A5102801102- Spectroscopy Techniques in Biomedical and Chemical Research
- Gold and Silver Nanoparticles Synthesis and Applications
- Advanced biosensing and bioanalysis techniques
- Biosensors and Analytical Detection
- Extracellular vesicles in disease
- Spectroscopy and Chemometric Analyses
- RNA Interference and Gene Delivery
- MicroRNA in disease regulation
- Nanoparticle-Based Drug Delivery
- Advanced Biosensing Techniques and Applications
- Digital Imaging for Blood Diseases
- Curcumin's Biomedical Applications
- Alzheimer's disease research and treatments
- AI in cancer detection
- Infrared Thermography in Medicine
- Quantum Dots Synthesis And Properties
- Peptidase Inhibition and Analysis
- Cancer Immunotherapy and Biomarkers
- Advanced X-ray and CT Imaging
- Molecular Biology Techniques and Applications
- Ferroptosis and cancer prognosis
- Amyotrophic Lateral Sclerosis Research
- HER2/EGFR in Cancer Research
- Cancer Cells and Metastasis
- Cancer, Stress, Anesthesia, and Immune Response
Istituti Clinici Scientifici Maugeri
2018-2025
Istituto di Ricovero e Cura a Carattere Scientifico San Raffaele
2024
Vita-Salute San Raffaele University
2024
Istituti di Ricovero e Cura a Carattere Scientifico
2024
Luigi Sacco Hospital
2021
Weatherford College
2020
Don Carlo Gnocchi Foundation
2011-2018
Mylan (Switzerland)
2014-2015
International Flame Research Foundation
2015
University of Milano-Bicocca
2011
The use of exosomes for diagnostic and disease monitoring purposes is becoming particularly appealing in biomedical research because the possibility to study directly biological fluids some features related organs from which originate. A paradigmatic example are brain-derived that can be found plasma used as a direct read-out status central nervous system (CNS). Inspired by recent remarkable development plasmonic biosensors, we have designed surface plasmon resonance imaging (SPRi) assay...
Abstract Extracellular vesicles (EVs) from mesenchymal stromal cells (MSC) are emerging as valuable therapeutic agents for tissue regeneration and immunomodulation, but their clinical applications have so far been limited by the technical restraints of current isolation characterisation procedures. This study shows first time successful application Raman spectroscopy label-free, sensitive reproducible means carrying out routine bulk MSC-derived before use in vitro or vivo , thus promoting...
Surface-enhanced Raman scattering (SERS) is a powerful and sensitive technique for the detection of fingerprint signals molecules investigation series surface chemical reactions. Many studies introduced quantitative applications SERS in various fields, several methods have been implemented each specific application, ranging performance characteristics, analytes used, instruments, analytical matrices. In general, very few validated according to international guidelines. As consequence,...
Neurofilament light chains (NfL) are neuron-specific cytoskeletal proteins whose plasmatic concentrations have been explored as a clinically useful marker in several types of dementia. Plasma NfL extremely low, and just two assays commercially available for their study: one based on the SiMoA technology Ella. We thus studied plasma levels with both platforms to check correlation between them assess potential diagnosis neurodegeneration. were measured 50 subjects: 18 healthy controls, 20...
Triple negative breast cancer (TNBC) is a highly aggressive, invasive, and metastatic tumor. Although it reported to be sensitive cytotoxic chemotherapeutics, frequent relapse chemoresistance often result in treatment failure. In this study, we developed biomimetic nanodrug consisting of self-assembling variant (HFn) human apoferritin loaded with curcumin. HFn nanocage improved the solubility, chemical stability, bioavailability curcumin, allowing us reliably carry out several experiments...
The variable configuration of Raman spectroscopic platforms is one the major obstacles in establishing spectroscopy as a valuable physicochemical method within real-world scenarios such clinical diagnostics. For real world applications like diagnostic classification, models should ideally be usable to predict data from different setups. Whether it done by training rugged model with many setups or primary-replica strategy where are developed on 'primary' setup and test generated 'replicate'...
Breast microcalcifications are a common mammographic finding. Microcalcifications considered suspicious signs of breast cancer and biopsy is required, however, diagnosed in only few patients. Reducing unnecessary biopsies rapid characterization unmet clinical needs. In this study, 473 detected on specimens from 56 patients were characterized entirely by Raman mapping confirmed X-ray scattering. malignant samples generally more homogeneous, crystalline, less substituted crystal lattice...
Heamatopoietic cancer cells from patients were objectively and accurately recognized by high-resolution Raman imaging their characteristic spectra.
Cancer-associated fibroblasts (CAFs) are key actors in regulating cancer progression. They promote tumor growth, metastasis formation, and induce drug resistance. For these reasons, they emerging as potential therapeutic targets. Here, with the aim of developing CAF-targeted delivery agents, we functionalized H-ferritin (HFn) nanocages fibroblast activation protein (FAP) antibody fragments. Functionalized (HFn-FAP) have significantly higher binding FAP
Molecular imaging techniques are essential tools for better investigating biological processes and detecting disease biomarkers with improvement of both diagnosis therapy monitoring. Often, a single technique is not sufficient to obtain comprehensive information at different levels. Multimodal diagnostic probes key enable across multiple scales. The direct registration in vivo markers ex the cellular level probe still challenging. Fluorinated (19F) have been increasingly showing promising...
Identification of reliable and accessible biomarkers to characterize ischemic stroke patients’ prognosis remains a clinical challenge. Neurofilament light chain (NfL) glial fibrillary acidic protein (GFAP) are markers brain injury, detectable in blood by high-sensitive technologies. Our aim was measure serum NfL GFAP after stroke, evaluate their correlation with functional outcome the scores rehabilitation scales at 3-month follow-up. Stroke patients were prospectively enrolled longitudinal...
The global demographic shift towards an aging population is generating a rise in neurodegenerative conditions, with Alzheimer's disease (AD) as the most prominent problem. In this landscape, use of natural supplements has garnered attention for their potential dementia prevention. Curcumin (Cur), derived from Curcuma longa, demonstrated promising pharmacological effects against AD by reducing levels inflammatory mediators. However, its clinical efficacy hindered poor solubility and...
Identifying physiologically relevant binding partners of amyloid-β (Aβ) that modulate in vivo fibril formation may yield new insights into Alzheimer's disease (AD) etiology. Human cathelicidin peptide, LL-37, is an innate immune effector and modulator, ubiquitous human tissues expressed myriad cell types.We present vitro experimental evidence discuss findings supporting a novel hypothesis LL-37 binds to Aβ42 can Aβ formation.Specific interactions between (with different aggregation states,...
The goal of this work is to develop an innovative approach for the coating gold nanoparticles (AuNPs) with a synthetic functional copolymer. This stable thickness few nanometers provides, at same time, stabilization and functionalization particles. polymeric consists backbone polydimethylacrylamide (DMA) functionalized alkyne monomer that allows binding azido modified molecules by Cu(I)-catalyzed azide/alkyne 1,3-dipolar cycloaddition (CuAAC, click chemistry). thin polymer layer on surface...
Indocyanine green (ICG) is a fluorescent dye with strong emission in the near-infrared spectral range that allows deep signal penetration and minimal interference of tissue autofluorescence. It has been employed clinics for different applications, among which more interesting certainly fluorescence image-guided surgery. This technique found wide application surgical oncology lymph node mapping or laparoscopic Despite ICG being useful tracking loco-regional nodes, it does not provide any...
Immune inflammatory biomarkers are easily obtained and inexpensive blood-based parameters that recently showed prognostic predictive value in many solid tumors. In this study, we aimed to investigate the role of these predicting distant relapse breast cancer patients treated with neoadjuvant chemotherapy (NACT). All who referred our Breast Unit underwent NACT were retrospectively reviewed. The pre-treatment neutrophil-to-lymphocyte ratio (NLR), platelet-to-lymphocyte (PLR),...
Extracellular vesicles (EVs) have mostly been investigated as carriers of biological therapeutics such proteins and RNA. Nevertheless, small-molecule drugs natural or synthetic origin also loaded into EVs, resulting in an improvement their therapeutic properties. A few methods employed for EV cargo loading, but poor yield drastic modifications remain unsolved challenges. We tested a different strategy based on temporary pH alteration through incubation EVs with alkaline sodium carbonate,...
Among polymeric nanoparticles designed for cancer therapy, PLGA have become one of the most popular devices chemotherapeutic-based nanoformulations against several kinds malignant diseases. Promising properties, including long-circulation time, enhanced tumor localization, interference with "multidrug" resistance effects, and environmental biodegradability, often result in an improvement drug bioavailability effectiveness. In present work, we synthesized...