A5069550997- Monoclonal and Polyclonal Antibodies Research
- Venomous Animal Envenomation and Studies
- Toxin Mechanisms and Immunotoxins
- Rabies epidemiology and control
- Protein purification and stability
- Nanofabrication and Lithography Techniques
- vaccines and immunoinformatics approaches
- Insect and Pesticide Research
- Cell Adhesion Molecules Research
- Healthcare and Venom Research
- Glycosylation and Glycoproteins Research
- SARS-CoV-2 and COVID-19 Research
- Biochemical and Structural Characterization
- Ion channel regulation and function
- Forensic Toxicology and Drug Analysis
- Rheumatoid Arthritis Research and Therapies
- Radiopharmaceutical Chemistry and Applications
- Peptidase Inhibition and Analysis
- Studies on Chitinases and Chitosanases
- Fibroblast Growth Factor Research
- Marine Toxins and Detection Methods
- Advanced Biosensing Techniques and Applications
- Protease and Inhibitor Mechanisms
- Nicotinic Acetylcholine Receptors Study
- Botulinum Toxin and Related Neurological Disorders
Cambridgeshire and Peterborough NHS Foundation Trust
2021-2022
Technical University of Denmark
2020
Universidad de Costa Rica
2020
Novo Nordisk Foundation
2020
Universidade de São Paulo
2020
Eskişehir Osmangazi University
2020
Universidade Federal de Roraima
2020
Babraham Institute
2017
University of Cambridge
2010
The black mamba (Dendroaspis polylepis) is one of the most feared snake species African savanna. It has a potent, fast-acting neurotoxic venom comprised dendrotoxins and α-neurotoxins associated with high fatality in untreated victims. Current antivenoms are both scarce on continent present number drawbacks as they derived from plasma hyper-immunized large mammals. Here, we describe development an experimental recombinant antivenom by combined toxicovenomics phage display approach. based...
Abstract Snakebite envenoming continues to claim many lives across the globe, necessitating development of improved therapies. To this end, broadly-neutralizing human monoclonal antibodies may possess advantages over current plasma-derived antivenoms by offering superior safety and high neutralization capacity. Here, we report establishment a pipeline based on phage display technology for discovery optimization affinity antibodies. This approach yielded recombinant antibody with capacities...
Abstract Improved therapies are needed against snakebite envenoming, which kills and permanently disables thousands of people each year. Recently developed neutralizing monoclonal antibodies several snake toxins have shown promise in preclinical rodent models. Here, we use phage display technology to discover a human antibody show that this causes antibody-dependent enhancement toxicity (ADET) myotoxin II from the venomous pit viper, Bothrops asper , mouse model envenoming mimics snakebite....
The monocled cobra (Naja kaouthia) is among the most feared snakes in Southeast Asia due to its toxicity, which predominantly derived from long-chain α-neurotoxins. only specific treatment for snakebite envenoming antivenom based on animal-derived polyclonal antibodies. Despite lifesaving importance of these medicines, major limitations safety, supply consistency, and efficacy create a need improved treatments. Here, we describe discovery subsequent optimization recombinant human monoclonal...
To demonstrate the utility of phage display in generating highly specific antibodies, affinity selections were conducted on 20 related Src Homology 2 (SH2) domains (ABL1, ABL2, BTK, BCAR3, CRK, FYN, GRB2, GRAP2, LYN, LCK, NCK1, PTPN11 C, PIK3R1 PLCγ1 RASA1 SHC1, SH2D1A, SYK N, VAV1 and tandem ZAP70). The expressed Escherichia coli, purified used selection experiments. In total, 1292/3800 resultant antibodies shown to bind target antigen. Of 695 further evaluated specificity ELISAs against...
// Kenneth A. Botkjaer 1 , Hang Fai Kwok 1, 8 Mikkel G. Terp 2 Aneesh Karatt-Vellatt 3 Salvatore Santamaria 4 John McCafferty Peter Andreasen 5, 6 Yoshifumi Itoh Henrik J. Ditzel 2, 7 Gillian Murphy Department of Oncology, University Cambridge, Cancer Research UK Cambridge Institute, Li Ka Shing Centre, CB2 0RE, U.K and Inflammation Research, Institute Molecular Medicine, Southern Denmark, Odense, Denmark IONTAS Ltd., Babraham Campus, CB22 3AT, Kennedy Rheumatology, Oxford, Headington,...
Passive immunization using monoclonal antibodies will play a vital role in the fight against COVID-19. The recent emergence of viral variants with reduced sensitivity to some current and vaccines highlights importance broad cross-reactivity. This study describes deep-mining antibody repertoires hospitalized COVID-19 patients phage display technology B cell receptor (BCR) repertoire sequencing isolate neutralizing gain insights into early response. comprehensive discovery approach has yielded...
Objectives Controlled immune responses rely on integrated crosstalk between cells and their microenvironment. We investigated whether targeting proinflammatory signals from the extracellular matrix that persist during pathological inflammation provides a viable strategy to treat rheumatoid arthritis (RA). Methods Monoclonal antibodies recognising fibrinogen-like globe (FBG) of tenascin-C were generated by phage display. Clones neutralised FBG activation toll-like receptor 4 (TLR4), without...
Abstract The growth and motility factor Hepatocyte Growth Factor/Scatter Factor (HGF/SF) its receptor, the product of MET proto-oncogene, promote invasion metastasis tumor cells have been considered potential targets for cancer therapy. We generated a new Met-blocking antibody which binds outside ligand-binding site, determined crystal structure Fab in complex with target, identifies binding site as Met Ig1 domain. antibody, 107_A07, inhibited HGF/SF-induced cell migration proliferation...
Abstract The monocled cobra ( Naja kaouthia ) is one of the most feared snakes in Southeast Asia. It a highly dangerous species with potent venom deriving its toxicity predominantly from abundant long-chain α-neurotoxins. only specific treatment for snakebite envenoming antivenom, which based on animal-derived polyclonal antibodies. Despite lifesaving importance these medicines over past 120 years, and their ongoing role combating disease, major limitations safety, supply consistency,...
Abstract Passive immunisation using monoclonal antibodies will play a vital role in the fight against COVID-19. Until now, majority of anti-SARS-CoV-2 antibody discovery efforts have relied on screening B cells patients convalescent phase. Here, we describe deep-mining repertoires hospitalised COVID-19 combination phage display technology and cell receptor (BCR) repertoire sequencing to isolate neutralising gain insights into early response. This comprehensive approach has yielded potent...
Abstract Snakebite envenoming continues to claim many lives across the globe, necessitating development of improved therapies. To this end, human monoclonal antibodies may possess advantages over current plasma-derived antivenoms by offering superior safety and neutralization capacity. However, as new antivenom products need be polyvalent, i.e ., target multiple different snake species, without dramatically increasing dose or cost manufacture, such broadly-neutralizing. Here, we report...