A5079359471- Biosensors and Analytical Detection
- Advanced Biosensing Techniques and Applications
- Advanced biosensing and bioanalysis techniques
- Identification and Quantification in Food
- Bacteriophages and microbial interactions
- Protein Hydrolysis and Bioactive Peptides
- Nanofabrication and Lithography Techniques
- Monoclonal and Polyclonal Antibodies Research
- Pharmacological Effects and Assays
- Peroxisome Proliferator-Activated Receptors
- Escherichia coli research studies
- Drug Transport and Resistance Mechanisms
- Sesquiterpenes and Asteraceae Studies
- Mosquito-borne diseases and control
- Malaria Research and Control
- Herbal Medicine Research Studies
- Cholesterol and Lipid Metabolism
- Advanced Chemical Sensor Technologies
- Biochemical and Structural Characterization
- Innovative Microfluidic and Catalytic Techniques Innovation
- Antibiotics Pharmacokinetics and Efficacy
- Plant Toxicity and Pharmacological Properties
- Meat and Animal Product Quality
- Antimicrobial Peptides and Activities
- Pesticide Residue Analysis and Safety
Wageningen University & Research
2013-2025
University of Twente
2008
Academic Center for Dentistry Amsterdam
2006
Universitat Politècnica de València
2005
Universidad Complutense de Madrid
2005
Utrecht University
1982-1996
Freie Universität Berlin
1996
Erasmus University Rotterdam
1988
ABSTRACT A newly developed reagent strip assay for the diagnosis of schistosomiasis based on parasite antigen detection in urine infected individuals was evaluated. The test uses principle lateral flow through a nitrocellulose sample mixed with colloidal carbon conjugate monoclonal antibody specific Schistosoma circulating cathodic (CCA). to diagnose group highly schoolchildren Mwanza, Tanzania, demonstrated high sensitivity and association intensity infection as measured both by egg counts,...
The use of carbon nanoparticles is shown for the detection and identification different Shiga toxin-producing Escherichia coli virulence factors (vt1, vt2, eae ehxA) a 16S control (specific E. coli) based on lateral flow strips (nucleic acid immunoassay, NALFIA). Prior to with NALFIA, rapid amplification method tagged primers was applied. In evaluation optimised NALFIA strips, no cross-reactivity found any antibodies used. limit higher than quantitative PCR (q-PCR), in most cases between...
A broad-specific ELISA using one antibody preparation for the detection of gentamicin, kanamycin, and neomycin in milk is reported first time. For immunization rabbits, neamin was used as generic hapten on basis facts that it a two-ring fragment and, shape charge, resembles parts kanamycin gentamicin. Neamin linked to preactivated carrier protein keyhole limpet hemocyanin by EDC coupling. The specificity sensitivity polyclonal antibodies aminoglycoside antibiotics were tested competitive...
We present a new nucleic acid lateral flow immunoassay (NALFIA) for the assessment of listeria contamination. The detection procedure starts with enrichment sample in Half Fraser broth (24 h). Following isolation DNA, duplex PCR is performed two labelled primer sets, one generic and directed to specific sequence gene encoding 16S rRNA from Listeria spp. other part prfA central virulence regulator food pathogen monocytogenes (3.5 solution directly added one-step assay device appearance...
The assembly of complex bionanostructures onto β-cyclodextrin (βCD) monolayers has been investigated with the aims antibody recognition and cell adhesion. formation these assemblies relies on host−guest, protein−ligand, protein−protein interactions. buildup a structure consisting divalent bis(adamantyl)-biotin linker, streptavidin (SAv), biotinylated protein A (bt-PA), an Fc fragment human immunoglobin G (IgG-Fc) was studied surface plasmon resonance (SPR) spectroscopy. Patterns this...
Declining malaria transmission and known difficulties with current diagnostic tools for malaria, such as microscopy rapid tests (RDTs) in particular at low parasite densities, still warrant the search sensitive tests. Molecular need substantial simplification before implementation clinical settings countries where is endemic. Direct blood PCR (db-PCR), circumventing DNA extraction, to detect Plasmodium was developed adapted be visualized by nucleic acid lateral flow immunoassay (NALFIA). The...
Background Dipeptidyl peptidase 4 (DPP4) and angiotensin-converting enzyme (ACE) are important target enzymes in glycemic control renovascular protection. Here, we studied the effect of NWT-03, an egg protein hydrolysate with DPP4- ACE-inhibitory activity, on damage Zucker diabetic fatty (ZDF) rats. Comparisons were made to rats treated vildagliptin (VIL), included as a positive for DPP4 inhibition. Methods ZDF received NWT-03 (1 g/kg/day) or VIL (3 mg/kg/day) from 10 25 weeks age. Metabolic...
An automated immunosensor for the rapid and sensitive analysis of penicillin type β-lactam antibiotics has been developed optimized. immunogen was prepared by coupling common structure penicillanic antibiotics, i.e., 6-aminopenicillanic acid to keyhole limpet hemocyanin. Polyclonal antibodies raised in rabbits after immunization with this conjugate have applied development a competitive fluoroimmunoassay (FIA), using novel fluorescent...
Several aspects of the development competitive lateral flow immunoassays (LFIAs) are described. The quantitation progesterone is taken as an example. LFIA format consisted a nitrocellulose membrane spotted with various conjugates test line. A mixture primary antibody and secondary adsorbed to colloidal carbon was used for signal generation. digital scanner dedicated software were quantitate response. reappraisal checkerboard titration, often in optimisation immunoassays, discussed....
Lateral Flow Immunoassays (LFIAs) allow for rapid, low-cost, screening of many biomolecules such as food allergens. Despite being classified rapid tests, LFIAs take 10–20 min to complete. For a really high-speed LFIA, it is necessary assess antibody association kinetics. By using label-free optical technique Surface Plasmon Resonance (SPR), possible screen crude monoclonal (mAb) preparations their rates against target. Herein, we describe an SPR-based method and selecting anti-hazelnut...
Abstract In this study we explore the potential of using Fourier-transform infrared (FTIR) spectra trifluoroacetate-protein and peptide complexes for monitoring proteolytic reactions. The idea treating dry-films protein hydrolysates with trifluoroacetic acid (TFA) prior to FTIR analysis is based on unique properties TFA. By adding a large excess TFA hydrolysate samples, possible protonation sites proteins peptides will be saturated. addition, has low boiling point when protonated as well...
Pesticides are used in agriculture to prevent pests. Chlorpyrifos (CHLP) is an insecticide with potentially detrimental effects on humans, bees, and the aquatic environment. Its have led a total ban by European Union (EU), but outside EU, CHLP still produced used. An indirect lateral flow immunoassay (LFIA) for detection of was developed integrated into cassette create device (LFD). Species-specific reporter antibodies were coupled carbon nanoparticles detector conjugate. Water samples mixed...