A5037513398- Ion Transport and Channel Regulation
- Ion channel regulation and function
- Enzyme Structure and Function
- ATP Synthase and ATPases Research
- Enzyme Production and Characterization
- RNA Research and Splicing
- Extracellular vesicles in disease
- Magnesium in Health and Disease
- Plant Micronutrient Interactions and Effects
- RNA and protein synthesis mechanisms
- Membrane-based Ion Separation Techniques
- Glycosylation and Glycoproteins Research
- Biochemical and Molecular Research
- Mitochondrial Function and Pathology
- Drug Transport and Resistance Mechanisms
- Lipid Membrane Structure and Behavior
- Circular RNAs in diseases
- Trace Elements in Health
- Peptidase Inhibition and Analysis
- Protein Structure and Dynamics
- Plant Stress Responses and Tolerance
- Bacterial Genetics and Biotechnology
- Polyamine Metabolism and Applications
- Bacteriophages and microbial interactions
- Carbohydrate Chemistry and Synthesis
Technical University of Denmark
2016-2025
Oslo University Hospital
2012-2023
University of Oslo
2013-2023
Institutt for Eksperimentell Medisinsk Forskning
2011-2023
Aarhus University
2006-2014
Nord University
2013
Danish National Research Foundation
2007-2011
University of California, San Francisco
2011
National Research Foundation
2010
Graduate School USA
2009
Abstract Exosomes are small secreted vesicles that can transfer their content to recipient cells. In cancer, exosome secretion has been implicated in tumor growth and metastatic spread. this study, we explored the possibility exosomal pathways might discard tumor-suppressor miRNA restricts progression. Secreted characterized from isogenic bladder carcinoma cell lines with differing potential were uncoupled binding target transcripts or AGO2–miRISC complex. cells, observed a relative increase...
The mucolytic human gut microbiota specialist Akkermansia muciniphila is proposed to boost mucin-secretion by the host, thereby being a key player in mucus turnover. Mucin glycan utilization requires removal of protective caps, notably fucose and sialic acid, but enzymatic details this process remain largely unknown. Here, we describe specificities ten A. glycoside hydrolases, which collectively remove all known sialyl fucosyl mucin caps including those on double-sulfated epitopes....
P-type ATPases are a large family of integral membrane transporters that vital importance in all kingdoms life. In eponymous distinction from the other main classes transport ATPase – F0F1 (F-), vacuolar (V-) and ATP-binding cassette (ABC-) type form phosphorylated (P-) intermediate state during their ion cycle (Pedersen Carafoli, 1987). Members this generate maintain crucial (electro-) chemical gradients across cellular membranes, by translocating cations, heavy metals lipids.The...
Tankyrases 1 and 2 (TNKS1/2) are promising pharmacological biotargets with possible applications for the development of novel anticancer therapeutics. A focused structure–activity relationship study was conducted based on tankyrase inhibitor JW74 (1). Chemical analoging improved 1,2,4-triazole core led to 4-{5-[(E)-2-{4-(2-chlorophenyl)-5-[5-(methylsulfonyl)pyridin-2-yl]-4H-1,2,4-triazol-3-yl}ethenyl]-1,3,4-oxadiazol-2-yl}benzonitrile (G007-LK), a potent, "rule 5" compliant metabolically...
Abstract Patients with short QT syndrome (SQTS) may present syncope, ventricular fibrillation or sudden cardiac death. Six SQTS susceptibility genes, encoding cation channels, explain <25% of cases. Here we identify a missense mutation in the anion exchanger (AE3)-encoding SLC4A3 gene two unrelated families SQTS. The causes reduced surface expression AE3 and membrane bicarbonate transport. Slc4a3 knockdown zebrafish increased pH i , QTc, systolic duration, which is rescued by wildtype but...
Abstract Improved therapies are needed against snakebite envenoming, which kills and permanently disables thousands of people each year. Recently developed neutralizing monoclonal antibodies several snake toxins have shown promise in preclinical rodent models. Here, we use phage display technology to discover a human antibody show that this causes antibody-dependent enhancement toxicity (ADET) myotoxin II from the venomous pit viper, Bothrops asper , mouse model envenoming mimics snakebite....
We have determined the structure of sarco(endo)plasmic reticulum Ca2+-ATPase (SERCA) in an E2·Pi-like form stabilized as a complex with MgF42−, ATP analog, adenosine 5′-(β,γ-methylene)triphosphate (AMPPCP), and cyclopiazonic acid (CPA). The at 2.5Å resolution leads to significantly revised model CPA binding when compared earlier reports. It shows that divalent metal ion is required for through coordination tetramic moiety characteristic kink M1 helix found all P-type ATPase structures, which...
To obtain the initial crystallization hit and subsequent optimization remain major bottlenecks in membrane protein structure determination. Here we describe an approach to achieve diffraction quality crystals high concentrations of lipid detergent (HILIDE). Initial hits are achieved through a controlled variation protein/lipid/detergent ratios, optimized established by systematic screening with secondary detergents at that adapted for method. The is generally applicable native heterologously...
The aim of this study was to investigate the relationship between aquaporin (AQP) water channel expression and pathological features early untreated inflammatory bowel disease (IBD) in humans.Patients suspected have IBD on basis predefined symptoms, including abdominal pain, diarrhea, and/or blood stool for more than 10 days, were examined at local hospital. Colonoscopy with biopsies performed samples taken. Patients who did not meet diagnostic criteria displayed no evidence infection or...
Rab GTPases are key regulators of intracellular trafficking, and cycle between a GTP-bound active state GDP-bound inactive state. This is regulated by guanine-nucleotide exchange factors (GEFs) GTPase-activating proteins (GAPs). Several efforts have been made in connecting the correct GEFs GAPs to their specific Rab. Here, we aimed identify for Rab7b, small GTPase involved transport from late endosomes trans-Golgi. An siRNA screen targeting containing TBC domains critical was performed...
DsbB is an E. coli membrane protein that oxidizes DsbA, the primary disulfide donor present in periplasm.To understand how bonds are generated and introduced into secreted proteins, we determined crystal structure of a complex with DsbA endogenous ubiquinone at 3.7 Å resolution.The first revealed contains four-helix bundle scaffold transmembrane region one short membrane-parallel α-helix long periplasmic loop.Strikingly, disulfide-generating reaction center composed Cys41, Cys44, Arg48...