A5072211471- Advanced Proteomics Techniques and Applications
- Inflammatory Bowel Disease
- Molecular Biology Techniques and Applications
- Mass Spectrometry Techniques and Applications
- Rheumatoid Arthritis Research and Therapies
- Pancreatic and Hepatic Oncology Research
- Pancreatitis Pathology and Treatment
- Advanced Biosensing Techniques and Applications
- Inflammasome and immune disorders
- Plant Micronutrient Interactions and Effects
- Glycosylation and Glycoproteins Research
- Genomics and Phylogenetic Studies
- Cervical Cancer and HPV Research
- Ferroptosis and cancer prognosis
- Cancer Genomics and Diagnostics
- Genetic factors in colorectal cancer
- Advanced Glycation End Products research
- Glutathione Transferases and Polymorphisms
- Galectins and Cancer Biology
- Microbial Natural Products and Biosynthesis
- Aluminum toxicity and tolerance in plants and animals
- Marine Sponges and Natural Products
- Immunodeficiency and Autoimmune Disorders
- Cell Adhesion Molecules Research
- Venomous Animal Envenomation and Studies
University of Liège
2016-2025
Digital Proteomics (United States)
2025
Universidade Federal do Rio Grande do Sul
2020
University of Genoa
2020
Despite the high prevalence of both cervico-vaginal human papillomavirus (HPV) infection and bacterial vaginosis (BV) worldwide, their causal relationship remains unclear. While BV has been presumed to be a risk factor for HPV acquisition related carcinogenesis long time, here, supported by large retrospective follow-up study (n = 6,085) extensive in vivo data using K14-HPV16 transgenic mouse model, we report novel blueprint which opposite association also exists. Mechanistically,...
Metabolic reprogramming toward aerobic glycolysis unavoidably induces methylglyoxal (MG) formation in cancer cells. MG mediates the glycation of proteins to form advanced end products (AGEs). We have recently demonstrated that MG-induced AGEs are a common feature breast cancer. Little is known regarding impact MG-mediated carbonyl stress on tumor progression. Breast tumors with presented high nuclear YAP, key transcriptional co-activator regulating growth and invasion. Elevated levels...
A disintegrin and metalloproteinase with thrombospondin type I motif (ADAMTS)2, 3, 14 are collectively named procollagen N-proteinases (pNPs) because of their specific ability to cleave the aminopropeptide fibrillar procollagens. Several reports also indicate that they could be involved in other biological processes, such as blood coagulation, development, male fertility, but potential substrates associated these activities remain unknown. Using recently described N-terminal amine isotopic...
Venom composition varies across snakes from all taxonomic levels and is influenced by the snakes’ age, habitat, diet, sexual dimorphism. The present study reports first in-depth investigation of venom in male female Bothrops moojeni (B. moojeni) (BmooM BmooF, respectively) through three proteomics approaches associated with functional, cytotoxic, immunoreactivity characterization. Compared BmooM venom, BmooF exhibited weaker hyaluronidase, metalloproteinase, phospholipase activity;...
// Arnaud Blomme 1 , Karim Fahmy Olivier Peulen Brunella Costanza Marie Fontaine 2 Ingrid Struman Dominique Baiwir 3, 4 Edwin de Pauw 3 Marc Thiry 5 Akeila Bellahcène Vincent Castronovo 1, * Andrei Turtoi Metastasis Research Laboratory, GIGA Cancer, University of Liège, 4000, Belgium Molecular Angiogenesis Research, Laboratory Mass Spectrometry, Proteomics Facility, Cell Biology, Faculty Sciences, These authors have contributed equally to this work Correspondence to: Turtoi, email:...
CRISPR-based DNA editing technologies enable rapid and accessible genome engineering of eukaryotic cells. However, the delivery genetically encoded CRISPR components remains challenging sustained Cas9 expression correlates with higher off-target activities, which can be reduced via Cas9-protein delivery. Here we demonstrate that baculovirus, alongside its cargo, used to package deliver proteins human Using protein-loaded baculovirus (pBV), or base editors proteins, leading efficient in By...
Despite recent advances in colorectal cancer (CRC) diagnosis and population screening programs, the identification of patients with preneoplastic lesions or early CRC stages remains challenging is important for reducing incidence increasing patient’s survival. We analysed 76 tissue samples originated from stages, normal inflamed mucosa by label-free proteomics. The characterisation three selected biomarker candidates was performed immunohistochemistry on an independent set precancerous...
Abstract Matrix‐assisted laser desorption/ionization (MALDI) imaging is an ideal tool to study intratumor heterogeneity (ITH) and its implication in prognostic stratification of patients. However, there are some drawbacks concerning protein identification. On the other hand, microdissection (LMD)‐based microproteomics allows retrieving thousands identifications from small tissue pieces. As a proof concept, authors combine these two complementary approaches analyze heterogeneous regions...
Over the past 40 years, proteomics, generically defined as field dedicated to identification and analysis of proteins, has tremendously gained in popularity potency through advancements genome sequencing, separative techniques, mass spectrometry, bioinformatics algorithms. As a consequence, its scope application gradually enlarged diversified meet specialized topical biomedical subjects. Although tryptic bottom-up approach is widely regarded gold standard for rapid screening complex samples,...
Mass spectrometry imaging (MSI) is an analytical technique for the unlabeled and multiplex of molecules in biological tissue sections. It therefore enables spatial molecular annotations tissues complementary to histology. has already been shown that MSI can guide subsequent material isolation technologies such as laser microdissection (LMD) enable a more in-depth characterization MSI-highlighted regions. However, with now reaching resolutions at single-cell scale, there need precise...
Abstract It is now well recognized that osteoarthritis (OA) synovial membrane presents inflammatory components. The aim of this work to provide evidence similar mechanisms exist in (n = 24) obtained from three pathologies presenting altogether an gradient: OA, chronic pyrophosphate arthropathy (CPPA) and rheumatoid arthritis (RA). Synovial biopsies were first characterized by a histological score based on hyperplasia infiltration lymphocytes, plasma cells, polymorphonuclear macrophages. All...
Regulation of mRNA translation elongation impacts nascent protein synthesis and integrity plays a critical role in disease establishment. Here, we investigate features linking regulation codon-dependent to expression homeostasis. Using knockdown models enzymes that catalyze the mcm5s2 wobble uridine tRNA modification (U34-enzymes), show gene codon content is necessary but not sufficient predict fate. While defects upon perturbation U34-enzymes are strictly dependent on content, consequences...
Metastatic hormone receptor-positive (HR+), human epidermal growth factor receptor 2-negative (HER2−) breast cancer often develops resistance to first-line treatment, typically combining cyclin-dependent kinase 4 and 6 inhibitors (CDK4/6i) with therapy (HT) [1, 2]. After an initial response, most patients become resistant, compensatory mechanisms are not fully uncovered [3]. To address this, we analyzed HR+ resistant CAMA1 747D cells using whole-exome RNA sequencing, supplemented by...
Abstract Background Perianal fistulae affects 30-34% of patients with Crohn’s Disease (CD)[1]. Scarce evidences are available on the perianal fistula pathophysiology. Bone marrow-derived Mesenchymal Stem Cells (bmMSCs) local injections have been found safe and efficient for 50% CD included in a previous prospective monocentric study[2]. The present study aims at identifying potential proteins associated closure following bmMSCs injection, using biological material collected during tract...
Proteomics, essential for understanding gene and cell functions, faces challenges with peptide loss due to adsorption onto vial surfaces, especially in samples low quantities. Using HeLa tryptic digested standard solutions, we demonstrate preferential of peptides, particularly hydrophobic ones, polypropylene (PP) vials, leading nonuniform signal loss. This phenomenon can alter protein quantification (e.g., Label-Free Quantification, LFQ) if no appropriate data processing is applied. Our...
Abstract Aetiologically linked to HPV infection, malignancies of the anal canal have substantially increased in incidence over last 20 years. Although most squamous cell carcinomas (SCCs) respond well chemoradiotherapy, about 30% patients experience a poor outcome, for undetermined reasons. Despite cumulative efforts discovering independent predictors overall survival, both nodal status and tumour size are still only reliable factors predicting patient outcome. Recent revealed that biology...
Intestinal fibrosis is a common complication of Crohn's disease [CD]. It characterised by an accumulation fibroblasts differentiating into myofibroblasts secreting excessive extracellular matrix. The potential role the intestinal epithelium in this fibrotic process remains poorly defined.We performed pilot proteomic study comparing proteome surface epithelium, isolated laser-capture microdissection, normal and zones resected ileal CD strictures [13 collected five patients]. Proteins...
Streptomyces lunaelactis strains have been isolated from moonmilk deposits, which are calcium carbonate speleothems used for centuries in traditional medicine their antimicrobial properties. Genome mining revealed that these a remarkable example of species with huge heterogeneity regarding content biosynthetic gene clusters (BGCs) specialized metabolite production. BGC 28a is one the cryptic BGCs only carried by subgroup S. silico analysis predicted production nonribosomal peptide...
Abstract Background Increased permeability, mucosal barrier and epithelial anomalies (crypt distortion, wider crypt lumen, gaps) as well altered tight junctions have been described in active ulcerative colitis (UC) Crohn’s Disease (CD). Probe based Confocal laser endomicroscopy (pCLE) is able to monitor vivo1. This study aimed at comparing the proteomic profiles of colonic epithelium associated with pCLE UC, CD non-IBD subjects. Methods Biopsies were collected IBDs endoscopic remission...
Based on genetics and natural history, Crohn's disease can be separated into two entities, an ileal a colonic disease. Protein-based approaches are needed to elucidate whether such subphenotypes related distinct pathophysiological processes.The proteome of ulcer edges was compared with that paired control tissue samples [n = 32 biopsies] by differential proteomics in the ileum colon patients 16]. The results were analysed using hypothesis-driven approach [based literature] hypothesis-free...
A subset of Crohn's disease (CD) patients experiences mid/long-term remission after infliximab withdrawal. Biomarkers are needed to identify those patients.
An inflamed synovial membrane plays a major role in joint destruction and is characterized by immune cells infiltration fibroblast proliferation. This proteomic study considers the inflammatory process at molecular level analyzing biopsies presenting histological continuum throughout different arthritis diseases. Knee were obtained from osteoarthritis (OA; n = 9), chronic pyrophosphate arthropathy (CPPA; 7) or rheumatoid (RA; 8) patients. The score was determined using semi-quantitative...