A5039779455- Biosensors and Analytical Detection
- Advanced Biosensing Techniques and Applications
- Venomous Animal Envenomation and Studies
- Food Allergy and Anaphylaxis Research
- SARS-CoV-2 detection and testing
- Biomedical and Engineering Education
- Rabies epidemiology and control
- Mosquito-borne diseases and control
- Advanced biosensing and bioanalysis techniques
- Microfluidic and Bio-sensing Technologies
- Plant Virus Research Studies
- Plant and animal studies
- Monoclonal and Polyclonal Antibodies Research
- Pesticide Residue Analysis and Safety
- Listeria monocytogenes in Food Safety
- Contact Dermatitis and Allergies
- Scientific Computing and Data Management
- Glycosylation and Glycoproteins Research
- Plant and Fungal Interactions Research
- Plant Pathogens and Fungal Diseases
- Mycotoxins in Agriculture and Food
- Ecology and Vegetation Dynamics Studies
- Wheat and Barley Genetics and Pathology
- Animal Vocal Communication and Behavior
- Health, Environment, Cognitive Aging
Wageningen University & Research
2018-2023
University of Bristol
2010-2013
Sandwich lateral flow immunoassays (LFIAs) are limited at high antigen concentrations by the hook effect, leading to a contradictory decrease in test line (T) intensity and false-negative results. The effect is mainly associated with loss of T, research focuses on minimizing this effect. Nevertheless, control (C) also affected higher analyte concentrations, undesirably influencing T/C ratio LFIA readers. main aim work identify understand these concentration effects order develop ubiquitous...
Food contaminants monitoring is conducted in an intensive manner yet, there are still food safety scandals related to various chemical compounds. This fact highlights the need review requirements posed by current legal framework on analytical methods performance and evaluate its application published studies. Herein, we present inventory including more than 470 publications screening confirmatory methods, which were used control hazardous compounds such as pesticides, antibiotics,...
Seed dispersal is a central process in plant ecology with consequences for species composition and habitat structure. Some bird are known to disperse the seeds they ingest, whereas others, termed ‘seed predators’, digest them apparently play no part dispersal, but it not clear if these discrete strategies or simply ends of continuum. We assessed effectiveness by combining analysis faecal samples density. The droppings seed dispersers contained more entire than those typical predators, over...
(1) Background: The lack of globally standardized allergen labeling legislation necessitates consumer-focused multiplexed testing devices. These should be easy to operate, fast, sensitive and robust. (2) Methods: Herein, we describe the development three different formats for food detection, namely active passive flow-through assays, lateral flow immunoassays with test line configurations. (3) Results: fastest assay time was 1 min, whereas even slowest within 10 min. With approach, limits...
Food allergies are hypersensitivity immune responses triggered by (traces of) allergenic compounds in foods and drinks. The recent trend towards plant-based lactose-free diets has driven an increased consumption of milks (PBMs) with the risk cross-contamination various proteins during food manufacturing process. Conventional allergen screening is usually performed laboratory, but portable biosensors for on-site allergens at production site could improve quality control safety. Here, we...
Bothrops and Lachesis are two of Brazil's medically most relevant snake genera, causing tens thousands bites annually. Fortunately, Brazil has good accessibility to high-quality antivenoms at the genus inter-genus level, enabling treatment many these envenomings. However, optimal use treatments requires that species responsible for bite is determined. Currently, physicians a syndromic approach diagnose snakebite, which can be difficult medical personnel with limited training in clinical...
Smartphones are ubiquitous in modern society; 2021, the number of active subscriptions surpassed 6 billion. These devices have become more than a means communication; smartphones powerful, continuously connected, miniaturized computers capable passively and actively collecting (private) information for us from us. Their implementation as detectors or instrumental interfaces emerging smartphone-based (bio)sensors (SbSs) has facilitated shift towards portable point-of-care platforms healthcare...
Paper-based analytical devices (PADs) enable the affordable, easy, rapid, and reliable detection of a range analytes at point-of-care. Cellulose is versatile substrate for antibody attachment. However, how immunoreagents are immobilized onto cellulose plays an important role in assay performance. To provide overview different immobilization strategies used developing PADs, we here critically review existing literature from last decade (2013-2023). First, introduce as summarize fabrication...
Lateral Flow Immunoassays (LFIAs) allow for rapid, low-cost, screening of many biomolecules such as food allergens. Despite being classified rapid tests, LFIAs take 10–20 min to complete. For a really high-speed LFIA, it is necessary assess antibody association kinetics. By using label-free optical technique Surface Plasmon Resonance (SPR), possible screen crude monoclonal (mAb) preparations their rates against target. Herein, we describe an SPR-based method and selecting anti-hazelnut...
The amalgamation of computer-like capabilities and portability modern smartphones has fuelled their implementation as detectors interfaces in emerging smartphone-based (bio)sensors (SbSs) for e.g. healthcare, point-of-need, food safety, environmental science, forensics systems. SbSs intrinsically carry great potential consumer diagnostics, future ‘citizen science’ approaches, which have far-reaching implications the technological, legal, ethical aspects associated with research, development,...
Brazil is home to a multitude of venomous snakes; perhaps the most medically relevant which belong Bothrops genus. spp. are responsible for roughly 70% all snakebites in Brazil, and envenomings caused by their bites can be treated with three types antivenom: bothropic antivenom, bothro-lachetic bothro-crotalic antivenom. The choice administer antivenom depends on severity envenoming, while availability how certain treating physician that patient was bitten snake. diagnosis envenoming made...
Highlights•IgGs against the snake venom toxins α-cobratoxin and myotoxin II were discovered•Light-chain shuffling was used to generate IgGs with pH-dependent binding properties•Binding between IgG affects FcRn-mediated cellular handling•Cellular handing of IgG-bound is complex affected by multiple factorsSummaryImmunoglobulin G (IgG) antibodies that bind their cognate antigen in a manner (acid-switched antibodies) can release bound for degradation acidic environment endosomes, while are...
The increasing demand for food and feed products is stretching the capacity of value chain to its limits. A key step ensuring safety checking mycotoxin contamination wheat. However, this analysis typically performed by rather complex expensive chromatographic methods, such as liquid chromatography-tandem mass spectrometry (LC-MS/MS). These costly methods require extensive sample preparation that not easily carried out at different points along supply chain. To overcome challenges in...
Abstract Background Brazil is home to a multitude of venomous snakes, perhaps the most medically relevant which belong Bothrops genus. spp . are responsible for roughly 70% all snakebites in Brazil, and envenomings caused by their bites can be treated with three types antivenom: bothropic antivenom, bothro-lachetic bothro-crotalic antivenom. The choice antivenom that administered depends not only on its availability how certain treating physician patient was bitten snake. diagnosis...