A5055316339- Mycotoxins in Agriculture and Food
- Insect and Pesticide Research
- Advanced biosensing and bioanalysis techniques
- Pesticide Residue Analysis and Safety
- Plant Pathogens and Resistance
- Wheat and Barley Genetics and Pathology
- Plant and animal studies
- Plant tissue culture and regeneration
- Insect and Arachnid Ecology and Behavior
- Indoor Air Quality and Microbial Exposure
- Plant-Microbe Interactions and Immunity
- Plant Pathogenic Bacteria Studies
- Biosensors and Analytical Detection
- Plant Pathogens and Fungal Diseases
- Calibration and Measurement Techniques
- Viral Infectious Diseases and Gene Expression in Insects
- Plant Reproductive Biology
- Bacteriophages and microbial interactions
- Plasmonic and Surface Plasmon Research
- Plant Molecular Biology Research
- Insect Resistance and Genetics
- Plant and fungal interactions
- Advanced Biosensing Techniques and Applications
- Plant Disease Resistance and Genetics
- Plant Virus Research Studies
Wageningen University & Research
2011-2024
Airbus (Netherlands)
2023
Centre for BioSystems Genomics
2002-2007
Graduate School Experimental Plant Sciences
1996
Currently beer is booming, mainly due to the steady rise of craft breweries worldwide. Previous surveys for occurrence mycotoxins in beer, were focussed on industrial produced beer. The present survey reports presence and how this compares More than 1000 beers collected from 47 countries, which 60% beers. A selection samples screened aflatoxin B1, ochratoxin (OTA), zearalenone (ZEN), fumonisins (FBs), T-2 HT-2 toxins (T-2 HT-2) deoxynivalenol (DON) using a mycotoxin 6-plex immunoassay. For...
Optical biosensors based on plasmonic sensing schemes combine high sensitivity and selectivity with label-free detection. However, the use of bulky optical components is still hampering possibility obtaining miniaturized systems required for analysis in real settings. Here, a fully biosensor prototype detection demonstrated, which enables fast multiplex analytes high- low molecular weight (80 000 582 Da) as quality safety parameters milk: protein (lactoferrin) an antibiotic (streptomycin)....
A 6-plex mycotoxin assay was developed on a portable nanostructured iSPR and compared with benchmark double 3-plex SPR assay.
A multi-mycotoxin immunoassay-using the MultiAnalyte Profiling (xMAP) technology-is developed and evaluated. This technology combines a unique color-coded microsphere suspension array, with dedicated flow cytometer. We aimed for combined detection of aflatoxins, ochratoxin A, deoxynivalenol, fumonisins, zearalenone T-2-toxin in an inhibition immunoassay format. Sets six mycotoxin-protein conjugates specific monoclonal antibodies were selected, we observed good sensitivities no...
To study the molecular regulation of nectary development, we cloned NEC1, a gene predominantly expressed in nectaries Petunia hybrida, by using differential display RT-PCR technique. The secondary structure putative NEC1 protein is reminiscent transmembrane protein, indicating that incorporated into cell membrane or cytoplast membrane. Immunolocalization revealed present nectaries. Northern blot analyses showed highly tissue and weakly stamen. GUS expression driven promoter activity outer...
Multi-analyte binding assays for rapid screening of food contaminants require mass spectrometric identification compound(s) in suspect samples. An optimal combination is obtained when the same bioreagents are used both methods; moreover, miniaturisation important because high costs bioreagents. A concept demonstrated using superparamagnetic microbeads coated with monoclonal antibodies (Mabs) a novel direct inhibition flow cytometric immunoassay (FCIA) plus immunoaffinity isolation prior to...
Pesticides are used in agriculture to prevent pests. Chlorpyrifos (CHLP) is an insecticide with potentially detrimental effects on humans, bees, and the aquatic environment. Its have led a total ban by European Union (EU), but outside EU, CHLP still produced used. An indirect lateral flow immunoassay (LFIA) for detection of was developed integrated into cassette create device (LFD). Species-specific reporter antibodies were coupled carbon nanoparticles detector conjugate. Water samples mixed...
A combined (triplex) immunoassay for the simultaneous detection of three mycotoxins in grains was developed with superparamagnetic colour-encoded microbeads, combination two bead-dedicated flow cytometers. Monoclonal antibodies were coupled to beads, and amounts bound inversely related fluorescent labelled (inhibition format). The selected monoclonal tested their target cross-reactivity relevant metabolites masked mycotoxins. In triplex format, low levels cross-interactions between assays...
Mycotoxins are produced by fungi as secondary metabolites. They often multi-contaminate food and feed commodities posing a health risk to humans animals. A fast easy apply multiplex screening of these could be useful detect multi-contamination. For this, we developed semi-quantitative 6-plex immunoassay using suspension array paramagnetic colour-coded microspheres combined with imaging planar detection for the mycotoxins aflatoxin B1, ochratoxin A, zearalenone, deoxynivalenol, T2-toxin, HT-2...
Honey bee colonies have shown abnormal mortality rates over the last decades. Colonies are exposed to biotic and abiotic stressors including landscape changes caused by human pressure. Modern agriculture even forestry, rely on pesticide inputs these chemicals been indicated as one of major causes for colony losses. Neonicotinoids a common class pesticides used worldwide that specific kill insect pests, with acetamiprid being only neonicotinoid allowed be applied outdoors in EU. To evaluate...
An enrichment microsphere immunoassay (MIA) was developed, based on the Luminex xMAP® technology, for simultaneous (duplex) detection of Pectobacterium atrosepticum (former name Erwinia carotovora subsp. atroseptica) (Pca) and Dickeya dianthicola chrysanthemi) (Dcd) in potato plant extracts. Target bacteria extracts were enriched 48 h a semi-selective broth containing polypectate under low oxygen conditions. Samples subsequently incubated with antibody-coated colour-coded microspheres...
Processed milk and products produced from bovine milk, commonly contain β-casein A1 (βCA1) A2 (βCA2). Since the presence of βCA1 is linked to intolerance digestion problems, A2A2 which only contains βCA2, proposed as a healthier alternative. To support this health claim, purity A2A2-milk has be guaranteed. In presented study, multiplex immunoassay, able distinguish between βCA2 βCA1, was developed real-life applicability shown on raw samples genotyped A1A1, A1A2 cows. Because its ability...
Summary To study the molecular regulation of nectary development, we cloned NEC1 , a gene predominantly expressed in nectaries Petunia hybrida by using differential display RT–PCR technique. The secondary structure putative protein is reminiscent transmembrane protein, indicating that incorporated into cell membrane or cytoplast membrane. Immunolocalization revealed present nectaries. Northern blot analyses showed highly tissue and weakly stamen. GUS expression driven promoter activity outer...
Flow cytometric analysis of immuno-stained cells (immuno-FCM) was compared to immunofluorescence microscopy (IF) and dilution plating on a semi-selective medium, for quantitative detection Xanthomonas axonopodis pv. phaseoli (Xap) in bean seed extracts. Cell concentrations Xap between 10³-10(7) CFU/mL were added healthy A flow cytometry sorting procedure developed separate from crude extracts confirming by PCR. FCM evaluated direct viable counting (DVC) using combinations propidium iodide...