A5085744774- Endometriosis Research and Treatment
- Reproductive System and Pregnancy
- Endometrial and Cervical Cancer Treatments
- Uterine Myomas and Treatments
- Gynecological conditions and treatments
- MicroRNA in disease regulation
- Pregnancy and preeclampsia studies
- Reproductive Biology and Fertility
- Extracellular vesicles in disease
- Reproductive Physiology in Livestock
- Reproductive tract infections research
- Sexual Differentiation and Disorders
- Hormonal and reproductive studies
- Ovarian cancer diagnosis and treatment
- Circular RNAs in diseases
- Ovarian function and disorders
- Estrogen and related hormone effects
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Genetic Syndromes and Imprinting
- Pelvic floor disorders treatments
- Cervical Cancer and HPV Research
- Gut microbiota and health
- Galectins and Cancer Biology
- Apelin-related biomedical research
- AI in cancer detection
University of Tartu
2016-2025
Competence Centre on Health Technologies (Estonia)
2016-2025
Tartu University Hospital
2023
Institute of Clinical Research
2017
How can we study the full transcriptome of endometrial stromal and epithelial cells at single-cell level?By compiling developing novel analytical tools for biopsy, tissue cryopreservation disaggregation, sorting, library preparation, RNA sequencing (RNA-seq) statistical data analysis.Although analyses from various biopsied tissues have been published recently, corresponding protocols human endometrium not described.The frozen-thawed biopsies were fluorescence-activated cell sorted (FACS) to...
Health of reproductive tract is tightly associated with balance microbial communities in this area. Bacterial vaginosis (BV) and vulvovaginal candidiasis (VVC) represent common disturbances vaginal communities. Vaginal discharge due to BV or VVC a very frequent reason for visiting gynaecologist. We aimed evaluate the impact novel evidence-based probiotics on patients. The study group included 89 93 patients (aged 18-50 years) who were recruited into randomised double-blind placebo-controlled...
A prerequisite for successful embryo implantation is adequate preparation of receptive endometrium and the establishment maintenance a viable embryo. The success further relies upon two-way dialogue between uterus. However, molecular bases these preimplantation processes in humans are not well known. We performed genome expression analyses human embryos (n = 128) endometria 8). integrated data with protein-protein interactions order to identify networks within embryo, potential...
Alterations in endometrial DNA methylation profile have been proposed as one potential mechanism initiating the development of endometriosis. However, normal methylome is influenced by cyclic hormonal changes, and menstrual cycle phase-dependent epigenetic signature should be considered when studying disorders. So far, no studies performed to evaluate influences endometriosis-specific pattern at same time.Infinium HumanMethylation 450K BeadChip arrays were used explore profiles tissues from...
Background: The human endometrium undergoes a monthly cycle of tissue growth and degeneration. During the mid-secretory phase, establishes an optimal niche for embryo implantation by regulating cellular composition (e.g., epithelial stromal cells) differentiation. Impaired endometrial development observed in conditions such as polycystic ovary syndrome (PCOS) recurrent failure (RIF) contributes to infertility. Surprisingly, despite importance lining properly developing prior pregnancy,...
Abstract STUDY QUESTION What changes occur in the endometrium during aging, and do they impact fertility? SUMMARY ANSWER Both transcriptome cellular composition of endometrial samples from women advanced maternal age (AMA) are significantly different that young women, suggesting specific epithelial cells may affect receptivity. WHAT IS KNOWN ALREADY Aging is associated with accumulation senescent aging tissues. Reproductive mostly attributed to decline ovarian reserve oocyte quality, whereas...
Abstract Expressional profiling of the endometrium enables personalised timing window implantation (WOI). This study presents and evaluates a novel analytical pipeline based on TAC-seq (Targeted Allele Counting by sequencing) method for endometrial dating. The expressional profiles were clustered, differential expression analysis was performed model development group, using 63 biopsies spanning over proliferative (PE, n = 18), early-secretory (ESE, mid-secretory (MSE, 17) late-secretory...
Current therapeutics of endometriosis focus on hormonal disruption endometriotic lesions (ectopic endometrium, EcE). Recent findings show higher glycolysis utilization in EcE, suggesting non-hormonal strategy for disease treatment that addresses cellular metabolism. Identifying metabolically altered cell types EcE is important targeted metabolic drug therapy without affecting eutopic endometrium (EuE). Here, using single-cell RNA-sequencing, we examine twelve pathways paired samples EuE and...
Normal physiological variables, such as age and gender, contribute to alterations in circulating microRNA (miRNA) expression levels. The changes the female body during menstrual cycle can also be reflected plasma miRNA Therefore, this study aimed determine profile of healthy women assess which miRNAs are derived from blood cells. profiles nine were determined by quantitative real time PCR using Exiqon Human Panel I assays four time-points cycle. This platform was used for studying pooled...
Accumulating data have shown the involvement of microRNAs (miRNAs) in endometriosis pathogenesis. In this study, we used a novel approach to determine endometriotic lesion-specific miRNAs by high-throughput small RNA sequencing paired samples peritoneal lesions and matched healthy surrounding tissues together with eutopic endometria same patients. We found five specific epithelial cells – miR-34c, miR-449a, miR-200a, miR-200b miR-141 showing significantly higher expression compared tissues....
microRNA (miRNA) expression level alterations between endometrial tissue and endometriotic lesions indicate their involvement in endometriosis pathogenesis. However, as both endometrium consist of different cell types various proportions, it is not clear which cells contribute to variability miRNA levels the overall knowledge about cell-type specific ectopic scarce. Therefore, we utilized fluorescence-activated sorting isolate stromal from paired endometrioma biopsies combined with...
Genome-wide association studies in the fields of reproductive medicine and endocrinology are yielding robust genetic variants associated with disease. Integrated genomic, transcriptomic, epigenomic molecular profiling common methodologies used to understand biologic pathways perturbed by these variants. However, resources do not include tissue most relevant many female traits, endometrium, while parameters influencing variability results from its unclear. We investigated sources DNA...
Endometrial CD138+ plasma cells serve as a diagnostic biomarker for endometrial inflammation, and their elevated occurrence correlates positively with adverse pregnancy outcomes. Infertility-related conditions like polycystic ovary syndrome (PCOS) recurrent implantation failure (RIF) are closely associated systemic local chronic inflammatory status, wherein cell accumulation could also contribute to pathology. Current methods quantifying typically involve laborious time-consuming microscopic...
The aetiology of endometriosis is still unclear and to find mechanisms behind the disease development, it important study each cell type from endometrium ectopic lesions independently. objective this was uncover complete mRNA profiles in uncultured stromal cells paired samples endometriomas eutopic endometrium. High-throughput sequencing revealed over 1300 dysregulated genes lesions, including several novel context endometriosis. Functional annotation analysis differentially expressed...
The endometrial microbiota has been linked to several gynecological disorders, including infertility. It shown that the microbial profile of endometrium could have a role in fertilization and pregnancy outcomes. In this study we aim assess community tissue (ET) fluid (EF) samples women receiving vitro (IVF) treatment. We also search for possible associations between chronic endometritis (CE) microbiota.This was cohort involving 25 aged 28 42 years with both primary secondary infertility at...
Abstract STUDY QUESTION Does the molecular composition of uterine fluid extracellular vesicles (UF-EVs) reflect endometrial tissue changes across menstrual cycle? SUMMARY ANSWER Concordance between and UF-EVs exists on miRNA mRNA levels along cycle phases UF-EV surface proteomic signatures suggest EVs originate from several major cell populations. WHAT IS KNOWN ALREADY The clinical value receptivity testing is restricted by invasiveness use only one omics level input. There promising...