A5075707600- Osteoarthritis Treatment and Mechanisms
- Inflammatory mediators and NSAID effects
- Cell Adhesion Molecules Research
- Proteoglycans and glycosaminoglycans research
- Rheumatoid Arthritis Research and Therapies
- Autophagy in Disease and Therapy
- Endoplasmic Reticulum Stress and Disease
- Musculoskeletal synovial abnormalities and treatments
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Orthopedic Infections and Treatments
- Bone Metabolism and Diseases
- Tendon Structure and Treatment
- Toxoplasma gondii Research Studies
- Bone and Joint Diseases
- Protease and Inhibitor Mechanisms
- Cardiac Valve Diseases and Treatments
- Connective tissue disorders research
- Hip disorders and treatments
- Pulmonary Hypertension Research and Treatments
- Total Knee Arthroplasty Outcomes
- Beetle Biology and Toxicology Studies
- Heparin-Induced Thrombocytopenia and Thrombosis
- Peroxisome Proliferator-Activated Receptors
- Bacterial Identification and Susceptibility Testing
- Sphingolipid Metabolism and Signaling
Centre Hospitalier Universitaire de Liège
2015-2024
University of Liège
2015-2024
Abstract CEMIP (for “Cell migration-inducing protein” also called KIAA1199 and Hybid for “Hyaluronan-binding protein”) expression is increased in cancers described as a regulator of cell survival, growth invasion. In rheumatoid arthritis, referred to an angiogenic marker participates hyaluronic acid degradation. this study, investigated healthy osteoarthritis (OA) cartilage from human mouse. Its role OA physiopathology deciphered, specifically chondrocytes proliferation dedifferentiation the...
Osteoarthritis (OA) synovial membrane is mainly characterized by low-grade inflammation, hyperplasia with increased cell proliferation and fibrosis. We previously underscored a critical role for CEMIP in fibrosis of OA cartilage. However, its remains unknown. An vitro model fibroblast-like synoviocytes from patients an vivo collagenase-induced mice were used to evaluate CEMIP-silencing effects on Our results showed that i. expression was human mouse inflamed membrane; ii. regulated the...
Abstract Osteoarthritis is characterized by structural alteration of joints. Fibrosis the synovial tissue often detected and considered one main causes joint stiffness pain. In our earlier proteomic study, increased levels vitronectin (VTN) fragment (amino acids 381–397) were observed in serum osteoarthritis patients. this work, affinity for integrins its putative role TGF-β1 activation investigated. A competition study determined interaction VTN (381–397 a.a.) with α V β 6 integrin....
To date, precise roles of EMD (emerin) remain poorly described. In this paper, we investigated the role in C16-ceramide autophagy pathway. Ceramides are bioactive signaling molecules acting notably regulation cell growth, differentiation, or death. However, mechanisms by which they mediate these pathways not fully understood. We found that induces phosphorylation on its LEM domain through PRKACA. Upon ceramide treatment, phosphorylated binds MAP1LC3B leading to an increase autophagosome...
Mitral regurgitation is a frequent valvular heart disease affecting around 2.5 % of the population with prevalence directly related to aging. Degeneration mitral valve broadly considered as passive ongoing pathophysiological process and little known about its physiological deregulation. The purpose this study was highlight new biomarkers in order decipher underlying pathological mechanism well allow diagnosis monitoring disease. Modulation various blood proteins expression examined patients...
An inflamed synovial membrane plays a major role in joint destruction and is characterized by immune cells infiltration fibroblast proliferation. This proteomic study considers the inflammatory process at molecular level analyzing biopsies presenting histological continuum throughout different arthritis diseases. Knee were obtained from osteoarthritis (OA; n = 9), chronic pyrophosphate arthropathy (CPPA; 7) or rheumatoid (RA; 8) patients. The score was determined using semi-quantitative...
Glucocorticoid-induced leucine zipper (GILZ) is a mediator of the anti-inflammatory activities glucocorticoids. However, GILZ deletion does not impair exogenous glucocorticoids in mice arthritis models and could also mediate some glucocorticoid-related adverse events. Osteoarthritis (OA) metabolic disorder that partly attributed to adipokines such as leptin, we previously observed induced leptin secretion OA synovial fibroblasts. The purpose this study was position through its involvement...
We previously reported 18FPRGD2 uptake by the coxofemoral lining, intervertebral discs and facet joint osteophytes in OA using PET/SCAN imaging. However, molecular mechanism which PRGD2 tracer interacts with tissues remains unclear. As ligands are expected to belong RGD-specific integrin family, purpose of this study was (i) determine complexes display highest affinity for PRGD2-based ligands, (ii) analyze expression relevant tissues, (iii) test regulation chondrocytes OA-related stimuli...
Inhibition of proapoptotic pathways in synovial fibroblasts is one the major causes proliferation and hyperplasia rheumatic diseases. We have shown previously that NF-κB inhibitor BAY 11-7085, through inactivation PPAR-γ, induces apoptosis human fibroblasts. In this work we showed 11-7085 induced autophagy preceded 11-7085-induced apoptosis. Of interest, Serine 211 phosphorylation degradation glucocorticoid receptor (GR). Glucocorticoid prednisolone both activation GR, as well cell death was...
// Biserka Relic 1 , Edith Charlier Celine Deroyer Olivier Malaise Yannick Crine Sophie Neuville Philippe Gillet 2 Dominique de Seny and Michel G. Department of Rheumatology, GIGA Research, University Hospital Sart-Tilman, Liege, Belgium Orthopedic Surgery, Correspondence to: Relic, email: Biserka.Relic@chuliege.be Keywords: p62/SQSTM1 phosphorylation; proteasome; autophagy; serum starvation; RAD23B Received: May 25, 2018 Accepted: October 24, Published: November 09, ABSTRACT Phosphorylation...
Osteoarthritis (OA) is recognized as being a cellular senescence-linked disease. Intra-articular injections of glucocorticoids (GC) are frequently used in knee OA to treat synovial effusion but face controversies about toxicity. We investigated the influence GC on senescence hallmarks and induction fibroblast-like synoviocytes (FLS) from patients mesenchymal stem cells (MSC). Cellular was assessed via proliferation rate, β-galactosidase staining, DNA damage CKI expression (p21, p16INK4A)....
Autophagy receptor p62/SQSTM1 signals a complex network that links autophagy-lysosomal system to proteasome. Phosphorylation of p62 on Serine 349 (P-Ser349 p62) is involved in cell protective, antioxidant pathway. We have shown previously P-Ser349 occurs and rapidly degraded during human synovial fibroblasts autophagy. In this work we observed fingolimod (FTY720), used as medication for multiple sclerosis, induced coordinated expression p62, inhibitory TFEB form, phosphorylated 211 (P-Ser211...
Angina is chest pain induced by ischemia of the heart muscle, generally due to obstruction or spasm coronary arteries. People that suffer from average severe cases angina have an increased percentage death before age 55, usually around 60%. Therefore, prevention major complications, optimizing diagnosis, prognosis and therapeutics are primary importance. The main objective this study was uncover biomarkers comparing serum protein profiles patients suffering stable unstable controls. We...
<h3>Background:</h3> A strong crosstalk exists between endoplasmic reticulum (ER) stress and inflammation in synovium of arthritis patients. In addition to their primary role protein folding, various ER chaperone proteins can contribute cell survival, amplifying immunogenicity when is triggered pathological situations. Moreover, abrogates contacts macrophage-like synoviocytes, therefore disturbing the lining layer structure[1]. Simultaneously, fibroblast-like synoviocytes (FLS) take...
<h3>Background:</h3> Scleroderma or systemic sclerosis (SSc) is a rare multisystemic autoimmune disease. It initiates with endothelial damage and inflammatory infiltrate, leading to fibrosis in various organs. Activation of different immune cells induces the transformation fibroblasts into myofibroblasts, responsible for excessive production extracellular matrix components reactive oxygen species. A better understanding pathophysiology tissue necessary identify therapeutic targets. CEMIP...
<title>Abstract</title> A strong crosstalk exists between endoplasmic reticulum (ER) stress and synovitis. Several ER chaperone proteins, besides their function in protein folding, can enhance inflammation immunogenicity when secreted. This research aims at localizing quantifying by immunohistochemistry (IHC) proteins (BiP, HYOU1, MANF, PDIA4, GANAB, HSP90B1, TXNDC5, DNAJB11, LMAN1, ERP29 CALR) synovial membranes provided from patients with osteoarthritis (OA; n = 9), chronic pyrophosphate...