A5054206882- Mesenchymal stem cell research
- Tissue Engineering and Regenerative Medicine
- Osteoarthritis Treatment and Mechanisms
- Extracellular vesicles in disease
- Pain Mechanisms and Treatments
- Periodontal Regeneration and Treatments
- Bone Tissue Engineering Materials
- RNA Interference and Gene Delivery
- Sarcoma Diagnosis and Treatment
- Nerve injury and regeneration
- Electrospun Nanofibers in Biomedical Applications
- Monoclonal and Polyclonal Antibodies Research
- Bone Tumor Diagnosis and Treatments
- Neuropeptides and Animal Physiology
- MicroRNA in disease regulation
- Mast cells and histamine
- Pluripotent Stem Cells Research
- Ocular Oncology and Treatments
- Immune Cell Function and Interaction
- Adipose Tissue and Metabolism
- Oral and Maxillofacial Pathology
- Bone fractures and treatments
- Cancer-related gene regulation
- HIV Research and Treatment
- Cancer Cells and Metastasis
University of Milan
2016-2025
Istituto Ortopedico Galeazzi
2016-2025
Istituti di Ricovero e Cura a Carattere Scientifico
2013-2023
Multidisciplinary Digital Publishing Institute (Switzerland)
2019
Sapienza University of Rome
2019
Orthopedic Institute
2016-2019
Institute for the Chemistry of OrganoMetallic Compounds
2019
Università degli Studi del Piemonte Orientale “Amedeo Avogadro”
2016
Istituto Nazionale di Fisica Nucleare, Sezione di Milano
2013-2015
Beth Israel Deaconess Medical Center
1998
Abstract Painful neuropathy is one of the complications diabetes mellitus that adversely affects patients’quality life. Pharmacological treatments are not fully satisfactory, and novel approaches needed. In a preclinical mouse model effect both human mesenchymal stromal cells from adipose tissue (hASC) their conditioned medium (hASC-CM) was evaluated. Diabetes induced by streptozotocin. After neuropathic hypersensitivity established, mice were intravenously injected with either 1 × 10 6 hASC...
Abstract Extracellular vesicles (EVs) from mesenchymal stromal cells (MSC) are emerging as valuable therapeutic agents for tissue regeneration and immunomodulation, but their clinical applications have so far been limited by the technical restraints of current isolation characterisation procedures. This study shows first time successful application Raman spectroscopy label-free, sensitive reproducible means carrying out routine bulk MSC-derived before use in vitro or vivo , thus promoting...
Extracellular vesicles (EVs) from a variety of stem cell sources are believed to harbour regenerative capacity, which may be exploited for therapeutic purposes. Because EV interaction with other soluble secreted factors, activity depend on the employed purification method, limits cross-study comparisons and development. Raman spectroscopy (RS) is quick easy method assess purity composition, giving in-depth biochemical overview preparation. Hereby, we show how this can used characterise EVs...
Stem cells hold great promise in tissue engineering for repairing tissues damaged by disease or injury. Mesenchymal stem (MSCs) are multipotent able to proliferate and differentiate into multiple mesodermal such as bone, cartilage, muscle, tendon, fat. We have previously reported that the low-affinity nerve growth factor receptor (L-NGFR CD271) defines a subset of with high proliferative, clonogenic, multipotential differentiation ability adult bone marrow (BM). It has been recently shown...
Abstract Background The Ras-assocation family (RASSF) of tumour suppressor genes (TSGs) contains 10 members that encode proteins containing (RA) domains. Several the RASSF are frequently epigenetically inactivated in cancer, however, their role leukaemia has remained largely uninvestigated. Also, RASSF10 is a predicted gene yet to be experimentally verified. Here we cloned, characterised and demonstrated expression normal human bone marrow. We also determined methylation status CpG islands...
We used a chromosome 3 wide NotI microarray for identification of epigenetically inactivated genes in childhood acute lymphoblastic leukemia (ALL). Three novel demonstrated frequent methylation ALL. PPP2R3A (protein phosphatase 2, regulatory subunit B'', alpha) was frequently methylated T (69%) and B (82%)-ALL. Whilst FBLN2 (fibulin 2) THRB (thyroid hormone receptor, beta) showed B-ALL (58%; 56% respectively), but were less T-ALL (17% both genes). Recently it that BNC1 (Basonuclin 1) MXS1...
The spontaneous expression of neural markers, already demonstrated in bone marrow (BM) mesenchymal stem cells (MSCs), has been considered as evidence the MSCs’ predisposition to differentiate toward lineages, supporting their use cell-based therapy for repair. In this study we have evaluated, by immunocytochemistry, immunoblotting, and flow cytometry experiments, markers undifferentiated MSCs from different sources: human adipose (hASCs), skin-derived (hS-MSCs), periodontal ligament...
Abstract Human mesenchymal stromal cells (MSCs) have been widely investigated both for regenerative medicine and their antinflammatory/immunomodulatory capacity. However, ability to home pathological tissues suggested the development of strategies using MSCs as carrier deliver drug into tumor microenvironment. obtained from different can be loaded in vitro with anti-cancer drugs by a simple procedures. In this report, we studied isolated expanded gingival papilla (GinPa-MSCs), testing uptake...
Interdental papilla are an interesting source of mesenchymal stromal cells (GinPaMSCs), which easy to isolate and expand in vitro. In our laboratory, GinPaMSCs were isolated, expanded, characterized by studying their secretome before after priming with paclitaxel (PTX). The did not affect the growth cancer cell lines tested vitro, whereas primed (GinPaMSCs/PTX) exerted a significant anticancer effect. able uptake then release amounts pharmacologically effective against cells, as demonstrated...
The receptor for IgE (Fc epsilon RI) is a multimeric complex containing one alpha chain, beta chain with four transmembrane domains and homodimer of disulfide-linked gamma-chains. Fc RI gamma-chains form additional dimers the homologous zeta- eta-chains, as part TCR complex. low affinity IgG gamma RIII)2 on NK cells also associated zeta-chains. Here we show that gamma-chain expressed in both group heterogenous homodimers heterodimer bound to zeta. RIIIA three types zeta zeta, notably well....
Abstract Mast cells and basophils, which are activated by IgE allergens through the high affinity receptor (Fc epsilon RI), play a prominent role in anaphylaxis mouse. Mice deficient this become resistant to passive anaphylaxis. As first step developing an vivo model that more closely mimics IgE-mediated responses man, we used combination of transgenic embryonic stem cell technology generate mouse line murine Fc RI alpha-chain has been replaced with its human homologue. We demonstrate here...
Abstract The high affinity IgE receptor (Fc epsilon RI) is a tetrameric hetero-oligomer composed of an alpha chain, beta and two disulfide-linked gamma chains. chain contains four transmembrane (TM) segments long cytoplasmic domains that are thought to play important role in intracellular signaling. We now report the structural characterization sequence complete human gene cDNA. spans approximately 10 kilobases seven exons. There single transcription initiation site preceded by TATA box....
Human mesenchymal stem cells (MSCs) have the potential to differentiate into of connective tissue lineages, including bone, cartilage, fat, muscle and also neurons. In our study we examined phenotypic profile human adipose tissue-derived (hASCs) compared different osteogenic-inductive media assess hASC differentiation. Cells were enzymatically isolated from tissues derived by liposuction several adult donors, purified then expanded in culture. We obtained an abundant yield hASCs with a...
Over the last decade, it has been proved that mesenchymal stem cells (MSCs) elicit anti-inflammatory effects. MSCs from adipose tissue (hASCs) differentiate into of mesodermal lineage and transdifferentiate ectodermal-origin cells. Although there are various etiologies to chronic pain, one common feature is painful states associated with increased inflammation. We believe in hASCs as a therapeutic tool also pathologies involving neuroinflammation neuronal damage. have investigated effect...
Today adipose tissue is not just considered as the primary energy storage organ, but it also recognized an important endocrine and abundant source of mesenchymal stem cells (adipose-derived cells, ASCs). During last decade, several studies have provided preclinical data on safety efficacy ASCs, supporting their use in cell-based therapy for regenerative medicine purposes. Little known about effect obesity ASCs properties. Since differentiation proliferation are determined by niche,...
Gingival tissue is composed of cell types that contribute to the body's defense against many agents in oral environment, wound healing and regeneration. Thanks their easy scarcely invasive withdrawal procedure, interdental papilla provide a good source mesenchymal stromal cells (GinPa-MSCs). We isolated GinPa-MSCs verified ability uptake/release anticancer agent Paclitaxel (PTX).In vitro expanded were characterized for CD markers by FACS, tested differentiation analyzed TEM. Their PTX was...
Abstract Background In the last years, several clinical trials have proved safety and efficacy of adipose-derived stem/stromal cells (ASC) in contrasting osteoarthritis (OA). Since ASC act mainly through paracrine mechanisms, their secretome (conditioned medium, CM) represents a promising therapeutic alternative. ASC-CM is complex cocktail proteins, nucleic acids, lipids released as soluble factors and/or conveyed into extracellular vesicles (EV). Here, we investigate its potential an vitro...