A5004918425- Cystic Fibrosis Research Advances
- Neonatal Respiratory Health Research
- MicroRNA in disease regulation
- Advanced biosensing and bioanalysis techniques
- RNA Interference and Gene Delivery
- Tracheal and airway disorders
- Circular RNAs in diseases
- Antimicrobial Peptides and Activities
- Immune Response and Inflammation
- Asthma and respiratory diseases
- Respiratory viral infections research
- Pediatric health and respiratory diseases
- Lipid Membrane Structure and Behavior
- Virus-based gene therapy research
- NF-κB Signaling Pathways
- Sphingolipid Metabolism and Signaling
- Pneumonia and Respiratory Infections
- DNA and Nucleic Acid Chemistry
- Extracellular vesicles in disease
- Congenital Ear and Nasal Anomalies
- Glioma Diagnosis and Treatment
- Carbohydrate Chemistry and Synthesis
- Bacterial biofilms and quorum sensing
- Molecular Sensors and Ion Detection
- Inhalation and Respiratory Drug Delivery
University of Ferrara
2006-2025
University of Verona
2013-2022
Istituto Oncologico Veneto
2017
Azienda Ospedaliera Universitaria Integrata Verona
2017
University of Macerata
2013
Laboratory of Molecular Genetics
2007-2012
Center for Translational Neurophysiology of Speech and Communication
2010
Université de Poitiers
2006
Centre National de la Recherche Scientifique
2006
Cystic Fibrosis Research Foundation
1993-2003
Cell infection by adenovirus serotypes 2 and 5 (Ad2/5) initiates with the attachment of Ad fiber to coxsackievirus receptor (CAR) followed alpha(v) integrin-mediated entry. We recently demonstrated that heparan sulfate glycosaminoglycans (HS GAGs) expressed on cell surfaces are involved in binding Ad2/5 (M. C. Dechecchi, A. Tamanini, Bonizzato, G. Cabrini, Virology 268:382-390, 2000). The role HS GAGs was investigated using extracellular soluble domain 1 CAR (sCAR-D1) heparin as analogues...
Sulfatides have been established recently as ligands for L-selectin, and we investigated whether they trigger transmembrane signals through ligation of L-selectin.We found that sulfatides the increase cytosolic free calcium in neutrophils this effect was strictly dependent on sulfation galactose ring, non-sulfated galactocerebrosides were not stimulatory.Chymotrypsin phorbol12-myristate 13-acetate treatment caused shedding but class I major histocompatibility complex antigens or /32...
The common pathological manifestation of cystic fibrosis (CF) is associated with an excessive lung inflammatory response characterized by interleukin-1β accumulation. CF airway epithelial cells show exacerbated pro-inflammatory to Pseudomonas aeruginosa; however, it unclear whether this heightened intrinsic lacking transmembrane conductance regulator (CFTR). Here we demonstrate that the degree and quality in are supported P. aeruginosa-dependent mitochondrial perturbation, which flagellin...
Abstract As an inherited disorder characterized by severe pulmonary disease, cystic fibrosis could be considered a comorbidity for coronavirus disease 2019. Instead, current clinical evidence seems to heading in the opposite direction. To clarify whether host factors expressed Cystic Fibrosis epithelia may influence 2019 progression, here we describe expression of SARS-CoV-2 receptors primary airway epithelial cells. We show that angiotensin converting enzyme 2 (ACE2) and localization are...
Abstract The P2X7R is an ATP-gated cation channel expressed in hemopoietic cells that participates both cell proliferation and apoptosis. Expression function of the have been associated with clinical course patients affected by chronic lymphocytic leukemia (CLL). Functional variants causing loss-of-function identified, namely, polymorphisms 1513A>C (E496A), 1729T>A (I568N), 946G>A (R307Q). Here we investigated other nonsynonymous located either extracellular portion...
Abstract Recent data show that proinflammatory stimuli may modify significantly ion transport in the airway epithelium and therefore properties of surface fluid. We have studied effect IL-4, a cytokine involved pathogenesis asthma, on transepithelial human bronchial vitro. Incubation polarized epithelial cells with IL-4 for 6–48 h causes marked inhibition amiloride-sensitive Na+ channel as measured short circuit current experiments. On other hand, evokes 2-fold increase activated by cAMP...
IL-8 released from bronchial epithelial cells infected with Pseudomonas aeruginosa plays a crucial role in the chronic lung pathology of patients affected by cystic fibrosis. Novel anti-inflammatory approaches will benefit thorough understanding regulatory mechanisms involved transcription this chemokine to identify potential pharmacological targets. We addressed issue investigating phosphoproteins and factors (TFs) on gene human IB3-1, CuFi-1, Calu-3 cells. P. increased basal...
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Abstract Repeated cycles of infections, caused mainly by Pseudomonas aeruginosa, combined with a robust host immune response and tissue injury, determine the course outcome cystic fibrosis (CF) lung disease. As disease progresses, P. aeruginosa adapts to modifying dramatically its phenotype; however, it remains unclear whether how bacterial adaptive variants their persistence influence pathogenesis development. Using in vitro murine models infection, we showed that CF-adaptive shaped innate...
Patients with glioblastoma (GBM) have a dramatically poor prognosis. The recent REGOMA trial suggested an overall survival (OS) benefit of regorafenib in recurrent GBM patients. Considering the extreme genetic heterogeneity GBMs, we aimed to identify molecular biomarkers predictive differential response drug.Total RNA was extracted from tumor samples patients enrolled trial. Genome-wide transcriptome and micro (mi)RNA profiles were associated patients' OS progression-free survival.In first...
Mitochondrial therapy offers an alternative strategy for attenuation of hyperinflammation in cystic fibrosis lung disease.
ABSTRACT The innate immune response to adenovirus (Ad)-derived gene transfer vectors has been shown initiate immediately after interaction of Ad with respiratory epithelial cells, through the induction extracellular signal-regulated kinase 1 and 2 (ERK1/2) JNK mitogen-activated protein (MAPK), nuclear factor κB (NF-κB), different proinflammatory genes. serotypes or 5 (Ad2/5) enter epithelia initial binding fiber coxsackievirus-adenovirus receptor (CAR) or, alternatively, cell surface heparan...
The biological activity of a combined treatment U251, U373 and T98G glioma cell lines with two anti-miR PNAs, directed against miR‑221 miR‑222 conjugated an ocataarginine tail (R8-PNA-a221 R8-PNA-a222) for efficient cellular delivery, was determined. Apoptosis analyzed, the effect cells either or both PNAs on reversion drug-resistance phenotype assessed in temozolomide-resistant line. Selectivity PNA/miRNA interactions studied by surface plasmon resonance (SPR)-based Biacore analysis....
Peptide nucleic acids (PNAs) are very useful tools for gene regulation at different levels, but in particular the last years their use targeting microRNA (anti-miR PNAs) has provided impressive advancements. In this respect, microRNAs related to repression of cystic fibrosis transmembrane conductance regulator (CFTR) gene, which is defective fibrosis, great importance development new type treatments. paper we propose an anti-miR PNA miR-145, a reported suppress CFTR expression....
Background: High-grade gliomas (HGG) are malignant brain tumors associated with frequent recurrent disease. Clinical management of HGG patients is currently devoid blood biomarkers for early diagnosis, monitoring therapeutic effects and predicting recurrence. Different circulating miRNAs, both free exosomes, described in HGG. We previously identified miR-21, miR-222 miR-124-3p purified from serum exosomes as molecular signature to help pre-operative clinical diagnosis grading gliomas. The...