A5067750947- Osteoarthritis Treatment and Mechanisms
- Bone fractures and treatments
- Orthopaedic implants and arthroplasty
- Bone Tissue Engineering Materials
- Knee injuries and reconstruction techniques
- Mesenchymal stem cell research
- Tissue Engineering and Regenerative Medicine
- Total Knee Arthroplasty Outcomes
- Muscle Physiology and Disorders
- Periodontal Regeneration and Treatments
- Orthopedic Infections and Treatments
- Tendon Structure and Treatment
- Lymphatic System and Diseases
- Corneal Surgery and Treatments
- Immune cells in cancer
- Wound Healing and Treatments
- Cell Adhesion Molecules Research
- Heterotopic Ossification and Related Conditions
- TGF-β signaling in diseases
- Exercise and Physiological Responses
- Polymer composites and self-healing
- Bone Metabolism and Diseases
- Cellular Mechanics and Interactions
- Connective Tissue Growth Factor Research
- Proteoglycans and glycosaminoglycans research
University of Oregon
2021-2025
VA Portland Health Care System
2022-2025
Atlanta VA Medical Center
2019-2024
Georgia Institute of Technology
2014-2024
The Wallace H. Coulter Department of Biomedical Engineering
2009-2024
Emory University
2009-2024
Veterans Health Administration
2016-2024
Oregon Health & Science University
2024
Oregon Center For Applied Science (United States)
2024
Impact Technology Development (United States)
2024
We examine the shape memory effect in polymer networks intended for biomedical, and specifically cardiovascular, applications. The polymers were synthesized by photopolymerization from a tert-butyl acrylate monomer with diethyleneglycol diacrylate crosslinker. Three-point flexural tests used to systematically investigate thermomechanics of storage (predeformation) recovery. glass transition temperature, T(g), was determined be approximately 65 degrees C. show 100% strain recovery, at low...
Accurate and tunable: The title compounds can detect reactive oxygen species (ROS) in cell culture, tissue explants, for the first time vivo. hydrocyanines are synthesized by reduction of cyanine dyes with NaBH4. They accurately nanomolar levels ROS, have excellent stability against autoxidation, tunable emission wavelengths range 560–830 nm. Detailed facts importance to specialist readers published as "Supporting Information". Such documents peer-reviewed, but not copy-edited or typeset....
Significance Nanoparticle-mediated delivery of siRNA to hepatocytes has treated disease in humans. However, systemically delivering RNA drugs nonliver tissues remains an important challenge. To increase the number nanoparticles that could be studied vivo, we designed a high-throughput method measure how >100 delivered mRNA was translated into functional protein vivo. We quantified >250 lipid (LNPs) identifying two LNPs deliver endothelial cells. One codelivered Cas9 and single-guide...
New vascular network formation is a critical step in the wound healing process and primary limiting factor functional tissue regeneration. Like many tissues, neovascular networks have been shown vitro to be highly sensitive mechanical conditions; however, effects of matrix deformations on remodeling engineered regeneration vivo not evaluated. We quantified early delayed loading growth rat model large bone defect using compliant fixation plates that were unlocked allow transfer ambulatory...
Abstract Introduction Micronized dehydrated human amnion/chorion membrane (μ-dHACM) is derived from donated placentae and has anti-inflammatory, low immunogenic anti-fibrotic properties. The objective of this study was to quantitatively assess the efficacy μ-dHACM as a disease modifying intervention in rat model osteoarthritis (OA). It hypothesized that intra-articular injection would attenuate OA progression. Methods Lewis rats underwent medial meniscal transection (MMT) surgery induce OA....
Extracellular matrix deformation temporally regulates angiogenesis and upstream angiogenic mechanosensitive pathways.
Volumetric muscle loss (VML) overwhelms the innate regenerative capacity of mammalian skeletal (SkM), leading to numerous disabilities and reduced quality life. Immune cells are critical responders injury guide tissue resident stem cell– progenitor-mediated myogenic repair. However, how immune cell infiltration intercellular communication networks with altered following VML drive pathological outcomes remains underexplored. Herein, we contrast cellular molecular mechanisms injuries that...
Extremity injuries involving large bone defects with concomitant severe muscle damage are a significant clinical challenge often requiring multiple treatment procedures and possible amputation. Even if limb salvage is achieved, patients typically left short- long-term disabilities. Current preclinical animal models do not adequately mimic the severity, complexity, loss of function characteristic these composite injuries. The objectives this study were to establish injury model that combines...
The goal of this study was to determine the threshold for a critically sized, nonhealing muscle defect by characterizing key components in balance between fibrosis and regeneration as function injury size mouse quadriceps. There is currently limited understanding what leads sized which regenerative are functionally impaired. With substantial increase preclinical VML models testbeds tissue engineering therapeutics, defining critical injuries will be instrumental therapeutic efficacy potential...
Localized intra-articular delivery of anti-inflammatory proteins can reduce inflammation in osteoarthritis but poses a challenge because raid clearance within few hours injection. A new class polymer is developed that forms self-assembled nanoparticles ranging from 300 to 900 nm and demonstrates particle size dependent prolonged retention joint spaces compared bolus protein over period 14 d.
Bone marrow derived mesenchymal stem cells (MSCs) are regularly utilized for translational therapeutic strategies including cell therapy, tissue engineering, and regenerative medicine frequently used in preclinical mouse models both mechanistic studies screening of new based therapies. Current methods to culture murine MSCs (mMSCs) select rapidly dividing colonies require long-term expansion. These thus months generate sufficient numbers feasibility a lab setting the populations often have...
Despite progress in bone tissue engineering, the healing of critically sized diaphyseal defects remains a clinical challenge. A stem cell-based approach is an attractive alternative to current treatment techniques. The objective this study was examine ability adult cells enhance formation when co-delivered with osteoinductive factor morphogenetic protein-2 (BMP-2) biologically functionalized hydrogel. First, adipose and marrow-derived mesenchymal (ADSCs BMMSCs) were screened for their...
High velocity impact injuries can often result in loss of large skeletal muscle mass, creating defects devoid matrix, cells, and vasculature. Functional regeneration within these regions volumetric (VML) continues to be a significant clinical challenge. Large cell-seeded, space-filling tissue-engineered constructs that may augment require adequate vascularization maintain cell viability. However, the long-term effect improved addition myoblasts vascularized have not been determined VMLs....
Current strategies for regeneration of large bone fractures yield limited clinical success mainly due to poor integration and healing. Multidisciplinary approaches in design development functional tissue engineered scaffolds are required overcome these translational challenges. Here, a new generation hyperelastic (HB) implants, loaded with superparamagnetic iron oxide nanoparticles (SPIONs), 3D bioprinted their regenerative effect on non-healing is studied. Scaffolds the geometry that...
Monoclonal antibodies (mAb) have had a transformative impact on treating cancers and immune disorders. However, their use is limited by high development time monetary cost, manufacturing complexities, suboptimal pharmacokinetics, availability of disease-specific targets. To address some these challenges, we developed an entirely synthetic, multivalent, Janus nanotherapeutic platform, called Synthetic Nanoparticle Antibodies (SNAbs). SNAbs, with phage-display-identified cell-targeting ligands...