A5003400128- Nanoparticle-Based Drug Delivery
- Nanoplatforms for cancer theranostics
- Iron Metabolism and Disorders
- Cancer Cells and Metastasis
- HER2/EGFR in Cancer Research
- Epigenetics and DNA Methylation
- Peptidase Inhibition and Analysis
- RNA Interference and Gene Delivery
- Chronic Lymphocytic Leukemia Research
- Photodynamic Therapy Research Studies
- Signaling Pathways in Disease
- Breast Cancer Treatment Studies
- Cancer Treatment and Pharmacology
- Advanced biosensing and bioanalysis techniques
- Chemotherapy-induced cardiotoxicity and mitigation
- Extracellular vesicles in disease
- MicroRNA in disease regulation
- Breast Lesions and Carcinomas
- Brain Metastases and Treatment
- Advanced Drug Delivery Systems
- Inflammatory Bowel Disease
- Microscopic Colitis
- Eosinophilic Esophagitis
- Body Composition Measurement Techniques
- Metabolomics and Mass Spectrometry Studies
University of Milan
2020-2024
Luigi Sacco Hospital
2019-2022
Mario Negri Institute for Pharmacological Research
2019
Cancer-associated fibroblasts (CAFs) are key actors in regulating cancer progression. They promote tumor growth, metastasis formation, and induce drug resistance. For these reasons, they emerging as potential therapeutic targets. Here, with the aim of developing CAF-targeted delivery agents, we functionalized H-ferritin (HFn) nanocages fibroblast activation protein (FAP) antibody fragments. Functionalized (HFn-FAP) have significantly higher binding FAP
Indocyanine green (ICG) is a fluorescent dye with strong emission in the near-infrared spectral range that allows deep signal penetration and minimal interference of tissue autofluorescence. It has been employed clinics for different applications, among which more interesting certainly fluorescence image-guided surgery. This technique found wide application surgical oncology lymph node mapping or laparoscopic Despite ICG being useful tracking loco-regional nodes, it does not provide any...
Introduction: Photodynamic Therapy (PDT) is a promising, minimally invasive treatment for cancer with high immunostimulatory potential, no reported drug resistance, and reduced side effects. Indocyanine Green (ICG) has been used as photosensitizer (PS) PDT, although its poor stability low tumor-target specificity strongly limit efficacy. To overcome these limitations, ICG can be formulated tumor-targeting nanoparticle (NP). Methods: We nanoformulated into recombinant heavy-ferritin nanocages...
One of the goals pharmaceutical sciences is amelioration targeted drug delivery. In this context, nanocarrier-dependent transportation represents an ideal method for confronting a broad range human disorders. study, we investigated possibility improving selective release anti-cancer paclitaxel (PTX) in gastro-intestinal tract by encapsulating it into biodegradable nanoparticles made FDA-approved poly(lactic-co-glycolic acid) (PLGA) and coated with polyethylene glycol to improve their...
Everolimus (Eve) is an FDA approved drug that inhibits mammalian target of rapamycin (mTOR). It employed in breast cancer treatment even if its responsiveness controversial. In attempt to increase Eve effectiveness, we have developed a novel nanoformulation exploiting H-ferritin nanocages (HEve) improve subcellular delivery. We took advantage the natural tumor targeting H-Ferritin, which mediated by transferrin receptor-1 (TfR1). Breast cells overexpressing TfR-1 were successfully recognized...
Abstract Background Breast cancer Patient Derived Organoids (PDO) have been demonstrated to be a reliable model study that promised replace and reduce the use of animals in pre-clinical research. They displayed concordance with tissue origin, resuming its heterogenicity representing good platform develop approaches personalized medicines. Although obtain PDOs from mammary tumour, was very challenging process, several ongoing studies evaluated them as efficacy, sensitivity specificity new...
Bisdemethoxycurcumin (BDC) might be an inflammation inhibitor in Alzheimer's Disease (AD). However, BDC is almost insoluble water, poorly absorbed by the organism, and degrades rapidly. We thus developed a new nanoformulation of based on H-Ferritin nanocages (BDC-HFn).We tested BDC-HFn solubility, stability, ability to cross blood-brain barrier (BBB) model. effect AD control (CTR) PBMCs evaluate transcriptomic profile RNA-seq.We with diameter 12 nm improve solubility stability. The...
Brain metastasis (BM) represents a clinical challenge for patients with advanced HER2 + breast cancer (BC). The monoclonal anti-HER2 antibody trastuzumab (TZ) improves survival of BC patients, but it has low central nervous system penetrance, being ineffective in treating BM. Previous studies showed that ferritin nanoparticles (HFn) may cross the blood brain barrier (BBB) through binding to transferrin receptor 1 (TfR1). However, whether this efficacy promoting trans-BBB delivery TZ and...
Protein nanocages represent an emerging candidate among nanoscaled delivery systems. Indeed, they display unique features that proved to be very interesting from the nanotechnological point of view such as uniform structure, stability in biological fluids, suitability for surface modification insert targeting moieties and loading with different drugs dyes. However, one main concerns regards production recombinant proteins E. coli, which leads a product high endotoxin contamination, resulting...
Neoadjuvant chemotherapy has been established as the standard of care for HER2-positive breast cancer since it allows down-staging, up to pathological complete response. The in neoadjuvant setting is a combination highly cytotoxic drugs such anthracyclines and anti-HER2 monoclonal antibody. Despite this cocktail response 50%, their co-administration strongly limited by intrinsic cardiotoxicity. Therefore, only sequential administration treatment allowed. Here, we propose anthracycline...
Assessment of inflammatory bowel disease (IBD) currently relies on aspecific clinical signs inflammation. Specific imaging the diseased regions is still lacking. Here, we investigate mucosal addressin cell adhesion molecule 1 (MAdCAM-1) as a reliable and specific endothelial target for engineered nanoparticles delivering agents to obtain an exact mapping foci.We generated nanodevice composed PLGA-PEG coupled with anti-MAdCAM-1 antibody half-chains loaded quantum dots (P@QD-MdC NPs). Bowel...
Indocyanine green (ICG) is a near infrared fluorescent tracer used in image-guided surgery to assist surgeons during resection. Despite appearing as very promising tool for surgical oncology, its employment this area limited lymph node mapping or laparoscopic surgery, it lacks tumor targeting specificity. Recently, nanoformulation of dye has been proposed with the aim toward specificity order expand oncology. This nanosystem constituted by 24 monomers H-Ferritin (HFn), which self-assemble...
Biological nanoparticles, such as proteins and extracellular vesicles, are rapidly growing nanobased drug-delivery agents due to their biocompatibility, high loading efficiency, bioavailability. However, most of the candidates emerging preclinically hardly confirm potential when entering clinical trials. Among other reasons, this is low control synthesis processes limited characterization immunoreactivity profiles. Here, we propose a combined method that allow us fully characterize...
Abstract Despite the advent of numerous targeted therapies in clinical practice, anthracyclines, including doxorubicin (DOX), continue to play a pivotal role breast cancer (BC) treatment. DOX directly disrupts DNA replication, demonstrating remarkable efficacy against BC cells. However, its non-specificity toward cells leads significant side effects, limiting utility. Interestingly, can also enhance antitumor immune response by promoting immunogenic cell death cells, thereby facilitating...
We investigated the relevance of encapsulation in H-ferritin nanocages (HFn) determining an improved tumor-targeted delivery indocyanine green (ICG). Since from previous experiments, administration HFn loaded with ICG (HFn-ICG) resulted increased fluorescence signal ICG, our aim was to uncover if nanoformulation could have a major role driving specific targeting dye tumor or rather protective action on ICG's fluorescence. Here, we took advantage combined analysis involving ultrahigh...
Extensive intraductal component (EIC) associated to early breast cancer could increase the risk locoregional recurrence, but its impact on distant metastases is still unclear. The aim of present study was assess role EIC 5-year survival outcomes in patients affected by treated with breast-conserving surgery.A total 414 consecutive a minimum follow-up 60 mo were collected from January 2007 December 2015. Disease-free (DFS), metastasis-free (DMFS), and recurrence-free at 5 y assessed...
Indocyanine green (ICG) is one of the most commonly used fluorophores in near-infrared fluorescence-guided techniques. However, molecule prone to form aggregates saline solution with a limited photostability and moderate fluorescence yield. ICG was thus formulated using protein-based nanoparticles H-ferritin (HFn) order generate new nanostructure, HFn-ICG. In this study, an ultrahigh performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) system employed develop validate...