A5057856419- Venomous Animal Envenomation and Studies
- Biochemical and Structural Characterization
- Rabies epidemiology and control
- Toxin Mechanisms and Immunotoxins
- Healthcare and Venom Research
- Monoclonal and Polyclonal Antibodies Research
- Insect and Pesticide Research
- Marine Invertebrate Physiology and Ecology
- Nicotinic Acetylcholine Receptors Study
- Protein purification and stability
- Ion channel regulation and function
- Bee Products Chemical Analysis
- Lipid Membrane Structure and Behavior
- Transgenic Plants and Applications
- Leech Biology and Applications
- Glycosylation and Glycoproteins Research
- Cellular transport and secretion
- Clostridium difficile and Clostridium perfringens research
- Bacteriophages and microbial interactions
- Antimicrobial Peptides and Activities
- Antibiotic Resistance in Bacteria
Technical University of Denmark
2019-2025
Eskişehir Osmangazi University
2019-2020
Universidad de Costa Rica
2020
Novo Nordisk Foundation
2020
Universidade de São Paulo
2020
Universidade Federal de Roraima
2020
Snakebite envenoming remains a devastating and neglected tropical disease, claiming over 100,000 lives annually causing severe complications long-lasting disabilities for many more1,2. Three-finger toxins (3FTx) are highly toxic components of elapid snake venoms that can cause diverse pathologies, including tissue damage3 inhibition nicotinic acetylcholine receptors, resulting in life-threatening neurotoxicity4. At present, the only available treatments snakebites consist polyclonal...
Abstract Oligoclonal mixtures of broadly-neutralizing antibodies can neutralize complex compositions similar and dissimilar antigens, making them versatile tools for the treatment e.g., infectious diseases animal envenomations. However, these biotherapeutics are complicated to develop due their nature. In this work, we describe application various strategies discovery cross-neutralizing nanobodies against key toxins in coral snake venoms using phage display technology. We prepare two...
Abstract Broadly-neutralizing monoclonal antibodies are of high therapeutic utility against infectious diseases caused by bacteria and viruses, as well different types intoxications. Snakebite envenoming is one such debilitating pathology, which currently treated with polyclonal derived from immunized animals. For the development novel therapies based on improved benefits, new discovery approaches for broadly-neutralizing needed. Here, we present a methodology phage display technology...
Toxin synergism is a complex biochemical phenomenon, where different animal venom proteins interact either directly or indirectly to potentiate toxicity level that above the sum of toxicities individual toxins. This provides animals possessing venoms with synergistically enhanced metabolic advantage, since less needed inflict potent toxic effects in prey and predators. Among toxins are known for interacting cytotoxins from snake venoms, phospholipases A2 bee melittin venom. These may derive...
Abstract Venomous snakes are important parts of the ecosystem, and their behavior evolution have been shaped by surrounding environments over eons. This is reflected in venoms, which typically highly adapted for biological niche, including diet defense mechanisms deterring predators. Sub-Saharan Africa rich venomous snake species, many dangerous to humans due high toxicity venoms ability effectively deliver large amounts venom into victims via bite. In this study, 26 sub-Saharan Africa's...
A major challenge in industrial pig production is the prevalence of post-weaning diarrhea (PWD) piglets, often caused by enterotoxigenic Escherichia coli (ETEC). The increased use antibiotics and zinc oxide to treat PWD has raised global concerns regarding antimicrobial resistance development environmental pollution. Still, alternative treatments targeting ETEC counteracting are largely lacking. Here, we report design a pH, temperature, protease-stable bivalent VHH-based protein BL1.2 that...
<title>Abstract</title> Snakebite envenoming remains a devastating and neglected tropical disease, claiming over 100,000 lives annually causing severe complications long-lasting disabilities for many more<sup>1,2</sup>. Three-finger toxins (3FTx) are highly toxic components of elapid snake venoms that can cause diverse pathologies, including tissue damage<sup>3</sup> inhibition nicotinic acetylcholine receptors (nAChRs) resulting in life-threatening neurotoxicity<sup>4</sup>. Currently, the...
Recycling IgG antibodies bind to their target antigen at physiological pH in the blood stream and release them upon endocytosis when levels drop, allowing be recycled into circulation via FcRn-mediated cellular pathways, while antigens undergo lysosomal degradation. This enables recycling achieve comparable therapeutic effect lower doses than non-recycling counterparts. The development of such is typically achieved by histidine doping variable regions or performing vitro antibody selection...
ABSTRACT Oligoclonal antibodies, which are carefully defined mixtures of monoclonal valuable for the treatment complex diseases, such as infectionss and cancer. In addition to these areas medicine, they could be utilized snakebite envenoming, where recombinantly produced human antibodies overcome many drawbacks accompanying traditional antivenoms. However, producing multiple individual batches in an industrial setting is associated with significant costs. Therefore, it attractive produce...
Snakebite envenoming was reintroduced as a Category A Neglected Tropical Disease by the World Health Organization in 2017. Since then, increased attention has been directed towards this affliction and development of deeper understanding how snake venoms exert their toxic effects antivenoms can counter them. However, most our vivo generated knowledge stems from use animal models which do not always accurately reflect pathogenic manifest humans. Moreover, experiments are associated with pain,...
Snakebite envenoming can be a life-threatening condition, for which emergency care is essential. The Bothrops (lancehead) genus responsible most snakebite-related deaths and permanent loss of function in human victims Latin America. spp. venom complex mixture different proteins that are known to cause local necrosis, coagulopathy, acute kidney injury. However, the long-term effects these viper envenomings have remained largely understudied.Here, we present case report 46-years old female...
Highlights•IgGs against the snake venom toxins α-cobratoxin and myotoxin II were discovered•Light-chain shuffling was used to generate IgGs with pH-dependent binding properties•Binding between IgG affects FcRn-mediated cellular handling•Cellular handing of IgG-bound is complex affected by multiple factorsSummaryImmunoglobulin G (IgG) antibodies that bind their cognate antigen in a manner (acid-switched antibodies) can release bound for degradation acidic environment endosomes, while are...
Current snakebite antivenoms are based on polyclonal animal-derived antibodies, which can neutralize snake venom toxins in envenomed victims, but also associated with adverse reactions. Therefore, several efforts within antivenom research aim to explore the utility of recombinant monoclonal such as human immunoglobulin G (IgG) routinely used clinic for other indications. In this study, feasibility using tobacco plants bioreactors expressing full-length IgG antibodies against was...
Abstract Recycling antibodies can bind to their target antigen at neutral pH in the blood stream and release them upon endocytosis when levels drop, allowing be recycled into circulation via FcRn-mediated pathway, while antigens undergo lysosomal degradation. This enables recycling achieve same therapeutic effect lower doses than non-recyclable counterparts. The development of such is typically achieved by histidine doping variable regions specific or performing vitro antibody selection...