A5005403171- Intensive Care Unit Cognitive Disorders
- Monoclonal and Polyclonal Antibodies Research
- Anesthesia and Sedative Agents
- Computational Drug Discovery Methods
- Anesthesia and Neurotoxicity Research
- Respiratory Support and Mechanisms
- Enzyme Structure and Function
- SARS-CoV-2 and COVID-19 Research
- Protein purification and stability
- Family and Patient Care in Intensive Care Units
- Cardiac Arrest and Resuscitation
- vaccines and immunoinformatics approaches
- Immune Cell Function and Interaction
- Fibromyalgia and Chronic Fatigue Syndrome Research
- NF-κB Signaling Pathways
- Analytical chemistry methods development
- Diverse Education Studies and Reforms
- HER2/EGFR in Cancer Research
- Ultrasound in Clinical Applications
- Ophthalmology and Visual Health Research
- Cancer Immunotherapy and Biomarkers
- Healthcare Policy and Management
- COVID-19 and Mental Health
- Ubiquitin and proteasome pathways
- Alzheimer's disease research and treatments
Glasgow Royal Infirmary
2020-2024
Queen Elizabeth University Hospital
2024
NHS Greater Glasgow and Clyde
2024
University of Leicester
2016-2023
Institute of Structural and Molecular Biology
2021-2022
Royal Alexandra Hospital
2018
University of the West of Scotland
2018
University of Warwick
2008
Armed Forces Hospital Southern Region
1991
Nottinghamshire County Council
1991
Passive immunization using monoclonal antibodies will play a vital role in the fight against COVID-19. The recent emergence of viral variants with reduced sensitivity to some current and vaccines highlights importance broad cross-reactivity. This study describes deep-mining antibody repertoires hospitalized COVID-19 patients phage display technology B cell receptor (BCR) repertoire sequencing isolate neutralizing gain insights into early response. comprehensive discovery approach has yielded...
In Intensive Care Units (ICUs), patients are at risk of developing ocular complications, especially exposure keratopathy. Plan, Do, Study, Act for PDSA cycle. Despite national guidelines, implementation remains challenging. Using the cycle, we devised an eye care protocol integrated into electronic patient record system, complemented by a poster summary guidelines. An initial audit showed 2% adherence to guidelines; post-intervention, rose 76%. A 9-month analysis revealed 16% experienced in...
A critically ill infant with paroxysmal supraventricular tachycardia and hydrops fetalis responded well to aggressive management. Care must be taken avoid digitalis toxicity. Procaine amide or quinidine are effective alternate therapies.
Significance The protease MALT1 has been shown to play an essential role in the adaptive immune response and development of lymphoma. catalytic activity is tightly regulated by monoubiquitination, however, how ubiquitin conjugated manner which this modification regulates remains poorly understood. Our data suggest that Ig3 domain physically recruits enable monoubiquitination conjugation activates inducing conformational changes are communicated active site. These findings identify as a novel...
Here we introduce a new concept for synthesising molecularly imprinted nanoparticles by using proteins as macro-functional monomers. For proof-of-concept, model enzyme (HRP) was cross-linked glutaraldehyde in the presence of glass beads (solid-phase) bearing immobilized templates such vancomycin and ampicillin. The cross-linking process links together protein chains, which leads to formation permanent target-specific recognition sites without adverse effects on enzymatic activity. Unlike...
Programmed death-ligand 1 (PD-L1) is a key immune regulatory protein that interacts with programmed cell death (PD-1), leading to T-cell suppression. Whilst this interaction in self-tolerance, cancer cells evade the system by overexpressing PD-L1. Inhibition of PD-1/PD-L1 pathway standard monoclonal antibodies has proven highly effective treatment; however, single domain (VHH) may offer numerous potential benefits. Here, we report identification and characterization diverse panel 16 novel...
Interleukin-16 (IL-16) is reported to be a chemoattractant cytokine and modulator of T-cell activation, has been proposed as ligand for the co-receptor CD4. The secreted active form IL-16 detected at sites TH1-mediated inflammation, such those seen in autoimmune diseases, ischemic reperfusion injury (IRI), tissue transplant rejection. Neutralization recruitment its receptor, using an anti-IL16 antibody, shown significantly attenuate inflammation disease pathology IRI, well some diseases....
Plexin-B1 is a receptor for the cell surface semaphorin, Sema4D. This signaling system has been implicated in variety of human diseases, including cancer, multiple sclerosis and osteoporosis. While inhibitors Plexin-B1:Sema4D interaction have previously reported, understanding their mechanism hindered by an incomplete structural view Plexin-B1. In this study, we raised characterized pair nanobodies that are specific mouse which inhibit binding Sema4D to its biological activity. Structural...
Abstract Background In-hospital cardiac arrest/periarrest is a recognised trigger for consideration of admission to the intensive care unit (ICU). We aimed investigate rates ICU following in-hospital arrest/periarrest, evaluate outcomes such patients and assess whether anticipatory planning had taken place prior adult resuscitation team being called. Methods Analysis all referrals page-holder within our district general hospital between 1st November 2018 31st May 2019. From this, frequency...
Abstract Passive immunisation using monoclonal antibodies will play a vital role in the fight against COVID-19. Until now, majority of anti-SARS-CoV-2 antibody discovery efforts have relied on screening B cells patients convalescent phase. Here, we describe deep-mining repertoires hospitalised COVID-19 combination phage display technology and cell receptor (BCR) repertoire sequencing to isolate neutralising gain insights into early response. This comprehensive approach has yielded potent...
The post-transcriptional modifier tRNA-(N1G37) methyltransferase (TrmD) has been proposed to be essential for growth in many Gram-negative and Gram-positive pathogens, however previously reported inhibitors show only weak antibacterial activity. In this work, optimisation of fragment hits resulted compounds with low nanomolar TrmD inhibition incorporating features designed enhance bacterial permeability covering a range physicochemical space. resulting lack significant activity suggests that...
Human epidermal growth factor receptor-2 (HER2) is a well-recognised biomarker associated with 25% of breast cancers. In most cases, early detection and/or treatment correlates an increased chance survival. This study, has identified and characterised highly specific anti-HER2 single-domain antibody (sdAb), NM-02, as potential theranostic tool. Complete structural description by X-ray crystallography revealed non-overlapping epitope current antibodies. To reduce the immunogenicity risk,...
Summary A 51‐year‐old male required invasive ventilation following smoke inhalation in a house fire. During his intensive care admission, he developed massive haemoptysis and subsequent life‐threatening respiratory failure likely due to diffuse alveolar haemorrhage. We discuss the management of pulmonary haemorrhage critically hypoxic patient requiring prone positioning bronchoscopy simultaneously.
for our patients in renal failure should not be greater than concern other human beings, even if we are nephrologists or transplant surgeons.Giving a fair price is the solution poverty.This worldwide problem.Opening such door will add to miseries ofThird World countries as they would become sole source of organs sale.