A5034776080- Proteoglycans and glycosaminoglycans research
- Endometriosis Research and Treatment
- Glycosylation and Glycoproteins Research
- Cell Adhesion Molecules Research
- Estrogen and related hormone effects
- Reproductive System and Pregnancy
- Fibroblast Growth Factor Research
- Cancer Cells and Metastasis
- Uterine Myomas and Treatments
- Protease and Inhibitor Mechanisms
- MicroRNA in disease regulation
- Nitric Oxide and Endothelin Effects
- Cancer-related molecular mechanisms research
- Endometrial and Cervical Cancer Treatments
- Angiogenesis and VEGF in Cancer
- Circular RNAs in diseases
- Cancer, Hypoxia, and Metabolism
- HER2/EGFR in Cancer Research
- Receptor Mechanisms and Signaling
- Connective tissue disorders research
- RNA Research and Splicing
- Cellular transport and secretion
- Congenital Heart Disease Studies
- Immune cells in cancer
- Blood Coagulation and Thrombosis Mechanisms
University Hospital Münster
2016-2025
University of Münster
2013-2025
Klinik für Frauenheilkunde
2013-2023
Transylvania University of Brașov
2022
University of Cagliari
2022
KK Women's and Children's Hospital
2022
Klinik für Schlafmedizin
2009-2021
Zentrum für Kinderheilkunde
2020
Universitätsklinikum Gießen und Marburg
2018-2019
Hudson Institute
2019
Biglycan, a small leucine-rich proteoglycan, is ubiquitous ECM component; however, its biological role has not been elucidated in detail. Here we show that biglycan acts macrophages as an endogenous ligand of TLR4 and TLR2, which mediate innate immunity, leading to rapid activation p38, ERK, NF-κB thereby stimulating the expression TNF-α macrophage inflammatory protein–2 (MIP-2). In agreement, stimulatory effects are significantly reduced TLR4-mutant (TLR4-M), TLR2–/–, myeloid...
Inflammatory breast cancer (IBC), a particularly aggressive form of cancer, is characterized by stem cell (CSC) phenotype. Due to lack targeted therapies, the identification molecular markers IBC major importance. The heparan sulfate proteoglycan Syndecan-1 acts as coreceptor for growth factors and chemokines, modulating inflammation, tumor progression, stemness, thus it may emerge marker IBC.We expression CSC CD44, Notch-1 & -3 EGFR in carcinoma tissues triple negative (n = 13) non-IBC 17)...
Using a high throughput heparan sulfate (HS) isolation and characterization protocol, we have analyzed HS structure in several tissues from mice/mouse embryos deficient biosynthesis enzymes (N-deacetylase/N-sulfotransferase (NDST)-1, NDST-2, C5-epimerase, respectively) mice lacking syndecan-1. The results given us new information regarding with implications on the role of embryonic development. Our main conclusions are as follows. 1) content, disaccharide composition, overall degree N-...
Adult stem cells are thought to be responsible for the high regenerative capacity of human endometrium, and have been implicated in pathology endometriosis endometrial carcinoma. The RNA-binding protein Musashi-1 is associated with maintenance asymmetric cell division neural epithelial progenitor cells. We investigated expression localization endometrial, endometriotic carcinoma tissue specimens 46 patients. qPCR revealed significantly increased mRNA endometrium compared myometrium....
The heparan sulfate proteoglycan syndecan-1 (Sdc1) modulates cell proliferation, adhesion, migration and angiogenesis. Proteinase-mediated shedding converts Sdc1 from a membrane-bound coreceptor into soluble effector capable of binding the same ligands. In breast carcinomas, overexpression correlates with poor prognosis an aggressive phenotype. To distinguish between roles shed forms in cancer progression, human MCF-7 cells were stably transfected plasmids overexpressing wild-type (WT),...
Abstract microRNAs are small endogenous noncoding RNAs, which post‐transcriptionally regulate gene expression. In breast cancer, overexpression of the transmembrane heparan sulfate proteoglycan syndecan‐1, a predicted target oncomiR miR‐10b, correlates with poor clinical outcome. To investigate potential functional relationship miR‐10b and MDA‐MB‐231 MCF‐7 cancer cells were transiently transfected pre‐miR‐10b, syndecan‐1 siRNA or control reagents, respectively. Altered cell behavior was...
ObjectiveTo harmonize standard operating procedures (SOPs) and standardize the recording of associated data for collection, processing, storage human tissues relevant to endometriosis.DesignAn international collaboration involving 34 clinical/academic centers three industry collaborators from 16 countries on five continents.SettingIn 2013, two workshops were conducted followed by global consultation, bringing together 54 leaders in endometriosis research sample processing around...
Abstract Cancer gene therapy requires the design of non-viral vectors that carry genetic material and selectively deliver it with minimal toxicity. Non-viral based on cationic natural polymers can form electrostatic complexes negatively-charged polynucleotides such as microRNAs (miRNAs). Here we investigated physicochemical/biophysical properties chitosan–hsa-miRNA-145 (CS–miRNA) nanocomplexes biological responses MCF-7 breast cancer cells cultured in vitro . Self-assembled CS–miRNA were...
Syndecan-1 (CD138), a heparan sulfate proteoglycan, acts as coreceptor for growth factors and chemokines is molecular marker associated with epithelial-mesenchymal transition during development carcinogenesis. Resistance of Syndecan-1-deficient mice to experimentally-induced tumorigenesis has been linked altered Wnt-responsive precursor cell pools, suggesting potential role in breast cancer stem function. However, the precise mechanism still elusive. Here, we decipher functional impact...