A5088126302- Ion Transport and Channel Regulation
- Ion channel regulation and function
- Membrane-based Ion Separation Techniques
- Hormonal Regulation and Hypertension
- ATP Synthase and ATPases Research
- Ion Channels and Receptors
- Lipid Membrane Structure and Behavior
- Mitochondrial Function and Pathology
- Neuroscience and Neuropharmacology Research
- Agriculture Sustainability and Environmental Impact
- Pancreatic function and diabetes
- Neonatal Health and Biochemistry
- Photoreceptor and optogenetics research
- Magnetic and Electromagnetic Effects
- Phosphorus and nutrient management
- Lower Extremity Biomechanics and Pathologies
- Neuroendocrine regulation and behavior
- Bone fractures and treatments
- Hydrogen Storage and Materials
- Malaria Research and Control
- Soil and Water Nutrient Dynamics
- Enzyme Structure and Function
- Insect and Pesticide Research
- Meat and Animal Product Quality
- Advancements in PLL and VCO Technologies
Aarhus University
2013-2022
Danish Diabetes Academy
2019
Danish National Research Foundation
2010-2016
National Research Foundation
2010
Instituto de Ciencias Agrarias
2006
Kennedy Center
1983-1988
The Na(+), K(+)-adenosine triphosphatase (ATPase) maintains the electrochemical gradients of Na(+) and K(+) across plasma membrane--a prerequisite for electrical excitability secondary transport. Hitherto, structural information has been limited to K(+)-bound or ouabain-blocked forms. We present crystal structure a Na(+)-bound K(+)-ATPase as determined at 4.3 Å resolution. Compared with form, large conformational changes are observed in α subunit whereas β γ structures maintained. locations...
Abstract The vital gradients of Na + and K across the plasma membrane animal cells are maintained by Na,K-ATPase, an αβ enzyme complex, whose α subunit carries out ion transport ATP hydrolysis. specific roles β isoforms less clear, though β2 is essential for motor physiology in mammals. Here, we show that compared to β1 β3, stabilizes -occluded E1P state relative outward-open E2P effect mediated its transmembrane domain. Molecular dynamics simulations further demonstrate tilt angle helix...
Hypokalaemic periodic paralysis is a rare genetic neuromuscular disease characterized by episodes of skeletal muscle associated with low serum potassium. Muscle fibre inexcitability during attacks due to an aberrant depolarizing leak current through mutant voltage sensing domains either the sarcolemmal voltage-gated calcium or sodium channel. We report child hypokalaemic and CNS involvement, including seizures, but without mutations in known genes. identified novel heterozygous de novo...
The objective of the study was to estimate Leu requirement for weaned piglets balance indispensable AA in reduced CP diets. A dose-response experiment conducted standardized ileal digestible (SID) Lys ratio required maximum growth young pigs after weaning. In this study, 96 female (initial BW 8 kg) were allotted 1 6 dietary treatments with 16 individually penned per treatment. Graded levels crystalline L-Leu added a basal diet provide diets containing 0.70, 0.80, 0.90, 1.00, 1.10, and 1.20...
In their journey from the male to female reproductive tract, spermatozoa are confronted with a constantly changing environment. To cope associated challenges, express distinct set of transporters, channels and pumps. One membrane proteins unique is α4 isoform Na + ,K ‐ ATP ase. addition α4, ubiquous α1 variant. get detailed understanding how differ, why need an additional ase, we have conducted electrophysiological comparison rodent isoforms (rat versus mouse α1–3) using Xenopus oocyte...
To generate tryptophan-overproducing Bacillus subtilis strains for in situ use pigs, to reduce the feed cost farmers and nitrogen pollution.A novel concept has been investigated-to B. able produce tryptophan (Trp) pigs. Mutagenesis by UV was combined with selection on Trp purine analogues an iterative process. Two mutants from different wild types were obtained, mutant 1 (M1) produced mg Trp/l 2 (M2) 14 Trp/l. Genome sequence analysis revealed that M1 had three single nuclear polymorphisms...
The sole purpose of a sperm cell is to carry genetic information from male female egg. In order accomplish this quest, the must travel long distance through constantly changing environment. success journey depends on membrane proteins that are uniquely expressed in cells. One these α4 isoform sodium pump. This pump optimized cope with ionic environment characteristic reproductive tract, and its activity may be tightly coupled secondary transporters maintain cytoplasmic pH. Pharmacological...
The Na+,K+-ATPase generates electrochemical gradients of Na+ and K+ across the plasma membrane via a functional cycle that includes various phosphoenzyme intermediates. However, structure function these intermediates how metal fluorides mimick them require further investigation. Here, we describe 4.0 Å resolution crystal properties pig kidney stabilized by inhibitor beryllium fluoride (denoted E2-BeFx). E2-BeFx is expected to mimic E2P phosphoenzyme, yet with unknown characteristics ion...
Abstract The tension band plating technique for hemiepiphysiodesis is new and advocated because it believed to reduce the risk of premature closure growth plate compared stapling. benefit has not yet been proven in experimental or randomized clinical studies. Ten weeks old domestic pigs two randomized, paired studies were used. Right proximal tibia was medial epiphysiodesis by either stapling plating. Left side received opposite treatment. Study A ( n = 10): 9 B 8): implant removal after...
The Na,K-ATPase is essential to all animals, since it maintains the electrochemical gradients that energize plasma membrane. Naturally occurring inhibitors of pump from plants have been used pharmaceutically in cardiac treatment for centuries. block by binding on its extracellular side and thereby locking it. To explore possibilities designing an alternative way targeting function, we examined structural requirements a pocket accommodates two C-terminal residues, YY, crystal structures pump....