A5035424854- Extracellular vesicles in disease
- MicroRNA in disease regulation
- RNA Interference and Gene Delivery
- Reproductive System and Pregnancy
- Endometriosis Research and Treatment
- Advanced biosensing and bioanalysis techniques
- Cell Adhesion Molecules Research
- Characterization and Applications of Magnetic Nanoparticles
- Pregnancy and preeclampsia studies
- Sperm and Testicular Function
- Circular RNAs in diseases
- Lipid Membrane Structure and Behavior
- Inhalation and Respiratory Drug Delivery
- Nanoplatforms for cancer theranostics
- Transgenic Plants and Applications
- Molecular Biology Techniques and Applications
- Virus-based gene therapy research
- Urologic and reproductive health conditions
- Toxin Mechanisms and Immunotoxins
- CRISPR and Genetic Engineering
- Force Microscopy Techniques and Applications
- Bacterial Infections and Vaccines
- Caveolin-1 and cellular processes
- Nanopore and Nanochannel Transport Studies
- Pregnancy and Medication Impact
HansaBioMed (Estonia)
2013-2024
University of Siena
2016-2024
Siena Biotech (Italy)
2017-2021
San Raffaele University of Rome
2009
The San Raffaele Telethon Institute for Gene Therapy
2004-2006
Abstract Current state‐of‐the‐art tools for analysing extracellular vesicles (EVs) offer either highly sensitive but unidimensional bulk measurements of EV components, or high‐resolution multiparametric single‐particle analyses which lack standardization and appropriate reference materials. This limits the accuracy assessment marker abundance overall distribution amongst individual EVs, finally, understanding true heterogeneity. In this study, we aimed to define standardized operating...
Abstract Communication between embryo and maternal endometrium occurs during a specific time frame in which implantation is possible. Here we demonstrate for the first that conditioned media from non-manipulated human embryos cultured vitro 3 days or up to blastocyst stage contain extracellular vesicles (EVs) with diameter of 50 200 nm bearing traditional microvesicle exosome marker proteins CD63, CD9 ALIX. The embryonic origin these EVs has been confirmed by presence stemness gene...
The mechanical properties of extracellular vesicles (EVs) are known to influence their biological function, in terms of, e.g., cellular adhesion, endo/exocytosis, uptake, and mechanosensing. EVs have a characteristic nanomechanical response which can be probed via force spectroscopy (FS) exploited single them out from nonvesicular contaminants or discriminate between subtypes. However, measuring the characteristics individual FS is labor-intensive time-consuming task, usually limiting this...
Detection of BRAFV600E within cell free tumor DNA (ctDNA) is emerging as a promising means to improve patients' stratification or enable BRAF inhibitor (BRAFi) therapeutic monitoring in minimally invasive manner. Here, we investigated whether extracellular vesicle-(EV)-associated-DNA (EV-DNA) has value an alternative source circulating BRAFV600E. To do so, identified clinical practice-compatible protocol for the isolation EV-DNA and assessed gene status on plasma samples from metastatic...
The relevance of extracellular vesicles (EVs) has grown exponentially, together with innovative basic research branches that feed medical and bioengineering applications. Such attraction been fostered by the biological roles EVs, as they carry biomolecules from any cell type to trigger systemic paracrine signaling or dispose metabolism products. To fulfill their roles, EVs are transported through circulating biofluids, which can be exploited for administration therapeutic nanostructures...
Embryo implantation has been defined as the “black box” of human reproduction. Most knowledge on mechanisms underlining this process derives from animal models, but they cannot always be translated to humans. Therefore, development an in vitro/ex vivo model recapitulating closely and precisely possible fundamental functional features endometrial tissue is very much desirable. Here, we have validated organoids a suitable 3D-model studying epithelial interface for embryo implantation....
Are there differences in the proteomic profile of exosomes isolated from seminal plasma normozoospermic (NSP) and severe asthenozoospermic (SA) men, potentially contributing to sperm features?A relevant group proteins known positively regulate functions were over-represented NSP i.e. cysteine-rich secretory protein-1 (CRISP1), while inhibitory protein glycodelin was enriched SA subjects.Exosomes are secreted along male reproductive tract thought be involved spermatozoa maturation function....
We evaluated the advantages and reliability of novel protocols for enrichment tumor extracellular vesicles (EVs), enabling a blood-based test noninvasive parallel profiling multiple androgen receptor (AR) gene alterations. Three clinically relevant AR variants related to response/resistance standard-of-care treatments (AR-V7 transcript, T878A point mutation amplification) were by digital PCR in 15 samples from patients affected Castration-Resistant Prostate Cancer (CRPC). Plasma was...
Extracellular vesicles (EVs) have attracted considerable interest due to their role in cell-cell communication, disease diagnosis, and drug delivery. Despite potential the medical field, there is no consensus on best method for separating micro- nanovesicles from cell culture supernatant complex biological fluids. Obtaining a good recovery yield preserving physical characteristics critical diagnostic therapeutic use of EVs. The separation single class EVs, such as exosomes, because blood...
Intercellular communication is mediated by extracellular vesicles (EVs), as they enclose selectively packaged biomolecules that can be horizontally transferred from donor to recipient cells. Because all cells constantly generate and recycle EVs, provide accurate timed snapshots of individual pathophysiological status. Since blood plasma circulates through the whole body, it often biofluid choice for biomarker detection in EVs. Blood collection easy minimally invasive, yet reproducible...
Despite the significant advances in last decades, low implantation rate per transferred embryo still remains a major concern assisted reproductive techniques, highlighting need to better characterize endometrial receptivity also by mean of specific biomarkers. Based on physiology and intimate contact with endometrium as tissue interest, this study we developed validated an optimized protocol that uses extracellular vesicles (EVs) recovered from uterine flushings cervical brush, latter never...
Abstract Urine features an ideal source of non-invasive diagnostic markers. Some intrinsic and methodological issues still pose barriers to its full potential as liquid biopsy substrate. Unlike blood, urine concentration varies with nutrition, hydration environmental factors. is enriched EVs from urinary-genital tract, while conservation, purification normalization can introduce bias in analysis EV subsets inter-and intra-individual comparisons. The present study evaluated the methods that...
Extracellular vesicles (EVs) have mostly been investigated as carriers of biological therapeutics such proteins and RNA. Nevertheless, small-molecule drugs natural or synthetic origin also loaded into EVs, resulting in an improvement their therapeutic properties. A few methods employed for EV cargo loading, but poor yield drastic modifications remain unsolved challenges. We tested a different strategy based on temporary pH alteration through incubation EVs with alkaline sodium carbonate,...
The antitumor properties of tumor necrosis factor alpha (TNF) and its efficacy in selective destruction tumor-associated vessels are well known. Besides the TNF protein, gene has been used for therapy cancer shown to induce responses both animal models patients. We show here that therapeutic improved by fusing sequence with those peptides able target vessels, such as CNGRCG or ACDCRGDCFCG. Intramuscular administration plasmid DNA encoding CNGRCG-TNF ACDCRGDCFCG-TNF (pNGR-TNF pRGD-TNF,...
Abstract Extracellular vesicles (EVs) derived from different parts of the male reproductive tract can be internalized by human spermatozoa affecting their maturation and regulating functions. Here we demonstrate that EVs female uptaken sperm affect competence. Primary endometrial cells release with a diameter between 50 350 nm bear standard vesicle exosome marker proteins CD63, CD9, TSG101 ALIX. The uptake dye-labelled cell-derived spermatozoa, quantified as fluorescence intensity, was...
Extracellular vesicles (EVs) are lipid membrane-bound particles involved in cell-to-cell communication through a delivery of regulatory molecules essential for physiological processes. Since EVs efficiently vectorize specific cargo molecules, they have been proposed as suitable vehicles therapeutic agents. Drug loading into can be achieved by active, exogenous strategies or genetic modifications vesicle-producing cells. With the aim to produce conveying proteins, we genetically engineered...
Blood plasma is available with minimal invasive sampling, it has significant diagnostic utility, and a valuable source of extracellular vesicles (EVs). Nevertheless, rich protein content, the presence lipoproteins (LPs) that share similar biophysical properties, relatively low abundance EVs, especially those rare subpopulations, make any downstream application very challenging task. The growing evidence intricate surface interactome association EVs LPs, impose further challenges during EV...