A5058902689- Extracellular vesicles in disease
- Spectroscopy Techniques in Biomedical and Chemical Research
- Advanced biosensing and bioanalysis techniques
- Gold and Silver Nanoparticles Synthesis and Applications
- Advancements in Transdermal Drug Delivery
- Biosensors and Analytical Detection
- RNA Interference and Gene Delivery
- MicroRNA in disease regulation
- Digital Imaging for Blood Diseases
- Lipid Membrane Structure and Behavior
- Neuroinflammation and Neurodegeneration Mechanisms
- Spectroscopy and Chemometric Analyses
- Skin and Cellular Biology Research
- Skin Protection and Aging
- Effects of Radiation Exposure
- Nonmelanoma Skin Cancer Studies
- Bee Products Chemical Analysis
- 3D Printing in Biomedical Research
- Protein Hydrolysis and Bioactive Peptides
- Nanoparticle-Based Drug Delivery
- Phytase and its Applications
- SARS-CoV-2 and COVID-19 Research
- Mycotoxins in Agriculture and Food
- Thermal properties of materials
- Contact Dermatitis and Allergies
Don Carlo Gnocchi Foundation
2016-2025
Mylan (Switzerland)
2014-2015
International Flame Research Foundation
2015
University of Milan
1985-2011
The pandemic of COVID-19 is continuously spreading, becoming a worldwide emergency. Early and fast identification subjects with current or past infection must be achieved to slow down the epidemiological widening. Here we report Raman-based approach for analysis saliva, able significantly discriminate signal patients by from healthy and/or infection. Our results demonstrated differences in saliva biochemical composition three experimental groups, modifications grouped specific attributable...
The use of exosomes for diagnostic and disease monitoring purposes is becoming particularly appealing in biomedical research because the possibility to study directly biological fluids some features related organs from which originate. A paradigmatic example are brain-derived that can be found plasma used as a direct read-out status central nervous system (CNS). Inspired by recent remarkable development plasmonic biosensors, we have designed surface plasmon resonance imaging (SPRi) assay...
Abstract Extracellular vesicles (EVs) from mesenchymal stromal cells (MSC) are emerging as valuable therapeutic agents for tissue regeneration and immunomodulation, but their clinical applications have so far been limited by the technical restraints of current isolation characterisation procedures. This study shows first time successful application Raman spectroscopy label-free, sensitive reproducible means carrying out routine bulk MSC-derived before use in vitro or vivo , thus promoting...
Extracellular vesicles (EVs) from a variety of stem cell sources are believed to harbour regenerative capacity, which may be exploited for therapeutic purposes. Because EV interaction with other soluble secreted factors, activity depend on the employed purification method, limits cross-study comparisons and development. Raman spectroscopy (RS) is quick easy method assess purity composition, giving in-depth biochemical overview preparation. Hereby, we show how this can used characterise EVs...
ABSTRACT Small extracellular vesicles (sEVs) present fairly distinctive lipid membrane features in the environment. These include high curvature, lipid‐packing defects and a relative abundance lipids such as phosphatidylserine ceramide. sEV could be then considered “universal” marker, alternative or complementary to traditional, characteristic, surface‐associated proteins. Here, we introduce use of membrane‐sensing peptides new, highly efficient ligands directly integrate capturing analysis...
Heamatopoietic cancer cells from patients were objectively and accurately recognized by high-resolution Raman imaging their characteristic spectra.
Abstract Amyotrophic Lateral Sclerosis (ALS) is a neurodegenerative disease leading to progressive and irreversible muscle atrophy. The diagnosis of ALS time-consuming complex, with the clinical neurophysiological evaluation accompanied by monitoring progression long procedure for discrimination similar diseases. delayed strongly slows potential development adequate therapies time frame prompt intervention. discovery new biomarkers could improve diagnosis, as well therapeutic rehabilitative...
Abstract Alzheimer disease (AD) is the most common form of dementia in elderly, progressively affecting cognitive functions with a complex diagnostic procedure that limits time for prompt intervention. In this study we optimized reliable protocol analysis AD patients and healthy subjects' serum using Surface Enhanced Raman Spectroscopy (SERS), taking into consideration effect different variables on final spectra, analyzed compared through multivariate correlated hippocampus volume. As...
The characterization of nanoparticle-based drug-delivery systems represents a crucial step in achieving comprehensive overview their physical, chemical, and biological features evaluating efficacy safety systems. We propose Raman Spectroscopy (RS) for the liposomes (LPs) to be tested control neuroinflammation microglial dysfunctions Glioblastoma multiforme Alzheimer's disease. Drug-loaded LPs were functionalized cross blood-brain barrier guarantee localized controlled drug release. spectra...
Casein phosphopeptides (CPPs) form aggregated complexes with calcium phosphate and induce Ca 2+ influx into HT‐29 cells that have been shown to be differentiated in culture. The relationship between the aggregation of CPPs assessed by laser light scattering their biological effect was studied using β‐CN(1–25)4P α s1 ‐CN(59–79)5P, commercial mixture CPP DMV, ‘cluster sequence’ pentapeptide, typical CPPs, dephosphorylated β‐CN(1–25)4P, [β‐CN(1–25)0P]. found be: (a) maximal null sequence’; (b)...
Skeletal muscle regeneration relies on the tightly temporally regulated lineage progression of stem/progenitor cells (MPCs) from activation to proliferation and, finally, differentiation. However, with aging, MPC is disrupted and delayed, ultimately causing impaired regeneration. Extracellular vesicles (EVs) have attracted broad attention as next-generation therapeutics for promoting tissue As a next step toward clinical translation, strategies manipulate EV effects downstream cellular...
We present a new 3D surface-enhanced Raman spectroscopy substrate made of branched gold nanoparticles supported on ZnO tetrapods that was proved to be effective in different biomedical application such as drug detection and cancer cells analysis.
Extracellular Vesicles (EVs) and Conditioned Medium (CM) are promising cell-free approaches to repair damaged diseased tissues for regenerative rehabilitation purposes. They both entail several advantages, mostly in terms of safety handling, compared the cell-based treatment. Despite growing interest EVs CM preparations, light a clinical translation, number aspects still need be addressed mainly because limits reproducibility reliability proposed protocols. Raman spectroscopy (RS) is...