A5018552299- Gold and Silver Nanoparticles Synthesis and Applications
- Spectroscopy Techniques in Biomedical and Chemical Research
- Advanced biosensing and bioanalysis techniques
- Biosensors and Analytical Detection
- Spectroscopy and Chemometric Analyses
- Nanocluster Synthesis and Applications
- Advanced Biosensing Techniques and Applications
- Protein Interaction Studies and Fluorescence Analysis
- Crystallization and Solubility Studies
- X-ray Diffraction in Crystallography
- Advanced Nanomaterials in Catalysis
- RNA Interference and Gene Delivery
- Metabolomics and Mass Spectrometry Studies
- Identification and Quantification in Food
- Quantum Dots Synthesis And Properties
- Nanofabrication and Lithography Techniques
- Electrochemical Analysis and Applications
- Mass Spectrometry Techniques and Applications
- Nanoparticle-Based Drug Delivery
- Terahertz technology and applications
- DNA and Nucleic Acid Chemistry
- Plant and animal studies
- Cancer, Lipids, and Metabolism
- Bacterial Identification and Susceptibility Testing
- Molecular Biology Techniques and Applications
University of Strathclyde
2016-2025
Fraunhofer Centre for Applied Photonics
2021-2023
The University of Texas at El Paso
2023
Stockholm University
2023
Curtin University
2023
Michigan State University
2023
Rutherford Appleton Laboratory
2023
Confederazione Nazionale dell'Artigianato e Della Piccola e Media Impresa
2023
Columbia University
2023
Xiamen University
2023
The discovery of the enhancement Raman scattering by molecules adsorbed on nanostructured metal surfaces is a landmark in history spectroscopic and analytical techniques. Significant experimental theoretical effort has been directed toward understanding surface-enhanced (SERS) effect demonstrating its potential various types ultrasensitive sensing applications wide variety fields. In 45 years since discovery, SERS blossomed into rich area research technology, but additional efforts are still...
Successful pathogen detection is crucial for public health as the threat of infectious disease dramatically increasing globally due to bacteria developing resistance many antimicrobial drugs. The increase in bacterial infections has led urgent demands simpler, faster, and more reliable methods be developed allowing most appropriate therapy provided. Surface enhanced Raman scattering (SERS) an analytical technique which gained a great deal interest biosensing its sensitivity, selectivity,...
Abstract Despite the clinical success of Androgen Receptor (AR)-targeted therapies, reactivation AR signalling remains main driver castration-resistant prostate cancer (CRPC) progression. In this study, we perform a comprehensive unbiased characterisation LNCaP cells chronically exposed to multiple inhibitors (ARI). Combined proteomics and metabolomics analyses implicate an acquired metabolic phenotype common in ARI-resistant associated with perturbed glucose lipid metabolism. To exploit...
Abstract Inspired by the role of intracellular liquid-liquid phase separation (LLPS) in formation membraneless organelles, there is great interest developing dynamic compartments formed LLPS intrinsically disordered proteins (IDPs) or short peptides. However, molecular mechanisms underlying biomolecular condensates have not been fully elucidated, rendering on-demand design synthetic with tailored physico-chemical functionalities a significant challenge. To address this need, here we library...
Oligonucleotide−gold nanoparticle (OGN) conjugates are powerful tools for the detection of target DNA sequences due to unique properties conferred upon oligonucleotide by nanoparticle. Practically all research and applications these have used gold nanoparticles exclusion other noble metal nanoparticles. Here we report synthesis oligonucleotide−silver (OSN) demonstrate their use in a sandwich assay format. The OSN practically identical analogues vastly greater extinction coefficient both...
The labeling of biological species using dyes has become common practice to aid in their detection, and immediate positive identification specific high dilution is a key requirement. Here the detection by surface-enhanced resonance Raman scattering (SERRS) eight commercially available dye labels (ROX, rhodamine 6G, HEX, FAM, TET, Cy3, Cy5, TAMRA) attached oligonucleotide strands reported. Each was easily detected SERRS from silver nanoparticles produce unique, molecularly spectrum....
SESORS - Surface enhanced spatially offset Raman spectroscopy–imaging is explored for the first time in this study. Multiplexed surface scattering (SERS) signals have been recovered non-invasively from a depth of 20 mm tissues and reconstructed to produce false colour image. Four unique 'flavours' SERS nanoparticles (NPs) were injected into × 50 porcine tissue block at corners 10 square. A transmission data cube was acquired over an 11 pixel grid made up 2 steps. The using peak intensities...
We report the use of a SERS based DNA detection assay for multiplexed, quantification three bacterial meningitis pathogens.
Abstract Colorimetric detection of analytes using gold nanoparticles along with surface‐enhanced Raman spectroscopy (SERS) are areas intense research activity since they both offer sensing very low concentrations target species. Multimodal promotes the simultaneous a sample by combination different techniques; consequently, surface chemistry design in development multimodal nanosensors is important for rapid and sensitive evaluation diverse analytical methods. Herein it shown that...
The multiplexed detection of biological analytes from complex mixtures is crucial importance for the future intelligent management and disease. This review focuses on recent advances in use surface enhanced Raman scattering (SERS) spectroscopy as an analytical technique that can deliver a variety target increasingly media. SERS has developed multipelxed custom dye molecules to biomolecules such DNA proteins. Recent work also shown capability multiplexing vivo well vitro applications.
The ability to develop new and sensitive methods of biomolecule detection is crucial the advancement pre-clinical disease diagnosis effective patient specific treatment. Surface enhanced Raman scattering (SERS) an optical spectroscopy amenable this goal, as it capable extremely multiplexed analysis. This perspective highlights where SERS has been successfully used detect target biomolecules, specifically DNA proteins, in vivo analysis utilised. future development discussed emphasis placed on...
Establishing a definitive diagnosis of pneumonia using conventional tests is difficult and expensive. Lateral flow immunoassays (LFIAs) are an advantageous point care (POC) test option, but they have some limitations in terms detection quantification. In this work we developed lateral immunoassay for the ultrasensitive penumolysin employing plasmonic Surface-Enhanced Resonance Raman Scattering (SERRS) tag as labelled probe. The combination Au@Ag core–shell nanoparticles platform Rhodamine B...
Surface-enhanced Raman scattering (SERS) is a powerful and sensitive technique for the detection of fingerprint signals molecules investigation series surface chemical reactions. Many studies introduced quantitative applications SERS in various fields, several methods have been implemented each specific application, ranging performance characteristics, analytes used, instruments, analytical matrices. In general, very few validated according to international guidelines. As consequence,...
Carboxylesterases (CEs) are a class of enzymes that catalyze the hydrolysis esters in variety endogenous and exogenous molecules. CEs play an important role drug metabolism, onset progression disease, can be harnessed for prodrug activation strategies. As such, regulation is clinical pharmaceutical consideration. Here, we report first ratiometric sensor CE activity using Raman spectroscopy based on bisarylbutadiyne scaffold. The was shown to highly sensitive specific detection had low...