A5050271585- Physics of Superconductivity and Magnetism
- Advanced Condensed Matter Physics
- Trace Elements in Health
- Drug Transport and Resistance Mechanisms
- Protein Structure and Dynamics
- Magnetic and transport properties of perovskites and related materials
- Magnetic properties of thin films
- Theoretical and Computational Physics
- Metal complexes synthesis and properties
- Photoreceptor and optogenetics research
- Ion channel regulation and function
- Enzyme Structure and Function
- Lipid Membrane Structure and Behavior
- Advanced Thermoelectric Materials and Devices
- Photosynthetic Processes and Mechanisms
- Rare-earth and actinide compounds
- Ion Transport and Channel Regulation
- Neuroscience and Neuropharmacology Research
- Magnetic Properties and Applications
- Microstructure and Mechanical Properties of Steels
- Spectroscopy and Quantum Chemical Studies
- Hydrogen Storage and Materials
- Iron-based superconductors research
- Shape Memory Alloy Transformations
- Bacterial Genetics and Biotechnology
Umeå University
2018-2024
KTH Royal Institute of Technology
2005-2018
Science for Life Laboratory
2013-2018
Swedish e-Science Research Centre
2014-2016
University of California, Irvine
2010-2014
Stockholm University
2002-2014
University of Gothenburg
2011
Interface (United States)
2011
Chalmers University of Technology
2006-2010
European Synchrotron Radiation Facility
2010
The Na(+), K(+)-adenosine triphosphatase (ATPase) maintains the electrochemical gradients of Na(+) and K(+) across plasma membrane--a prerequisite for electrical excitability secondary transport. Hitherto, structural information has been limited to K(+)-bound or ouabain-blocked forms. We present crystal structure a Na(+)-bound K(+)-ATPase as determined at 4.3 Å resolution. Compared with form, large conformational changes are observed in α subunit whereas β γ structures maintained. locations...
ClC-1 protein channels facilitate rapid passage of chloride ions across cellular membranes, thereby orchestrating skeletal muscle excitability. Malfunction is associated with myotonia congenita, a disease impairing relaxation. Here, we present the cryo-electron microscopy (cryo-EM) structure human ClC-1, uncovering an architecture reminiscent that bovine ClC-K and CLC transporters. The conducting pathway exhibits distinct features, including central glutamate residue ("fast gate") known to...
Abstract Copper transporting P-type (P 1B-1 -) ATPases are essential for cellular homeostasis. Nonetheless, the E1-E1P-E2P-E2 states mechanism of P -ATPases remains poorly understood. In particular, role intrinsic metal binding domains (MBDs) is enigmatic. Here, four cryo-EM structures and molecular dynamics simulations a -ATPase combined to reveal that in many eukaryotes MBD immediately prior ATPase core, −1 , serves structural role, remodeling ion-uptake region. contrast, −2 likely assists...
β-Zn4Sb3 is an outstanding thermoelectric material mainly due to its extraordinarily low thermal conductivity, which similar that of glasses. Recently it was proposed interstitial Zn atoms are responsible for this peculiar behavior. Here we report on the crystal and electronic stucture low-temperature polymorph α-Zn4Sb3. During reversible phase transition intricate disorder in disappears, all localize completely. The structure α-Zn4Sb3 corresponds a narrow-gap semiconductor.
Photosynthetic reaction centers convert the energy content of light into a transmembrane potential difference and so provide major pathway for input biosphere. We applied time-resolved Laue diffraction to study light-induced conformational changes in photosynthetic center complex Blastochloris viridis. The side chain TyrL162, which lies adjacent special pair bacteriochlorophyll molecules that are photooxidized primary conversion event photosynthesis, was observed move 1.3 angstroms closer...
Zinc is essential for all organisms and yet detrimental at elevated levels. Hence, homeostasis of this metal tightly regulated. The Zrt/Irt-like proteins (ZIPs) represent the only zinc importers in metazoans. Mutations human ZIPs cause serious disorders, but mechanism by which transfer remains elusive. Hitherto, structural information available a model member, BbZIP, as single, ion-bound conformation, precluding mechanistic insights. Here, we elucidate an inward-open metal-free BbZIP...
The low-temperature phase transitions of thermoelectric Zn4Sb3 have been characterized using single-crystal X-ray diffraction, electrical resistance, and thermal conductivity measurements. Room-temperature stable, disordered β-Zn4Sb3 undergoes a transition at 254 K to ordered α-Zn4Sb3, which has an ideal composition Zn13Sb10. Below 235 K, second (α'-Zn4Sb3) can be detected. sequence β−α−α' is reversible. α−α' transformation originates from slight Zn deficiency with respect actual Zn13-δSb10.
Time-resolved wide-angle x-ray scattering (TR-WAXS) is an emerging biophysical method which probes protein conformational changes with time. Here we present a comparative TR-WAXS study of native green-absorbing proteorhodopsin (pR) from SAR86 and halogenated derivative for the retinal chromophore has been replaced 13-desmethyl-13-iodoretinal (13-I-pR). Transient absorption spectroscopy differences show that 13-I-pR photocycle both accelerated displays more complex kinetics than pR....
Voltage-gated potassium (KV) channels are membrane proteins that respond to changes in potential by enabling K+ ion flux across the membrane. Polyunsaturated fatty acids (PUFAs) induce channel opening modulating voltage-sensitivity, which can provide effective treatment against refractory epilepsy means of a ketogenic diet. While PUFAs have been reported influence gating mechanism electrostatic interactions voltage-sensor domain (VSD), exact PUFA-protein still elusive. In this study, we...
We characterize thus-far elusive domain rearrangements of a calcium-transporting ATPase in the native membrane.
Time-resolved x-ray solution scattering identifies cooperative structural dynamics in the adenylate kinase enzymatic reaction.
Abstract Copper is essential for living cells, yet toxic at elevated concentrations. Class 1B P-type (P -) ATPases are present in all kingdoms of life, facilitating cellular export transition metals including copper. follow an alternating access mechanism, with inward-facing E1 and outward-facing E2 conformations. Nevertheless, no structural information on states available P -ATPases, hampering mechanistic understanding. Here, we structures that reach 2.7 Å resolution a copper-specific...
The small-angle X-ray scattering (SAXS) methodology enables structural characterization of biological macromolecules in solution. However, because SAXS provides low-dimensional information, several potential configurations can reproduce the experimental profile, which severely complicates refinement process. Here, we present a bias-exchange metadynamics protocol that incorporates data as collective variables and therefore tags all possible with their corresponding free energies, allows...
Aquaporin TIP2;1 is a protein channel permeable to both water and ammonia. The structural origin of ammonia selectivity remains obscure, but experiments have revealed that double mutation renders it impermeable without affecting permeability. Here, we aim reproduce explain these observations by performing an extensive mutational study using microsecond long molecular dynamics simulations, applying the two popular force fields CHARMM36 Amber ff99SB-ILDN. We calculate permeabilities free...
Ultrafast time-resolved wide angle x-ray scattering from chemical reactions in solution has recently emerged as a powerful technique for determining the structural dynamics of transient photochemical species. Here we examine evolution photoexcited CH2I2 nonpolar solvent cyclohexane and draw comparisons with similar study polar methanol. As earlier spectroscopic studies, our data confirm common initial reaction pathway both solvents. After photoexcitation, dissociates to form CH2I⋅+I⋅. Iodine...
The consequences of a recently proposed model [A. Rydh, \"O. Rapp, and M. Andersson, Phys. Rev. Lett. $83,$ 1850 (1999)] for the vortex-liquid resistivity close to vortex liquid-to-glass transition are analyzed in detail. We find detailed quantitative agreement between measurements on disordered, optimally doped ${\mathrm{YBa}}_{2}{\mathrm{Cu}}_{3}{\mathrm{O}}_{7\ensuremath{-}\ensuremath{\delta}}$ single crystals. For temperatures below superconducting temperature, scaling all measured...