Background/aims: Lactobacilli are known to play an important role in the maintenance of health by stimulating natural immunity and contributing balance microflora. However, their chronic periodontitis is unclear. We aimed identify oral lactobacilli periodontally healthy subjects, determine antimicrobial activity against putative pathogens. Methods: A total 238 Lactobacillus isolates from saliva subgingival sites 20 15 subjects were collected. In all, 115 strains identified using rapid...

Introduction: Intestinal lactobacilli have been successfully used as probiotics to treat gastrointestinal disorders, but only limited data are available for the probiotic properties of oral combat diseases. We aimed characterize their potential according international guidelines evaluation probiotics, and select strains health. Methods: The study included 67 salivary subgingival 10 species, isolated from healthy humans. All were identified using amplified ribosomal DNA restriction analysis,...

To profile the seminal microbiome applying next generation sequencing.Semen samples of 67 men were involved in study (21 with and 46 without prostatitis). Seminal microbiomes profiled method that uses combinatorial sequence tags attached to polymerase chain reaction primers amplify ribosomal ribonucleic acid V6 region. Amplified products sequenced using an Illumina paired-end protocol on HiSeq2000 platform.The most abundant phylum semen was Firmicutes, comprising nearly half sequences found...

Health of reproductive tract is tightly associated with balance microbial communities in this area. Bacterial vaginosis (BV) and vulvovaginal candidiasis (VVC) represent common disturbances vaginal communities. Vaginal discharge due to BV or VVC a very frequent reason for visiting gynaecologist. We aimed evaluate the impact novel evidence-based probiotics on patients. The study group included 89 93 patients (aged 18-50 years) who were recruited into randomised double-blind placebo-controlled...

Our aim was to study the initial microbial colonization of newborns by comparing it with their mothers’ vaginal microflora. Nineteen mother-newborn pairs were examined at delivery. We found a close association, both qualitative and quantitative, between individually different microflora mother’s vagina that her newborn. The degree contamination newborn significantly correlated counts microorganisms in mothers. In 85% investigated individual we revealed similar predominant microorganisms....

In persons without clinical symptom it is difficult to assess an impact of probiotics regarding its effect on health. We evaluated the functional efficacy probiotic Lactobacillus fermentum ME-3 in healthy volunteers by measuring influence two different formulations intestinal lactoflora, fecal recovery strain and oxidative stress markers blood urine after 3 weeks consumption.

Background: Vaginal lactobacilli offer protection against recurrent urinary infections, bacterial vaginosis, and vaginal candidiasis.Objective: To characterise the isolated strains for their probiotic properties to compare potential.Methods: The Lactobacillus were from samples by conventional culturing identified sequencing of 16S rDNA fragment.Several functional detected (production hydrogen peroxide lactic acid; antagonistic activity Escherichia coli, Candida albicans, glabrata,...

Very few studies have analyzed how the composition of mother's microbiota affects development infant's gut and oral during first months life. Here, present in mothers' gut, vagina, breast milk, cavity, mammary areola were compared with their infants over six following birth. Samples collected from aforementioned body sites seven mothers nine at three different time points a 6-month period. Each sample was 16S rRNA gene sequencing. The harbored distinct microbial communities that had low...

Only a few microbial studies have conducted in IVF (in vitro fertilization), showing the high-variety bacterial contamination of culture media to cause damage or even loss cultured oocytes and embryos. We aimed determine prevalence counts bacteria samples, associate them with clinical outcome.The studied samples from 50 infertile couples included: raw (n = 48), processed 49) incubated 50) sperm 50). The full microbiome was analyzed by 454 pyrosequencing quantitative analysis real-time PCR....

The composition of centenarians’ gut microbiota has consistently been used as a model for healthy aging studies. However, there is an incomplete understanding how childhood living conditions and eating habits affect the development gastrointestinal in centenarians with good cognitive functions. We compared well oldest-old group young people group. richness diversity abundance hereditary environmental microbes were higher longevity than people. People ate more potatoes cereal products. In...

Despite the considerable progress made in assisted reproductive technologies (ART), implantation rate of transferred embryos remains low and many cases, reasons for failure remain unclear. We aimed to determine potential impact female male partners' tract microbiome composition on ART outcome.The couples (n = 97) healthy 12) were recruited into study. The smaller group underwent a careful selection according their general health criteria. Both vaginal semen samples subjected 16S rDNA...

Abstract BACKGROUND Inflammatory prostatitis patients are characterized by oxidative stress (OxS) at local and systemic levels. Less is known about the occurrence of OxS in case other frequent male genital tract disorders. METHODS The study included 196 men: controls (n = 28), asymptomatic inflammatory (NIH category IV) 21), non‐inflammatory IIIb) chronic prostatitis/chronic pelvic pain syndrome (CP/CPPS) 48), IIIa) CP/CPPS 44), benign prostate hyperplasia (BPH) 33), with BPH NIH IV 22). In...

Bariatric surgery is considered to be the most effective treatment option for weight reduction in obese patients. Abdominal obesity frequently accompanied by metabolic syndrome (MS). Adipokines are cell signaling proteins that have direct impact upon homeostasis. The purpose of this analysis was evaluate effect bariatric surgery, including laparoscopic sleeve gastrectomy (LSG) and gastric bypass (LRYGB) on adipokine levels profile as well MS status type 2 diabetes (T2D).We analyzed...

The aim of the study was to find out correlation between white blood cell (WBC) counts in semen and quantitative composition seminal microflora, establish minimum WBC count associated with significant bacteriospermia. research included 159 men different their semen, 84 them chronic prostatitis/chronic pelvic pain syndrome. Semen samples were cultivated quantitatively for detecting anaerobic, microaerophilic aerobic bacteria. Bryan-Leishman stained slides used semen. Seminal fluid colonized...

Background: Apical periodontitis (AP) is an inflammatory disease affecting periradicular tissues. It a widespread condition but its etiopathogenetic mechanisms have not been completely elucidated and the current treatment options are always successful. Purpose: To compare oxidative stress (OxS) levels in saliva endodontium (root canal [RC] contents) patients with different endodontic pathologies endodontically healthy subjects. Patients methods: The study group of this comparison included 22...

To evaluate the occurrence of mycoplasmas in semen chronic prostatitis patients.Genital (Mycoplasma hominis, Mycoplasma genitalium, Ureaplasma urealyticum, parvum) were sought 121 patients [38 National Institutes Health (NIH) category IIIa, 59 NIH IIIb and 24 IV] 40 controls. The commercially available kit IST was applied to samples all 161 men, polymerase chain reaction (PCR) those 60 randomly selected men.Ureaplasmas found study groups (at frequencies ranging from 12% 25%) using test, but...

We investigated the prevalence of oxidative stress in male partners infertile couples displaying different inflammation patterns their genital tract and/or oligospermia. The levels acknowledged markers (8-isoprostanes [8-EPI], diene conjugates, reactive oxygen species-total antioxidant capacity [ROS-TAC] score) were elevated both leukocytospermic men and subjects whose was limited only to expressed prostatic secretion post-massage urine. Oligospermic with severe from oligospermic who had no...

Micro-organisms are tightly integrated into host-microbiota ecosystem. Microbiota of human semen has been studied so far mostly in case infertility or prostatitis. We aimed to reveal possible impact sexual debut on seminal microbiota healthy young men. The study group included 68 men, them 12 men without experience, 11 with single lifetime partner and 45 multiple partners. Basic parameters were similar for all subgroups, no correlation between experience WBC counts was found. A man could...