A5045105052- Cellular transport and secretion
- Lipid Membrane Structure and Behavior
- Cellular Mechanics and Interactions
- Retinal Development and Disorders
- Microtubule and mitosis dynamics
- Pancreatic function and diabetes
- Erythrocyte Function and Pathophysiology
- Glycosylation and Glycoproteins Research
- Extracellular vesicles in disease
- Protease and Inhibitor Mechanisms
- Lysosomal Storage Disorders Research
- Autophagy in Disease and Therapy
- Nuclear Structure and Function
- CRISPR and Genetic Engineering
- RNA Interference and Gene Delivery
- Proteoglycans and glycosaminoglycans research
- Calcium signaling and nucleotide metabolism
- DNA Repair Mechanisms
University of Oslo
2012-2024
Oslo University Hospital
2019-2024
Overexpression of the transmembrane matrix metalloproteinase MT1-MMP/MMP14 promotes cancer cell invasion. Here we show that MT1-MMP-positive cells turn MT1-MMP-negative invasive by transferring a soluble catalytic ectodomain MT1-MMP. Surprisingly, this effect depends on presence TKS4 and TKS5 in donor cell, adaptor proteins previously implicated invadopodia formation. In endosomes TKS4/5 promote ADAM-mediated cleavage MT1-MMP bridging two proteases, is stimulated low intraluminal pH...
Rab GTPases are key regulators of intracellular trafficking, and cycle between a GTP-bound active state GDP-bound inactive state. This is regulated by guanine-nucleotide exchange factors (GEFs) GTPase-activating proteins (GAPs). Several efforts have been made in connecting the correct GEFs GAPs to their specific Rab. Here, we aimed identify for Rab7b, small GTPase involved transport from late endosomes trans-Golgi. An siRNA screen targeting containing TBC domains critical was performed...
Scientific Report23 August 2017Open Access Transparent process Rab7b modulates autophagic flux by interacting with Atg4B Ingrid Kjos Department of Biosciences, University Oslo, Norway Centre for Immune Regulation, Search more papers this author Marita Borg Distefano Frank Sætre Molecular Medicine Norway, Urska Repnik Petter Holland Institute Basic Medical Sciences, Arwyn T Jones Cardiff School Pharmacy and Pharmaceutical University, Wales, UK Nikolai Engedal Anne Simonsen Oddmund Bakke...
Significance Intraluminal vesicle (ILV) formation plays a crucial role in the attenuation of growth factor receptor signaling. The endosomal sorting complex required for transport (ESCRT-0 to -III/VPS4) mediates this process. general dogma has been that upstream ESCRTs (0 II) sequester receptors at surface endosomes and downstream (III/VPS4) remodel endosome membrane leading abscission receptor-containing ILVs. We now show not only cargo, but addition play initiation shape remodeling ILV...
The members of the Rab family small GTPases are molecular switches that regulate distinct steps in different membrane traffic pathways. In addition to this canonical function, Rabs can play a role other processes, such as cell adhesion and motility. Here, we reveal GTPase Rab18 positive regulator directional migration chemotaxis, underlying mechanism. We show knockdown reduces size focal adhesions (FAs) influences their dynamics. Furthermore, found Rab18, by directly interacting with...
Rab proteins are small GTPases essential for controlling and coordinating intracellular traffic. The GTPase Rab7b regulates the retrograde transport from late endosomes toward Trans-Golgi Network (TGN), is important proper trafficking of several receptors such as Toll-like (TLRs) sorting receptors. We recently identified actin motor protein myosin II a new interaction partner Rab7b, found that dependent on II. Interestingly, we also discovered influences phosphorylation state by activation...
Proteoglycan (PG) sulfation depends on activated nucleotide sulfate, 3′-phosphoadenosine-5′-phosphosulfate (PAPS). Transporters in the Golgi membrane translocate PAPS from cytoplasm into organelle lumen where PG occurs. Silencing of transporter (PAPST) 1 epithelial MDCK cells reduced uptake vesicles. Surprisingly, at same time heparan sulfate (HS) was stimulated. The effect pathway specific polarized cells. Basolaterally secreted proteoglycans (PGs) displayed an altered HS pattern and...
Rab proteins are well known regulators of intracellular trafficking; however, more and studies point to their function also in other cellular processes, including cell migration. In this work, we have performed an siRNA screen identify that influence The revealed Rab33b as the strongest candidate affected motility. has been previously reported localize at Golgi apparatus regulate Golgi-to-ER retrograde trafficking homeostasis. We mediates post-Golgi transport plasma membrane. further...
Abstract As part of the lysosomal degradation pathway, endosomal sorting complexes required for transport (ESCRT-0-III/VPS4) sequester receptors at endosome and simultaneously deform membrane to generate intraluminal vesicles (ILVs). Whereas ESCRT-III/VPS4 have an established function in ILV formation, role upstream ESCRTs (0-II) shape remodeling is not understood. Combining experimental measurements electron microscopy analysis ESCRT-III depleted cells with a mathematical model, we show...