analysis TransplantLines is...
Renal transplantation is life-changing in many aspects. This includes changes to the gut microbiome likely due exposure immunosuppressive drugs and antibiotics. As a consequence, renal transplant recipients (RTRs) might suffer from intestinal dysbiosis. We aimed investigate of RTRs compare it with healthy controls identify determinants RTRs. Therefore, participating TransplantLines Biobank Cohort Study (NCT03272841) were included. analyzed using 16S rRNA sequencing compared composition...
Organ transplantation is a life-saving treatment for patients with end-stage disease, but survival rates after vary considerably. There now increasing evidence that the gut microbiome linked to of undergoing hematopoietic cell transplant, yet little known about role in solid organ transplantation. We analyzed 1370 fecal samples from 415 liver and 672 renal transplant recipients using shotgun metagenomic sequencing assess microbial taxonomy, metabolic pathways, antibiotic resistance genes,...
The influence of dairy on the gut microbiome has not been studied extensively. We performed a randomized cross-over study to analyze effect high intake microbiome. Subjects were randomly assigned high-dairy diet (HDD) (5-6 portions per day) and low-dairy (LDD) (≤1 portion for 6 weeks with washout period 4 in between both diets. was assessed using 16S rRNA gene sequencing. Compositionality functionality Quantitative Insights Into Microbial Ecology (QIIME) Phylogenetic Investigation...
Calciprotein particles (CPPs) are circulating calcium and phosphate nanoparticles associated with the development of vascular calcification (VC) in chronic kidney disease (CKD). Although recent studies have been focusing on associations CPPs presence VC CKD, insights underlying processes mechanisms by which might aggravate dysfunction vivo currently lacking. Here, we assessed overall burden abdominal healthy donors CKD patients subsequently performed transcriptome profiling tissue obtained...
Proton-pump inhibitors (PPIs) are commonly used after kidney transplantation and there is rarely an incentive to discontinue treatment. In the general population, PPI use has been associated with hypomagnesaemia. We aimed investigate whether plasma magnesium, 24-h urinary magnesium excretion hypomagnesaemia, in transplant recipients (KTR). Plasma were measured 686 stable outpatient KTR a functioning allograft for ≥1 year from TransplantLines Food Nutrition Biobank Cohort-Study (NCT02811835)....
Abstract Background Type 2 diabetes is associated with both impaired insulin action at target tissues and secretion in pancreatic beta cells. Mitochondrial dysfunction may play a role resistance secretion. Plasma creatine has been proposed as potential marker for mitochondrial dysfunction. We aimed to investigate the association between plasma incident type diabetes. Methods measured fasting concentrations by nuclear magnetic resonance spectroscopy participants of general population‐based...
Proton-pump inhibitor (PPI) use may influence intestinal iron absorption. Low status and deficiency (ID) are frequent medical problems in renal transplant recipients (RTR). We hypothesized that chronic PPI is associated with lower ID RTR. Serum iron, ferritin, transferrin saturation (TSAT), hemoglobin were measured 646 stable outpatient RTR a functioning allograft for ≥ 1 year from the "TransplantLines Food Nutrition Biobank Cohort Study" (NCT02811835). Median time since transplantation was...
Background and objectives Many kidney transplant recipients suffer from fatigue poor health-related quality of life. Airflow limitation may be an underappreciated comorbidity among recipients, which could contribute to lower life in this population. In study, we compared the prevalence airflow between healthy controls investigated associations with recipients. Design, setting, participants, & measurements Data ongoing TransplantLines Biobank Cohort study were used. was defined as forced...
Background Chronic use of proton-pump inhibitors (PPIs) is common in kidney transplant recipients (KTRs). However, concerns are emerging about the potential long-term complications PPI therapy. We aimed to investigate whether associated with excess mortality risk KTRs. Methods and findings investigated association using multivariable Cox proportional hazard regression analyses a single-center prospective cohort 703 stable outpatient KTRs, who visited clinic University Medical Center...
Abstract In kidney transplantation, microthrombi and fibrin deposition may lead to local perfusion disorders subsequently poor initial graft function. Microthrombi are often regarded as donor-derived. However, the incidence, time of development, potential difference between living donor kidneys (LDK) deceased kidneys(DDK), remains unclear. Two open-needle biopsies, taken at preimplantation after reperfusion, were obtained from 17 LDK 28 DDK transplanted 2005 2008. Paraffin-embedded sections...
Kidney transplant recipients (KTRs) experience more fatigue, anxiety, and depressive symptoms lower concentration health-related quality of life (HRQoL) compared with the general population. Anemia is a potential cause that well-recognized treated. Iron deficiency, however, often unrecognized, despite its detrimental effects related to unrelated anemia. We investigated interplay anemia, iron patient-reported outcomes in 814 outpatient KTRs (62% male, age 56 ± 13 years) enrolled...
Gut dysbiosis has been associated with impaired outcomes in liver and kidney transplant recipients, but the gut microbiome of lung recipients not extensively explored. We assessed 64 fecal samples from end-stage disease patients before transplantation 219 after using metagenomic sequencing. To identify dysbiotic microbial signatures, we analyzed 243 age-, sex-, BMI-matched healthy controls. By unsupervised clustering, identified five groups different combinations immunosuppressants...
Decreased circulating branched chain amino acids (BCAA) represent a prominent change in acid profiles patients with end-stage liver disease (ESLD). These alterations are considered to contribute sarcopenia and hepatic encephalopathy may relate poor prognosis. Here, we cross-sectionally analyzed the association between plasma BCAA levels severity of ESLD muscle function participants transplant subgroup TransplantLines, enrolled January 2017 2020. Plasma were measured by nuclear magnetic...
Epidemiologic studies have linked urinary oxalate excretion to risk of chronic kidney disease (CKD) progression and end-stage renal disease. We aimed investigate whether oxalate, in stable transplant recipients (KTR), is prospectively associated with graft failure. In secondary analyses we evaluated the association post-transplantation diabetes mellitus, all-cause mortality specific causes death. Oxalate was measured 24-h urine collection samples a cohort 683 KTR functioning allograft ≥1...
Post-transplantation diabetes mellitus (PTDM) is a major clinical problem in kidney transplant recipients (KTRs). Diuretic-induced hyperglycaemia and have been described the general population. We aimed to investigate whether diuretics also increase PTDM risk KTRs.We included 486 stable outpatient KTRs (with functioning graft ≥1 year) without from prospective cohort study. Participants were classified as diuretic users non-users based on their medication use verified by medical records.At...
Heavy metals are known to induce kidney damage, and recent studies have linked minor exposures cadmium arsenic with increased risk of allograft failure, yet the potential association lead late graft failure in transplant recipients (KTRs) remains unknown.Prospective cohort study The Netherlands.We studied outpatient KTRs (n = 670) a functioning for ≥1 year recruited at university setting (2008-2011) followed median 4.9 (interquartile range, 3.4-5.5) years. Additionally, patients chronic...
Prior studies report that the use of proton pump inhibitors (PPIs) can adversely affect gut microbiota and gastrointestinal uptake micronutrients, in particular iron magnesium, are used frequently by kidney transplant recipients. Altered microbiota, deficiency, magnesium deficiency have been implicated pathogenesis chronic fatigue. Therefore, we hypothesized PPI may be an important underappreciated cause fatigue reduced health-related quality life (HRQoL) this population.Cross-sectional...
Impaired interplay between sensory and motor function may be an important, often overlooked cause of the decreased daily functioning impaired health-related quality life (HRQoL) kidney transplant recipients (KTR). We assessed this using a hand dexterity test, investigated its potential associations with HRQoL among KTR enrolled at TransplantLines Biobank Cohort Study. A total 309 (58% male, mean age 56 ± 13 years) median 4 [IQR: 1-11] years after transplantation were included. dexterity, as...
Renal transplant recipients (RTRs) often suffer from posttransplant diarrhea. The observed dysbiosis in RTR may influence the fermentation processes gut. In this study, we aimed to investigate whether differs between RTRs and healthy controls (HCs), by measuring breath H2 CH4 concentrations. Additionally, determined fecal presence of methanogen Methanobrevibacter smithii (M. smithii), which plays a main role process methanogenesis. Data TransplantLines Biobank Cohort Study (NCT03272841) was...
In a search for potentially modifiable factors to improve long-term outcome among kidney transplant recipients (KTR), we hypothesized that boron exposure is associated with improved in KTR.We determined 24 h urinary excretion using inductively coupled plasma mass spectrometry as measure of 693 stable KTR (57% male, mean age 53y), enrolled the TransplantLines Food and Nutrition Biobank Cohort Study. Dietary intake was assessed validated food-frequency questionnaires.Linear regression analyses...