A5076179615- Reproductive System and Pregnancy
- Endometriosis Research and Treatment
- Immune Cell Function and Interaction
- Cytomegalovirus and herpesvirus research
- Reproductive Physiology in Livestock
- COVID-19 Clinical Research Studies
- Ectopic Pregnancy Diagnosis and Management
- Multiple Sclerosis Research Studies
- Urticaria and Related Conditions
- Viral-associated cancers and disorders
- Reproductive Biology and Fertility
- Pregnancy and Medication Impact
- SARS-CoV-2 and COVID-19 Research
- Parvovirus B19 Infection Studies
- COVID-19 Impact on Reproduction
- Mesenchymal stem cell research
- Immunotherapy and Immune Responses
- Cytokine Signaling Pathways and Interactions
- Cell Adhesion Molecules Research
- T-cell and B-cell Immunology
- Herpesvirus Infections and Treatments
- Pregnancy and preeclampsia studies
- Polyomavirus and related diseases
- Dental Research and COVID-19
- Gynecological conditions and treatments
University of Ferrara
2016-2025
University of Catania
2024-2025
Ospedale Garibaldi
2025
Ospedale Sant Antonio
2011-2024
Azienda USL di Bologna
2022
University Hospital Southampton NHS Foundation Trust
2022
Arcispedale Sant'Anna
2015-2021
Policlinico S.Orsola-Malpighi
2017-2020
University of Bologna
2020
Centre of Biotechnology of Sfax
2020
(1) Background: Acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the etiological agent for disease (COVID-19) that has led to a pandemic began in March 2020. The role of SARS-CoV-2 components on innate and adaptive immunity still unknown. We investigated possible implication pathogen-associated molecular patterns (PAMPs)-pattern recognition receptors (PRRs) interaction. (2) Methods: infected Calu-3/MRC-5 multicellular spheroids (MTCSs) with clinical strain evaluated activation RNA...
Different mechanisms mediated by the expression of HLA-class Ib HLA-G products are suggested to account for induction immune tolerance against paternal antigens fetus during pregnancy. Soluble antigens, mainly produced cytotrophoblast cells at materno-fetal interface and circulating in body fluids, show a capacity analogous that membrane-boundstructures inhibit NK cells. In present report we have investigated, using specific ELISA, presence sHLA-G molecules culture supernatants early embryos...
Abstract Communication between embryo and maternal endometrium occurs during a specific time frame in which implantation is possible. Here we demonstrate for the first that conditioned media from non-manipulated human embryos cultured vitro 3 days or up to blastocyst stage contain extracellular vesicles (EVs) with diameter of 50 200 nm bearing traditional microvesicle exosome marker proteins CD63, CD9 ALIX. The embryonic origin these EVs has been confirmed by presence stemness gene...
Hashimoto's thyroiditis (HT) is the most common of all thyroid diseases and characterized by abundant lymphocyte infiltrate impairment, caused various cell- antibody-mediated immune processes. Viral infections have been suggested as possible environmental triggers, but conclusive data are not available. We analyzed presence transcriptional state human herpesvirus 6 (HHV-6) in fine needle aspirates (FNA) peripheral blood mononuclear cells (PBMCs) from 34 HT patients 28 controls, showing that...
Natural killer cells are important in the control of viral infections. However, role NK during severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection has previously not been identified. Peripheral blood from SARS-CoV and SARS-CoV-2 naïve subjects were evaluated for their activation, degranulation, interferon-gamma expression presence spike proteins. K562 lung epithelial transfected with proteins co-cultured cells. The analysis was performed by flow cytometry immune...
Abstract As an inherited disorder characterized by severe pulmonary disease, cystic fibrosis could be considered a comorbidity for coronavirus disease 2019. Instead, current clinical evidence seems to heading in the opposite direction. To clarify whether host factors expressed Cystic Fibrosis epithelia may influence 2019 progression, here we describe expression of SARS-CoV-2 receptors primary airway epithelial cells. We show that angiotensin converting enzyme 2 (ACE2) and localization are...
In human reproduction, embryo implantation is complex and poorly understood. At present, no single markers are used in routine treatment to assay biochemical functions of the embryo. Soluble leukocyte antigen-G (sHLA-G) could be considered a possible marker developmental potential. It localized primarily on extravillous trophoblast, making this antigen potential mediator immune interaction at maternal-fetal interface during gestation.Soluble-HLA-G levels were evaluated by an enzyme-linked...
Abstract Systemic lupus erythematosus (SLE) is an autoimmune disease mainly mediated by the deposit of immune complexes and defects in T lymphocytes antigen‐presenting cells along with a high production T‐helper 2 cytokines. A tolerance‐inducible function nonclassical class Ib human leukocyte antigen (HLA)‐G molecule innate adaptive cellular responses has been reported, suggesting role inflammatory diseases. 14 bp sequence insertion/deletion polymorphism (rs16375) 3′‐untranslated region...
Problem Human Leukocyte Antigen (HLA)‐G is a class Ib gene located in the human major histocompatibility complex (MHC). Several lines of investigation indicate that HLA‐G molecule involved maternal acceptance semi‐allogenic fetus during pregnancy and development tolerance. Expression soluble (sHLA‐G) positively correlated with successful vitro fertilization (IVF) treatments, aberrant expression certain complications pregnancy, such as pre‐eclampsia spontaneous abortion, has been reported....
Abstract Snail mucus is a mixture of active substances commonly thought to have healthy properties for the treatment skin disorders. Although snail an ingredient several cosmetic and para-pharmaceutic products, comprehensive characterization chemical composition biological effects still missing. Crude purified extracts from Helix aspersa muller (HelixComplex) were prepared and, after characterization, tested on in vitro experimental models. Differently what expected, HelixComplex was...
To elucidate the roles of human herpesvirus (HHV)-6 primary unexplained infertile women, a prospective randomized study was conducted on cohort women and control with at least one successful pregnancy. HHV-6 DNA analyzed percentage immune-phenotype resident endometrial Natural Killer (NK) cells, as first line defense towards viral infections, evaluated in biopsies. Cytokine levels uterine flushing samples were analyzed. HHV-6A found 43% biopsies from but not women. On contrary, HHV-6B absent...
Human leukocyte antigen (HLA)-G is a non-classical HLA class I molecule with known immune-modulatory functions. Our group identified subset of human dendritic cells, named DC-10, that induce adaptive interleukin-10-producing T regulatory type 1 (Tr1) cells via the interleukin-10-dependent HLA-G/ILT4 pathway. In this study we aimed at defining role HLA-G in DC-10-mediated Tr1 cell differentiation. We analyzed phenotype, functions, and genetic variations 3' untranslated region locus...
Can the priorities for future research in infertility be identified?The top 10 four areas of male infertility, female and unexplained medically assisted reproduction ethics, access organization care people with fertility problems were identified.Many fundamental questions regarding prevention, management consequences remain unanswered. This is a barrier to improving received by those problems.Potential collated from an initial international survey, systematic review clinical practice...
The control of viral infections in the brain involves activation microglial cells, macrophages that are constantly surveying central nervous system, and production amyloid-beta (Aβ) as an anti-microbial molecule. Recent findings suggest a possible implication HHV-6A AD. We evaluated effect infection on cell expression Aβ status, determined by TREM2, ApoE, cytokines, tau expression.We have infected cells (HMC3, ATCC®CRL-3304), monolayer human peripheral blood monocyte-derived microglia...