A5080019571- Bone Tissue Engineering Materials
- Bone fractures and treatments
- Orthopaedic implants and arthroplasty
- Osteoarthritis Treatment and Mechanisms
- Mesenchymal stem cell research
- Tissue Engineering and Regenerative Medicine
- Periodontal Regeneration and Treatments
- Bone health and osteoporosis research
- Electrospun Nanofibers in Biomedical Applications
- 3D Printing in Biomedical Research
- Knee injuries and reconstruction techniques
- Bone Metabolism and Diseases
- Bone health and treatments
- Total Knee Arthroplasty Outcomes
- Bone and Dental Protein Studies
- Cellular Mechanics and Interactions
- Bone and Joint Diseases
- Muscle Physiology and Disorders
- Dental Implant Techniques and Outcomes
- Angiogenesis and VEGF in Cancer
- Orthopedic Infections and Treatments
- Shoulder Injury and Treatment
- Tendon Structure and Treatment
- Inflammatory mediators and NSAID effects
- Orthopedic Surgery and Rehabilitation
University of Oregon
2018-2024
Impact Technology Development (United States)
2019-2024
Georgia Institute of Technology
2014-2023
Case Western Reserve University
2021
Cornell University
2021
University of California, Davis
2021
Oregon Center For Applied Science (United States)
2019-2020
Oregon Health & Science University
2020
Synthetic Biologics (United States)
2020
Eugene Research Institute
2020
Skeletal development and turnover occur in close spatial temporal association with angiogenesis. Osteoblasts are ideally situated bone to sense oxygen tension respond hypoxia by activating the hypoxia-inducible factor α (HIFα) pathway. Here we provide evidence that HIFα promotes angiogenesis osteogenesis elevating VEGF levels osteoblasts. Mice overexpressing osteoblasts through selective deletion of von Hippel–Lindau gene (Vhl) expressed high Vegf developed extremely dense, heavily...
Cellular activity at the center of tissue-engineered constructs in static culture is typically decreased relative to construct periphery because transport limitations. We have designed a tissue system that perfuses medium through three-dimensional (3D) porous cellular improve nutrient delivery and waste removal within constructs. This study examined effects perfusion rate on cell viability, proliferation, gene expression cell-seeded 3D bone scaffolds. Human trabecular scaffolds were seeded...
Abstract A murine segmental femoral bone graft model was used to show the essential role of donor periosteal progenitor cells in healing. Transplantation live harvested from Rosa 26A mice showed that ∼70% osteogenesis on attributed expansion and differentiation cells. Furthermore, engraftment BMP-2-producing marrow stromal nonvital allografts marked increases cortical incorporation neovascularization, suggesting gene-enhanced, tissue engineered functional periosteum may improve allograft...
The pathogenesis of osteoarthritis (OA) is poorly understood, and therapeutic approaches are limited to preventing progression the disease. Recent studies have shown that exosomes play a vital role in cell-to-cell communication, many age-related diseases. Molecular profiling synovial fluid derived exosomal miRNAs may increase our understanding OA lead discovery novel biomarkers targets. In this article we report first characterization from human fluid. share similar characteristics (size,...
Significance Orthopedic implant infections require long-term antibiotic therapy and surgical debridement to successfully retain the implant; however, therapeutic failure can lead removal. Here an injectable PEG-based hydrogel that adheres exposed tissue fracture surfaces is engineered deliver antimicrobial enzyme lysostaphin infected, implant-fixed, mouse femoral fractures. Lysostaphin encapsulation within enhances stability while providing enhanced antibiofilm activity serving as a...
Osteopontin (OPN) is abundantly expressed in human calcified arteries. To examine the role of OPN vascular calcification, mutant mice were crossed with matrix Gla protein (MGP) mice. Mice deficient MGP alone (MGP−/− OPN+/+) showed calcification their arteries as early 2 weeks (wk) after birth (0.33 ± 0.01 mmol/g dry weight), and expression was greatly up-regulated compared wild-types. accumulated adjacent to mineral colocalized surrounding cells media. Cells synthesizing lacked smooth muscle...
Small animal models of osteoarthritis are often used for evaluating the efficacy pharmacologic treatments and cartilage repair strategies, but noninvasive techniques capable monitoring matrix-level changes limited by joint size low radiopacity soft tissues. Here we present a technique imaging at micrometer-level resolution based on detecting equilibrium partitioning an ionic contrast agent via microcomputed tomography. The approach exploits electrochemical interactions between molecular...
Objective— The hemodynamic environment is a determinant of susceptibility to atherosclerosis in the vasculature. Although mouse models are commonly used studies, little known about local variations wall shear stress (WSS) and whether levels WSS comparable those humans. objective this study was determine values aorta relate these expression gene products associated with atherosclerosis. Methods Results— Using micro-CT ultrasound methodologies we developed computational fluid dynamics model...
Abstract An 8‐mm rat segmental defect model was used to evaluate quantitatively the ability of longitudinally oriented poly(L‐lactide‐ co ‐D,L‐lactide) scaffolds with or without growth factors promote bone healing. BMP‐2 and TGF‐β3, combined RGD‐alginate hydrogel, were co‐delivered femoral defects within polymer at a dose previously shown synergistically induce ectopic mineralization. A novel modular composite implant design achieve reproducible stable fixation, provide window for...
Transgenic mouse models are increasingly being used to investigate the functions of specific growth factors or matrix proteins design therapeutic strategies for controlling blood vessel growth. However, available methodologies evaluating angiogenesis and arteriogenesis in these limited by animal size, user subjectivity, power visualize three-dimensional networks, capability employ a vigorous quantitative analysis. In this study, we employed contrast-enhanced microcomputed tomography imaging...
New vascular network formation is a critical step in the wound healing process and primary limiting factor functional tissue regeneration. Like many tissues, neovascular networks have been shown vitro to be highly sensitive mechanical conditions; however, effects of matrix deformations on remodeling engineered regeneration vivo not evaluated. We quantified early delayed loading growth rat model large bone defect using compliant fixation plates that were unlocked allow transfer ambulatory...
Abstract Skeletal trauma and impaired skeletal healing is commonly associated with diminished vascularity. Hypoxia inducible factor alpha (HIF‐1) a key transcription responsible for activating angiogenic factors during development tissue repair. Small molecule inhibitors of the prolyl hydroxylase enzyme (PHD), degrading HIF‐1, have been shown to activate are effective in inducing angiogenesis. Here we examined effects several commercially available PHD on bone marrow mesenchymal stromal...
Interfacial zones between tissues provide specialized, transitional junctions central to normal tissue function. Regenerative medicine strategies focused on multiple cell types and/or bi/tri-layered scaffolds do not continuously graded interfaces, severely limiting the integration and biological performance of engineered substitutes. Inspired by bone-soft interface, we describe a biomaterial-mediated gene transfer strategy for spatially regulated genetic modification differentiation primary...