A5076988340- Mass Spectrometry Techniques and Applications
- Venomous Animal Envenomation and Studies
- Rabies epidemiology and control
- Analytical Chemistry and Chromatography
- Electrochemical sensors and biosensors
- Parkinson's Disease Mechanisms and Treatments
- Diabetes and associated disorders
- Alzheimer's disease research and treatments
- Plasma Applications and Diagnostics
- Toxin Mechanisms and Immunotoxins
- Mosquito-borne diseases and control
- Hemoglobin structure and function
- Neurological disorders and treatments
- Biochemical and Structural Characterization
- Diabetes Management and Research
- Insect and Pesticide Research
- Isotope Analysis in Ecology
- Radioactive element chemistry and processing
- Hyperglycemia and glycemic control in critically ill and hospitalized patients
- Metabolomics and Mass Spectrometry Studies
- Burn Injury Management and Outcomes
- Glycosylation and Glycoproteins Research
- Adipokines, Inflammation, and Metabolic Diseases
- COVID-19 Clinical Research Studies
- Microfluidic and Capillary Electrophoresis Applications
ETH Zurich
2021-2024
National Technical University "Kharkiv Polytechnic Institute"
2024
York University
2018-2021
Abstract Snakebite envenoming continues to claim many lives across the globe, necessitating development of improved therapies. To this end, broadly-neutralizing human monoclonal antibodies may possess advantages over current plasma-derived antivenoms by offering superior safety and high neutralization capacity. Here, we report establishment a pipeline based on phage display technology for discovery optimization affinity antibodies. This approach yielded recombinant antibody with capacities...
Structural isomers of N-glycans that are identical in mass and atomic composition provide a great challenge to conventional spectrometry (MS). This study employs additional dimensions structural elucidation including ion mobility (IM) spectroscopy coupled hydrogen/deuterium exchange (HDX) electron capture dissociation (ECD) characterize three main A2 their conformers. A series IM–MS experiments were able separate the low abundance linkage-based (α1-3 α1-6 for A2G1). HDX–IM–MS data indicated...
Abstract Plasma medicine is a field that utilizes reactive species generated from atmospheric low-temperature plasmas for applications such as sterilization, blood coagulation, and cancer therapy. Commercial plasma devices are available wound healing, but research on the chemical modifications induced by these scarce. This study explores in hemoglobin when exposed to helium dielectric barrier discharge, with aim of explaining potential mechanisms through which it contributes coagulation...
Brazil is home to a multitude of venomous snakes; perhaps the most medically relevant which belong Bothrops genus. spp. are responsible for roughly 70% all snakebites in Brazil, and envenomings caused by their bites can be treated with three types antivenom: bothropic antivenom, bothro-lachetic bothro-crotalic antivenom. The choice administer antivenom depends on severity envenoming, while availability how certain treating physician that patient was bitten snake. diagnosis envenoming made...
Both normal and pathological functions of α-synuclein (αSN), an abundant protein in the central peripheral nervous system, have been linked to its interaction with membrane lipid bilayers. The ability characterize structural transitions αSN upon complexation will clarify molecular mechanisms associated αSN-linked pathologies, including Parkinson's disease (PD), multiple systems atrophy, other synucleinopathies. In this work, time-resolved electrospray ionization hydrogen/deuterium exchange...
Abstract Low-temperature plasma (LTP) jets are Food and Drug Administration (FDA)-approved medical devices to remove cancerous tissue aid in wound healing. However, reports on their reaction with proteins conflicting, ranging from fragmentation, oxidation, aggregation, or a combination thereof. In this study we bridge the gap between plasma-treatment of short peptides at physiologically relevant concentrations. The LTP is based helium dielectric barrier discharge that forms plasma-jet, which...
Investigating snake venom is necessary for developing new treatments envenoming and harnessing the therapeutic potential that lies within toxins. Despite considerable efforts in previous research, several technical challenges remain characterizing individual components such complex mixtures. Here, we present native top-down mass spectrometry (MS) workflows enable analysis of proteins mixtures showcase utility these methodologies on King cobra (
It has been established that the most severe course of COVID-19 is observed in individuals with chronic diseases, including those diabetes mellitus.There are reports literature cases hyperglycemia first registered against background coronavirus infection.Aims.To determine frequency previously diagnosed and newly mellitus patients infected COVID-19.To assess relationship between level glycated hemoglobin (HbA1c) markers inflammatory process severity infectious disease.Methods.Data collection...
Abstract Snakebite envenoming continues to claim many lives across the globe, necessitating development of improved therapies. To this end, human monoclonal antibodies may possess advantages over current plasma-derived antivenoms by offering superior safety and neutralization capacity. However, as new antivenom products need be polyvalent, i.e ., target multiple different snake species, without dramatically increasing dose or cost manufacture, such broadly-neutralizing. Here, we report...
Abstract Both normal and pathological functions of α-synuclein (αSN), an abundant protein in the central peripheral nervous system, have been linked to its interaction with membrane lipid bilayers. The ability characterize structural transitions αSN upon complexation will clarify molecular mechanisms associated αSN-linked pathologies, including Parkinson’s disease (PD), Multiple Systems Atrophy other synucleinopathies. In this work, Time-Resolved ElectroSpray Ionization Hydrogen/ Deuterium...
Abstract Background Brazil is home to a multitude of venomous snakes, perhaps the most medically relevant which belong Bothrops genus. spp . are responsible for roughly 70% all snakebites in Brazil, and envenomings caused by their bites can be treated with three types antivenom: bothropic antivenom, bothro-lachetic bothro-crotalic antivenom. The choice antivenom that administered depends not only on its availability how certain treating physician patient was bitten snake. diagnosis...