A5091705546- Prostate Cancer Treatment and Research
- Cancer, Hypoxia, and Metabolism
- Cancer, Lipids, and Metabolism
- PARP inhibition in cancer therapy
- Receptor Mechanisms and Signaling
- Histone Deacetylase Inhibitors Research
- Mass Spectrometry Techniques and Applications
- Advanced Proteomics Techniques and Applications
- Metabolism, Diabetes, and Cancer
- TGF-β signaling in diseases
- Cancer Research and Treatments
- Cancer Genomics and Diagnostics
- Cell Adhesion Molecules Research
- Protein Degradation and Inhibitors
- Peptidase Inhibition and Analysis
- Cancer-related gene regulation
- Mitochondrial Function and Pathology
- Ubiquitin and proteasome pathways
- Cancer Cells and Metastasis
- Advanced Glycation End Products research
- Wnt/β-catenin signaling in development and cancer
- Lipid metabolism and biosynthesis
- Colorectal Cancer Treatments and Studies
- Fibroblast Growth Factor Research
- Bone and Dental Protein Studies
University of Liège
2014-2025
Walloon Excellence in Lifesciences and Biotechnology
2025
Cancer Research UK Scotland Institute
2018-2023
University of Glasgow
2021-2022
Abstract Despite the clinical success of Androgen Receptor (AR)-targeted therapies, reactivation AR signalling remains main driver castration-resistant prostate cancer (CRPC) progression. In this study, we perform a comprehensive unbiased characterisation LNCaP cells chronically exposed to multiple inhibitors (ARI). Combined proteomics and metabolomics analyses implicate an acquired metabolic phenotype common in ARI-resistant associated with perturbed glucose lipid metabolism. To exploit...
Metabolic reprogramming toward aerobic glycolysis unavoidably induces methylglyoxal (MG) formation in cancer cells. MG mediates the glycation of proteins to form advanced end products (AGEs). We have recently demonstrated that MG-induced AGEs are a common feature breast cancer. Little is known regarding impact MG-mediated carbonyl stress on tumor progression. Breast tumors with presented high nuclear YAP, key transcriptional co-activator regulating growth and invasion. Elevated levels...
Background Breast cancer is a leading malignancy affecting the female population worldwide. Most morbidity caused by metastases that remain incurable to date. TGF-β1 has been identified as key driving force behind metastatic breast cancer, with promising therapeutic implications. Methods and Findings Employing immunohistochemistry (IHC) analysis, we report, our knowledge for first time, asporin overexpressed in stroma of most human cancers not expressed normal tissue. In vitro, secreted...
Abstract IRE1α is constitutively active in several cancers and can contribute to cancer progression. Activated cleaves XBP1 mRNA, a key step production of the transcription factor XBP1s. In addition, select mRNAs through regulated IRE1α-dependent decay (RIDD). Accumulating evidence implicates regulation lipid metabolism. However, roles XBP1s RIDD this process remain ill-defined. study, transcriptome lipidome profiling triple negative breast cells subjected pharmacological inhibition reveals...
Pancreatic ductal adenocarcinoma (PDAC) remains a deadly malignancy with no efficient therapy available up‐to‐date. Glycolysis is the main provider of energetic substrates to sustain cancer dissemination PDAC. Accordingly, altering glycolytic pathway foreseen as sound approach trigger pancreatic regression. Here, we show for first time that high transforming growth factor beta‐induced (TGFBI) expression in PDAC patients associated poor outcome. We demonstrate that, although usually secreted...
// Arnaud Blomme 1 , Karim Fahmy Olivier Peulen Brunella Costanza Marie Fontaine 2 Ingrid Struman Dominique Baiwir 3, 4 Edwin de Pauw 3 Marc Thiry 5 Akeila Bellahcène Vincent Castronovo 1, * Andrei Turtoi Metastasis Research Laboratory, GIGA Cancer, University of Liège, 4000, Belgium Molecular Angiogenesis Research, Laboratory Mass Spectrometry, Proteomics Facility, Cell Biology, Faculty Sciences, These authors have contributed equally to this work Correspondence to: Turtoi, email:...
Colorectal cancer (CRC) cells are traditionally considered unresponsive to TGFβ due mutations in the receptors and/or downstream signaling molecules. influences CRC only indirectly via stromal cells, such as cancer-associated fibroblasts. However, cell ability directly respond currently remains unexplored. This represents a missed opportunity for diagnostic and therapeutic interventions. Methods: We examined whether from primary liver metastases by inducing TGFβ-induced protein ig-h3 (TGFBI)...
Dysregulation of mRNA translation, including preferential translation with complex 5'-UTRs such as the MYC oncogene, is recognized an important mechanism in cancer. In this study, we show that both human and murine chronic lymphocytic leukemia (CLL) cells display a high rate, which can be inhibited by synthetic flavagline FL3, prohibitin (PHB)-binding drug. A multiomics analysis consisting pulsed SILAC, RNA sequencing polysome profiling performed CLL patient samples cell lines treated FL3...
Transfer RNA dynamics contribute to cancer development through regulation of codon-specific messenger translation. Specific aminoacyl-tRNA synthetases can either promote or suppress tumourigenesis. Here we show that valine synthetase (VARS) is a key player in the codon-biased translation reprogramming induced by resistance targeted (MAPK) therapy melanoma. The proteome rewiring patient-derived MAPK therapy-resistant melanoma biased towards usage and coincides with upregulation cognate tRNAs...
Myoferlin is a member of the ferlin family proteins that participate in plasma membrane fusion, repair, and endocytosis. While some reports have implicated myoferlin cancer, extent its expression contributions to cancer are not well established. In this study, we show overexpressed human breast cancers it has critical role controlling degradation epidermal growth factor (EGF) receptor (EGFR) after activation internalization cells. depletion blocked EGF-induced cell migration...
For many years the laboratory mouse has been used as standard model for in vivo oncology research, particularly development of novel PET tracers, but growth tumors on chicken chorioallantoic membrane (CAM) provides a more rapid, low cost, and ethically sustainable alternative. first time, to our knowledge, we demonstrate feasibility CT imaging U87 glioblastoma tumor CAM, with aim applying this screening tracers.U87 cells were implanted CAM at day 11 after fertilization imaged 18. A...
Tumor heterogeneity is a major obstacle for developing effective anticancer treatments. Recent studies have pointed to large stochastic genetic within cancer lesions, where no pattern seems exist that would enable more structured targeted therapy approach. Because date similar information available at the protein (phenotype) level, we employed matrix assisted laser desorption ionization (MALDI) image-guided proteomics and explored of extracellular membrane subproteome in unique collection...
Abstract Inhibition of the androgen receptor (AR) is main strategy to treat advanced prostate cancers. AR-independent treatment-resistant cancer a major unresolved clinical problem. Patients with alterations in canonical WNT pathway genes, which lead β-catenin activation, are refractory AR-targeted therapies. Here, using clinically relevant murine models, we investigated significance activation progression and treatment resistance. β-Catenin independent cell origin, cooperated Pten loss...
Article11 January 2022Open Access Source DataTransparent process THEM6-mediated reprogramming of lipid metabolism supports treatment resistance in prostate cancer Arnaud Blomme Corresponding Author [email protected] orcid.org/0000-0003-4183-8726 CRUK Beatson Institute, Garscube Estate, Glasgow, UK Contribution: Conceptualization, Data curation, Formal analysis, Investigation, Methodology, Writing - original draft, Project administration, review & editing Search for more papers by this...
Functional targeted therapy has unfortunately failed to improve the outcome of glioblastoma patients. Success stories evidenced by use antibody-drug conjugates in other tumor types are encouraging, but targets specific and accessible through bloodstream remain scarce. In current work, we have identified characterized novel proteins using an innovative proteomic approach on six human glioblastomas; corresponding data been deposited PRIDE database identifier PXD001398. Among several clusters...
Highlights•Glycolytic mutant KRAS display higher MGO stress than wild-type CRC cells•MGO is a potent inducer of AKT signaling in induces resistance to anti-EGFR therapy setting•Carnosine, an scavenger, sensitizes tumors therapySummaryThe use cetuximab anti-epidermal growth factor receptor (anti-EGFR) antibodies has opened the era targeted and personalized colorectal cancer (CRC). Poor response rates have been unequivocally shown are even observed majority tumors. Therefore, patient selection...
Abstract Androgen deprivation therapy (ADT) is the standard of care for treatment nonresectable prostate cancer. Despite high efficiency, most patients ultimately develop lethal castration-resistant cancer (CRPC). In this study, we performed a comparative proteomic analysis three in vivo, androgen receptor (AR)-responsive orthograft models matched hormone-naïve and CRPC. Differential revealed that distinct molecular mechanisms, including amino acid (AA) fatty metabolism, are involved...
The identification of specific biomarkers obtained directly from human pathological lesions remains a major challenge, because the amount tissue available is often very limited. We have developed novel, comprehensive, and efficient method permitting absolute quantification potentially accessible proteins in such precious samples. This protein subclass comprises cell membrane associated extracellular proteins, which are reachable by systemically deliverable substances hence especially...
Research Article14 March 2018Open Access Source DataTransparent process Sprouty2 loss-induced IL6 drives castration-resistant prostate cancer through scavenger receptor B1 Rachana Patel Corresponding Author [email protected] orcid.org/0000-0003-2147-4080 Cancer UK Beatson Institute, Glasgow, Search for more papers by this author Janis Fleming Ernest Mui Institute of Sciences, Carolyn Loveridge Peter Repiscak Arnaud Blomme Victoria Harle Mark Salji Imran Ahmad Katy Teo Freddie C Hamdy...